The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Head-to-tail polymerization of microtubules in vitro. Electron microscope analysis of seeded assembly.
Open accessAbstract
Microtubules are polar structures, and this polarity is reflected in their biased directional growth. Following a convention established previously (G. G. Borisy, 1978, J. Mol. Biol. 124:565--570), we define the plus (+) and minus (-) ends of a microtubule as those equivalent in structural orientation to the distal and proximal ends, respectively, of the A subfiber of flagellar outer doublets. Rates of elongation were obtained for both ends using flagellar axonemes as seeds and porcine brain microtubule protein as subunits. Since the two ends of a flagellar seed are distinguishable morphologically, elongation of each end may be analyzed separately. By plotting rates of elongation at various concentrations of subunit protein, we have determined the association and dissociation rate constants for the plus and minus ends. Under our conditions at 30 degrees C, the association constants were 7.2 X 10(6) M-1 s-1 and 2.25 X 10(6) M-1 s-1 for the plus and minus ends, respectively, and the dissociation constants were 17 s-1 and 7 s-1. From these values and Wegner's equations (1976, J. Mol. Biol. 108:139--150), we identified the plus end of the microtubule as its head and calculated "s," the head-to-tail polymerization parameter. Surprisingly small values (s = 0.07 +/- 0.02) were found. The validity of models of mitosis based upon head-to-tail polymerization (Margolis et al., 1978, Nature (Lond.) 272:450--452) are discussed in light of a small value for s.
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Citations
Cites
Head to tail polymerization of actin
Wegner, A. (1976) · Journal of Molecular Biology · doi:10.1016/s0022-2836(76)80100-3 · Cited by 707
Opposite end assembly and disassembly of microtubules at steady state in vitro
Margolis, R. L., & Wilson, L. (1978) · Cell · doi:10.1016/0092-8674(78)90132-0 · Cited by 570
Arrangement of subunits in flagellar microtubules
Amos, L. A., & Klug, A. (1974) · Journal of Cell Science · doi:10.1242/jcs.14.3.523 · Cited by 514
Borisy, G. G., Marcum, J. M., Olmsted, J. B., Murphy, D. B., & Johnson, K. A. (1975) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1975.tb19196.x · Cited by 423
Self-citationKinetic analysis of microtubule self-assembly in vitro
Johnson, K. A., & Borisy, G. G. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90020-1 · Cited by 265
Self-citationStructural polarity and directional growth of microtubules of Chlamydomonas flagella
Allen, C., & Borisy, G. G. (1974) · Journal of Molecular Biology · doi:10.1016/0022-2836(74)90381-7 · Cited by 259
Self-citationMitotic mechanism based on intrinsic microtubule behaviour
MARGOLIS, R. L., WILSON, L., & KIEFER, B. I. (1978) · Nature · doi:10.1038/272450a0 · Cited by 231
Microtubule assembly: Some possible regulatory mechanisms
Olmsted, J. B., Marcum, J. M., Johnson, K. A., Allen, C. A., & Borisy, G. G. (1974) · Journal of Supramolecular Structure · doi:10.1002/jss.400020230 · Cited by 147
Self-citationAssembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
Binder, L. I., Dentler, W. L., & Rosenbaum, J. L. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.3.1122 · Cited by 114
Directionality of Brain Microtubule Assembly In Vitro
Dentler, W. L., Granett, S. E., Witman, G. B., & Rosenbaum, J. L. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.5.1710 · Cited by 97
Cited by
Dynamic instability of microtubule growth
Mitchison, T., & Kirschner, M. (1984) · Nature · doi:10.1038/312237a0 · Cited by 3,337
Beyond self-assembly: From microtubules to morphogenesis
KIRSCHNER, M. (1986) · Cell · doi:10.1016/0092-8674(86)90318-1 · Cited by 1,362
Walker, R. A., O'Brien, E. T., Pryer, N. K., Soboeiro, M. F., Voter, W. A., Erickson, H. P., & Salmon, E. D. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.4.1437 · Cited by 980
Baas, P. W., Deitch, J. S., Black, M. M., & Banker, G. A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.21.8335 · Cited by 742
Force Generation by Microtubule Assembly/Disassembly in Mitosis and Related Movements
Inoué, S., & Salmon, E. D. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.12.1619 · Cited by 532
Metadata
- Authors
- Bergen, L. G., & Borisy, G. G.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1980-01-01
- Volume
- 84
- Issue
- 1
- Pages
- 141-150
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2034677151
- Cited by
- 278
- References
- 12
- FWCI
- 17.63
- Type
- journal article
- Language
- en
- Open access
- green
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