The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells
Open accessAbstract
The role of recombinant B cell stimulatory factor 2 (BSF-2/IL-6) in the regulation of growth and differentiation of B cells was investigated. rBSF-2 at 200 pg/ml could induce 50% of the maximum Ig production in B lymphoblastoid cell lines, the specific activity being estimated as 5 X 10(6) U/mg. rBSF-2 augmented PWM-induced IgM, IgG, and IgA production in mononuclear cells (MNC); the effect was exerted by directly acting on PWM-induced B blast cells to induce Ig production. However, rBSF-2 did not induce any growth of activated B cells. In contrast, rBSF-2 showed a potent growth activity on a murine hybridoma clone, MH60.BSF2. The concentration required for half-maximal [3H]TdR uptake was approximately 5 pg/ml, which was 40 times less than that required for Ig induction in a B cell line. Anti-BSF-2 antibody inhibited PWM-induced Ig production in MNC, but not PWM-induced proliferation. The antibody was effective even when added on day 4 of an 8-d culture, indicating that BSF-2 is one of the essential late-acting factors in PWM-induced Ig production.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
IL-6 promotes antibody production by directly acting on plasma cells and by indirectly promoting Bcl6-dependent follicular CD4+ T (Tfh) cell differentiation together with IL-21.
“Indeed, IL-6 promotes antibody production by directly acting on plasma cells and by indirectly promoting Bcl6-dependent follicular CD4(+) T (Tfh) cell differentiation together with IL-21 (Fig. 2) (143–146).”
From IL-6 in inflammation, autoimmunity and cancer (2021) · cites this paper as [143]
The paper’s summary reports that IL-6 (BSF-2) directly acts on PWM-induced B blast cells to induce immunoglobulin production, supporting the direct antibody-production component. It does not establish direct action on plasma cells or address Bcl6-dependent Tfh differentiation or cooperation with IL-21. (LLM, conf=0.87)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cited by
Sorted by citations
IL-6 in inflammation, autoimmunity and cancer
Hirano, T. (2021) · International Immunology · doi:10.1093/intimm/dxaa078 · Cited by 1,453 · see citing works
“2) (143–146).”
Self-citationAge-Associated Increased Interleukin-6 Gene Expression, Late-Life Diseases, and Frailty
Ershler, W. B., & Keller, E. T. (2000) · Annual Review of Medicine · doi:10.1146/annurev.med.51.1.245 · Cited by 1,282 · see citing works
Schindler, R., Mancilla, J., Endres, S., Ghorbani, R., Clark, S., & Dinarello, C. (1990) · Blood · doi:10.1182/blood.v75.1.40.40 · Cited by 1,012 · see citing works
Muscle‐derived interleukin‐6: possible biological effects
Pedersen, B. K., Steensberg, A., & Schjerling, P. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.0329c.xd · Cited by 599 · see citing works
Zhu, Z., Tang, W., Ray, A., Wu, Y., Einarsson, O., Landry, M. L., Gwaltney, J., & Elias, J. A. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118431 · Cited by 282 · see citing works
IL-6: Insights into Novel Biological Activities
Barton, B. E. (1997) · Clinical Immunology and Immunopathology · doi:10.1006/clin.1997.4420 · Cited by 269 · see citing works
Frei, K., Leist, T. P., Meager, A., Gallo, P., Leppert, D., Zinkernagel, R. M., & Fontana, A. (1988) · The Journal of experimental medicine · doi:10.1084/jem.168.1.449 · Cited by 245 · see citing works
Inflammation and age-associated skeletal muscle deterioration (sarcopaenia)
Wang, J., Leung, K.-S., Chow, S. K.-H., & Cheung, W.-H. (2017) · Journal of Orthopaedic Translation · doi:10.1016/j.jot.2017.05.006 · Cited by 173 · see citing works
Crnkic Kapetanovic, M., Saxne, T., Jönsson, G., Truedsson, L., & Geborek, P. (2013) · Arthritis Research & Therapy · doi:10.1186/ar4358 · Cited by 142 · see citing works
Katsume, A., Saito, H., Yamada, Y., Yorozu, K., Ueda, O., Akamatsu, K.-I., Nishimoto, N., Kishimoto, T., Yoshizaki, K., & Ohsugi, Y. (2002) · Cytokine · doi:10.1006/cyto.2002.2012 · Cited by 134 · see citing works
Self-citationRegulation of IgE synthesis in humans
Geha, R. S. (1992) · Journal of Allergy and Clinical Immunology · doi:10.1016/0091-6749(92)90064-9 · Cited by 87 · see citing works
Hirano, T., & Kishimoto, T. (1990) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-642-49295-2_14 · Cited by 57 · see citing works
Self-citation
- Authors
- Muraguchi, A., Hirano, T., Tang, B., Matsuda, T., Horii, Y., Nakajima, K., & Kishimoto, T.
- Affiliations
- The University of Osaka (JP)
- Venue
- The Journal of experimental medicine
- Publisher
- Rockefeller University Press
- Published
- 1988-02-01
- Volume
- 167
- Issue
- 2
- Pages
- 332-344
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2188837
- OpenAlex
- W2168822788
- Cited by
- 783
- References
- 0
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 11 · 2023: 14 · 2022: 14 · 2021: 10 · 2020: 13 · 2019: 12 · 2018: 4 · 2017: 13 · 2016: 13 · 2015: 7 · 2014: 15 · 2013: 18 · 2012: 15
- FWCI
- 31.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Keywords
- B cell stimulatory factor 2, interleukin-6, B cell terminal differentiation, antibody production, B-lymphoblastoid cell lines, B cell proliferation, mononuclear cells
- MeSH terms
- Antibodies, B-Lymphocytes, Cell Differentiation, Cell Line, Transformed, Humans, Hybridomas, Immunoglobulins, Interleukins, Lymphocyte Activation, Pokeweed Mitogens, Recombinant Proteins, Interleukin-4
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
