The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Alveolar Macrophage–mediated Killing of Pneumocystis carinii f. sp. muris Involves Molecular Recognition by the Dectin-1 β-Glucan Receptor
Open accessAbstract
Innate immune mechanisms against Pneumocystis carinii, a frequent cause of pneumonia in immunocompromised individuals, are not well understood. Using both real time polymerase chain reaction as a measure of organism viability and fluorescent deconvolution microscopy, we show that nonopsonic phagocytosis of P. carinii by alveolar macrophages is mediated by the Dectin-1 β-glucan receptor and that the subsequent generation of hydrogen peroxide is involved in alveolar macrophage–mediated killing of P. carinii. The macrophage Dectin-1 β-glucan receptor colocalized with the P. carinii cyst wall. However, blockage of Dectin-1 with high concentrations of anti–Dectin-1 antibody inhibited binding and concomitant killing of P. carinii by alveolar macrophages. Furthermore, RAW 264.7 macrophages overexpressing Dectin-1 bound P. carinii at a higher level than control RAW cells. In the presence of Dectin-1 blockage, killing of opsonized P. carinii could be restored through FcγRII/III receptors. Opsonized P. carinii could also be efficiently killed in the presence of FcγRII/III receptor blockage through Dectin-1–mediated phagocytosis. We further show that Dectin-1 is required for P. carinii–induced macrophage inflammatory protein 2 production by alveolar macrophages. Taken together, these results show that nonopsonic phagocytosis and subsequent killing of P. carinii by alveolar macrophages is dependent upon recognition by the Dectin-1 β-glucan receptor.
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Cites
Brown, G. D., & Gordon, S. (2001) · Nature · doi:10.1038/35092620 · Cited by 1,579
Self-citationKilling activity of neutrophils is mediated through activation of proteases by K+ flux
Reeves, E. P., Lu, H., Jacobs, H. L., Messina, C. G. M., Bolsover, S., Gabella, G., Potma, E. O., Warley, A., Roes, J., & Segal, A. W. (2002) · Nature · doi:10.1038/416291a · Cited by 1,153
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Brown, G. D. (2006) · Nature Reviews Immunology · doi:10.1038/nri1745 · Cited by 1,158
Self-citationAn integrated model of the recognition of Candida albicans by the innate immune system
Netea, M. G., Brown, G. D., Kullberg, B. J., & Gow, N. A. R. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1815 · Cited by 946
Self-citationThe Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus
Steele, C., Rapaka, R. R., Metz, A., Pop, S. M., Williams, D. L., Gordon, S., Kolls, J. K., & Brown, G. D. (2005) · PLoS Pathogens · doi:10.1371/journal.ppat.0010042 · Cited by 509
Self-citationAspergillus fumigatus Triggers Inflammatory Responses by Stage-Specific β-Glucan Display
Hohl, T. M., Van Epps, H. L., Rivera, A., Morgan, L. A., Chen, P. L., Feldmesser, M., & Pamer, E. G. (2005) · PLoS Pathogens · doi:10.1371/journal.ppat.0010030 · Cited by 428
Immune responses to human fungal pathogens and therapeutic prospects
Lionakis, M. S., Drummond, R. A., & Hohl, T. M. (2023) · Nature Reviews Immunology · doi:10.1038/s41577-022-00826-w · Cited by 379
Gersuk, G. M., Underhill, D. M., Zhu, L., & Marr, K. A. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.6.3717 · Cited by 334
Aspergillus fumigatus : Principles of Pathogenesis and Host Defense
Hohl, T. M., & Feldmesser, M. (2007) · Eukaryotic Cell · doi:10.1128/ec.00274-07 · Cited by 237
Munro, C. A., Bates, S., Buurman, E. T., Hughes, H. B., MacCallum, D. M., Bertram, G., Atrih, A., Ferguson, M. A. J., Bain, J. M., Brand, A., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m411413200 · Cited by 200
Zhu, D., Pan, C., Li, L., Bian, Z., Lv, Z., Shi, L., Zhang, J., Li, D., Gu, H., Zhang, C.-Y., et al. (2013) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2013.02.005 · Cited by 140
Development and Functions of Alveolar Macrophages
Woo, Y. D., Jeong, D., & Chung, D. H. (2021) · Molecules and Cells · doi:10.14348/molcells.2021.0058 · Cited by 110
Metadata
- Authors
- Steele, C., Marrero, L., Swain, S., Harmsen, A. G., Zheng, M., Brown, G. D., Gordon, S., Shellito, J. E., & Kolls, J. K.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2003-12-01
- Volume
- 198
- Issue
- 11
- Pages
- 1677-1688
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2101286028
- Cited by
- 289
- References
- 2
- FWCI
- 12.33
- Type
- journal article
- Language
- en
- Open access
- bronze
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