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Cited by 742Microtubule and mitosis dynamicsAxon Guidance and Neuronal SignalingNeurogenesis and neuroplasticity mechanisms

Polarity orientation of microtubules in hippocampal neurons: uniformity in the axon and nonuniformity in the dendrite

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Baas, P W, Deitch, J S, Black, M M, Banker, G A·1988·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.85.21.8335PMC PMC282424
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Abstract

We have analyzed the polarity orientation of microtubules in the axons and dendrites of cultured rat hippocampal neurons. As previously reported of axons from other neurons, microtubules in these axons are uniform with respect to polarity; (+)-ends are directed away from the cell body toward the growth cone. In sharp contrast, microtubules in the mid-region of the dendrite, approximately 75 microns from the cell body, are not of uniform polarity orientation. Roughly equal proportions of these microtubules are oriented with (+)-ends directed toward the growth cone and (+)-ends directed toward the cell body. At distances within 15 micron of the growth cone, however, microtubule polarity orientation in dendrites is similar to that in axons; (+)-ends are uniformly directed toward the growth cone. These findings indicate a clear difference between axons and dendrites with respect to microtubule organization, a difference that may underlie the differential distribution of organelles within the neuron.

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  • MTs in dendrites have mixed orientations.

    “Unlike in the axon, MTs in dendrites have been shown to be mixed in their orientations (14).”

    From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [14]

    The matching passage is labeled Abstract, which cannot serve as evidence under the stated rule. Our copy contains no readable full-text passage on dendritic microtubule orientation; the full text appears only as page images, so the claim cannot be verified from admissible text. (LLM, conf=0.75)

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Cites

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Research works printed but not linked: 19

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Cited by

Sorted by citations

Authors
Baas, P. W., Deitch, J. S., Black, M. M., & Banker, G. A.
Affiliations
  • Temple University (US)
  • Albany Medical College (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1988-11
Volume
85
Issue
21
Pages
8335-8339
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.85.21.8335
PMC
PMC282424
OpenAlex
W1997122736
Cited by
742
References
1
Citations by year
2026: 17 · 2025: 14 · 2024: 11 · 2023: 20 · 2022: 35 · 2021: 28 · 2020: 37 · 2019: 22 · 2018: 28 · 2017: 34 · 2016: 37 · 2015: 28 · 2014: 17 · 2013: 26 · 2012: 23
FWCI
4.23
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS23530, NS 23094, NS 17681)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
microtubule polarity, hippocampal neurons, axons, dendrites, growth cones, microtubule organization, organelle distribution
MeSH terms
Animals, Axons, Dendrites, Hippocampus, Microscopy, Electron, Microscopy, Phase-Contrast, Microtubules, Neurons, Rats

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