The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Antigen-loaded MR1 tetramers define T cell receptor heterogeneity in mucosal-associated invariant T cells
Open accessAbstract
Mucosal-associated invariant T cells (MAIT cells) express a semi-invariant T cell receptor (TCR) α-chain, TRAV1-2–TRAJ33, and are activated by vitamin B metabolites bound by the major histocompatibility complex (MHC)–related class I–like molecule, MR1. Understanding MAIT cell biology has been restrained by the lack of reagents to specifically identify and characterize these cells. Furthermore, the use of surrogate markers may misrepresent the MAIT cell population. We show that modified human MR1 tetramers loaded with the potent MAIT cell ligand, reduced 6-hydroxymethyl-8-d-ribityllumazine (rRL-6-CH2OH), specifically detect all human MAIT cells. Tetramer+ MAIT subsets were predominantly CD8+ or CD4−CD8−, although a small subset of CD4+ MAIT cells was also detected. Notably, most human CD8+ MAIT cells were CD8α+CD8β−/lo, implying predominant expression of CD8αα homodimers. Tetramer-sorted MAIT cells displayed a TH1 cytokine phenotype upon antigen-specific activation. Similarly, mouse MR1–rRL-6-CH2OH tetramers detected CD4+, CD4−CD8− and CD8+ MAIT cells in Vα19 transgenic mice. Both human and mouse MAIT cells expressed a broad TCR-β repertoire, and although the majority of human MAIT cells expressed TRAV1-2–TRAJ33, some expressed TRAJ12 or TRAJ20 genes in conjunction with TRAV1-2. Accordingly, MR1 tetramers allow precise phenotypic characterization of human and mouse MAIT cells and revealed unanticipated TCR heterogeneity in this population.
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Cites
Phenotypic Analysis of Antigen-Specific T Lymphocytes
Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M. (1996) · Science · doi:10.1126/science.274.5284.94 · Cited by 3,544 · cited 1×
“Tetramers of Agpresenting molecules permit Ag-specific T cells to be isolated, quantified, tracked, and characterized from the milieu of T cells within the host (Altman et al., 1996; Davis et al., 2011).”
BackgroundMR1 presents microbial vitamin B metabolites to MAIT cells
Kjer-Nielsen, L., Patel, O., Corbett, A. J., Le Nours, J., Meehan, B., Liu, L., Bhati, M., Chen, Z., Kostenko, L., Reantragoon, R., et al. (2012) · Nature · doi:10.1038/nature11605 · Cited by 1,351
Self-citationSelection of evolutionarily conserved mucosal-associated invariant T cells by MR1
Treiner, E., Duban, L., Bahram, S., Radosavljevic, M., Wanner, V., Tilloy, F., Affaticati, P., Gilfillan, S., & Lantz, O. (2003) · Nature · doi:10.1038/nature01433 · Cited by 1,139
Wang, G. C., Dash, P., McCullers, J. A., Doherty, P. C., & Thomas, P. G. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003647 · Cited by 238
Interrogating the repertoire: broadening the scope of peptide–MHC multimer analysis
Davis, M. M., Altman, J. D., & Newell, E. W. (2011) · Nature Reviews Immunology · doi:10.1038/nri3020 · Cited by 124
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531
Self-citationImpairment of immunity to Candida and Mycobacterium in humans with bi-allelic RORC mutations
Okada, S., Markle, J. G., Deenick, E. K., Mele, F., Averbuch, D., Lagos, M., Alzahrani, M., Al-Muhsen, S., Halwani, R., Ma, C. S., et al. (2015) · Science · doi:10.1126/science.aaa4282 · Cited by 413
Self-citationA three-stage intrathymic development pathway for the mucosal-associated invariant T cell lineage
Koay, H.-F., Gherardin, N. A., Enders, A., Loh, L., Mackay, L. K., Almeida, C. F., Russ, B. E., Nold-Petry, C. A., Nold, M. F., Bedoui, S., et al. (2016) · Nature Immunology · doi:10.1038/ni.3565 · Cited by 377
Self-citationSingh, M., Al-Eryani, G., Carswell, S., Ferguson, J. M., Blackburn, J., Barton, K., Roden, D., Luciani, F., Giang Phan, T., Junankar, S., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11049-4 · Cited by 308
Metadata
- Authors
- Reantragoon, R., Corbett, A. J., Sakala, I. G., Gherardin, N. A., Furness, J. B., Chen, Z., Eckle, S. B. G., Uldrich, A. P., Birkinshaw, R. W., Patel, O., Kostenko, L., Meehan, B., Kedzierska, K., Liu, L., Fairlie, D. P., Hansen, T. H., Godfrey, D. I., Rossjohn, J., McCluskey, J., & Kjer-Nielsen, L.
- Venue
- Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2013-10-07
- Volume
- 210
- Issue
- 11
- Pages
- 2305-2320
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2151505523
- Cited by
- 589
- References
- 5
- FWCI
- 18.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Reantragoon, R., Corbett, A. J., Sakala, I. G., Gherardin, N. A., Furness, J. B., Chen, Z., Eckle, S. B. G., Uldrich, A. P., Birkinshaw, R. W., Patel, O., Kostenko, L., Meehan, B., Kedzierska, K., Liu, L., Fairlie, D. P., Hansen, T. H., Godfrey, D. I., Rossjohn, J., McCluskey, J., & Kjer-Nielsen, L. (2013). Antigen-loaded MR1 tetramers define T cell receptor heterogeneity in mucosal-associated invariant T cells. Journal of Experimental Medicine. https://doi.org/10.1084/jem.20130958
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