The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
The requirement for lymphocyte function-associated antigen 1 in homotypic leukocyte adhesion stimulated by phorbol ester.
Open accessAbstract
Lymphocytes become adherent and aggregate after stimulation with phorbol esters such as PMA. Time-lapse video showed that aggregating cells were motile and exhibited vigorous pseudopodial movements. Adhesion sites were initiated between pseudopodia of neighboring cells, and then moved to the uropod. PMA-stimulated aggregation by EBV-transformed B cell lines, SKW-3 (a T cell line), differentiated U937 (a monocytic line), and blood lymphocytes was inhibited by mAbs to LFA-1. A number of different mAb to the LFA-1 alpha and beta subunits and F(ab')2 and Fab' fragments inhibited aggregation. Furthermore, lymphoblasts from normal individuals, but not from LFA-1-deficient patients, aggregated in response to PMA. These findings suggest LFA-1 is critically involved in stimulated lymphocyte adhesion. LFA-1 expression was not increased by PMA stimulation, showing that other mechanisms regulate LFA-1-dependent adherence. LFA-1-deficient patient cells were able to coaggregate with LFA-1+ cells, showing that aggregation is not mediated by like-like interactions between LFA-1 molecules on opposite cells. Aggregation was Mg+2-dependent, inhibited by cytochalasin B, and was reversed when LFA-1 mAb was added to preformed aggregates. Previous findings suggesting that LFA-1 is important in a wide variety of leukocyte functions are elucidated by this work, which shows that LFA-1 is a general leukocyte cell adhesion molecule, the activity of which is regulated by cell activation.
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Cites
Barnstable, C. (1978) · Cell · doi:10.1016/0092-8674(78)90296-9 · Cited by 1,916
Turnover of Inositol Phospholipids and Signal Transduction
Nishizuka, Y. (1984) · Science · doi:10.1126/science.6147898 · Cited by 1,614
Sanchez-Madrid, F., Nagy, J. A., Robbins, E., Simon, P., & Springer, T. A. (1983) · The Journal of experimental medicine · doi:10.1084/jem.158.6.1785 · Cited by 873
Self-citationSanchez-Madrid, F., Krensky, A. M., Ware, C. F., Robbins, E., Strominger, J. L., Burakoff, S. J., & Springer, T. A. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.23.7489 · Cited by 718
Self-citationAnderson, D. C., Schmalsteig, F. C., Finegold, M. J., Hughes, B. J., Rothlein, R., Miller, L. J., Kohl, S., Tosi, M. F., Jacobs, R. L., Waldrop, T. C., et al. (1985) · Journal of Infectious Diseases · doi:10.1093/infdis/152.4.668 · Cited by 634
Self-citationKrensky, A. M., Sanchez-Madrid, F., Robbins, E., Nagy, J. A., Springer, T. A., & Burakoff, S. J. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.131.2.611 · Cited by 571
Self-citationInherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis.
Springer, T. A., Thompson, W. S., Miller, L. J., Schmalstieg, F. C., & Anderson, D. C. (1984) · The Journal of experimental medicine · doi:10.1084/jem.160.6.1901 · Cited by 481
Self-citationKürzinger, K., Reynolds, T., Germain, R. N., Davignon, D., Martz, E., & Springer, T. A. (1981) · The Journal of Immunology · doi:10.4049/jimmunol.127.2.596 · Cited by 252
Self-citationPierres, M., Goridis, C., & Golstein, P. (1982) · European Journal of Immunology · doi:10.1002/eji.1830120112 · Cited by 237
LFA-1, LFA-2, and LFA-3 antigens are involved in CTL-target conjugation.
Krensky, A. M., Robbins, E. S., Springer, T. A., & Burakoff, S. J. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.132.5.2180 · Cited by 223
Self-citation
Cited by
Adhesion receptors of the immune system
Springer, T. A. (1990) · Nature · doi:10.1038/346425a0 · Cited by 6,477
Self-citationT-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1
Dustin, M. L., & Springer, T. A. (1989) · Nature · doi:10.1038/341619a0 · Cited by 1,538
Self-citationMetlay, J. P., Witmer-Pack, M. D., Agger, R., Crowley, M. T., Lawless, D., & Steinman, R. M. (1990) · The Journal of experimental medicine · doi:10.1084/jem.171.5.1753 · Cited by 555
Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., & Cabañas, C. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.19.11067 · Cited by 296
RhoH is required to maintain the integrin LFA-1 in a nonadhesive state on lymphocytes
Cherry, L. K., Li, X., Schwab, P., Lim, B., & Klickstein, L. B. (2004) · Nature Immunology · doi:10.1038/ni1103 · Cited by 76
Metadata
- Authors
- Rothlein, R., & Springer, T. A.
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1986-05-01
- Volume
- 163
- Issue
- 5
- Pages
- 1132-1149
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2157279574
- Cited by
- 474
- References
- 14
- FWCI
- 10.24
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rothlein, R., & Springer, T. A. (1986). The requirement for lymphocyte function-associated antigen 1 in homotypic leukocyte adhesion stimulated by phorbol ester. The Journal of experimental medicine. https://doi.org/10.1084/jem.163.5.1132
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