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Identification of In Vivo–Expressed Antigens of Staphylococcus aureus and Their Use in Vaccinations for Protection against Nasal Carriage
Abstract
A spectrum of in vivo-expressed Staphylococcus aureus antigens was identified by probing bacteriophage expression libraries of S. aureus with serum samples from infected and uninfected individuals. Eleven recombinant antigenic proteins were produced, and specific antibody titers in a large collection of human serum samples were determined. Significantly increased concentrations of reactive immunoglobulin G (IgG) to 7 antigens were found in serum samples from ill individuals, compared with those in healthy individuals. Significantly higher concentrations of reactive IgG to 4 antigens, including iron-responsive surface determinant (Isd) A and IsdH, were found in serum samples from healthy individuals who were not nasal carriers of S. aureus, compared with those in healthy carriers. Vaccination of cotton rats with IsdA or IsdH protected against nasal carriage. Also, IsdA is involved in adherence of S. aureus to human desquamated nasal epithelial cells and is required for nasal colonization in the cotton rat model. Thus, vaccination with these antigens may prevent S. aureus carriage and reduce the prevalence of human disease.
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“Consistent with this hypothesis, IsdA facilitates binding to human desquamated epithelial cells, and promotes nasal colonization in rats [122, 126, 127].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [122]
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“IsdA and IsdB were identified as vaccine candidates using “reverse vaccinology” [119], and subsequent studies confirmed that immunization with these proteins is protective in murine models of infection [117, 120–123].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [122]
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Metadata
- Authors
- Clarke, S. R., Brummell, K. J., Horsburgh, M. J., McDowell, P. W., Mohamad, S. A. S., Stapleton, M. R., Acevedo, J., Read, R. C., Day, N. P. J., Peacock, S. J., Mond, J. J., Kokai‐Kun, J. F., & Foster, S. J.
- Affiliations
- University of Sheffield (GB)
- Mahidol University (TH)
- ORCID
- Venue
- The Journal of Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-04-15
- Volume
- 193
- Issue
- 8
- Pages
- 1098-1108
- ISSN
- 0022-1899 (ISSN-L)
- 1537-6613
- OpenAlex
- W2103675658
- Cited by
- 208
- References
- 49
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 4 · 2022: 4 · 2021: 1 · 2020: 6 · 2019: 9 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 19 · 2014: 8 · 2013: 12 · 2012: 16
- FWCI
- 9.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Vanderbilt University
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- Staphylococcus aureus, nasal carriage, bacterial antigens, IgG, ISDA, IsdH, vaccine development, bacterial adhesion, cotton rat model, antibody titers
- MeSH terms
- Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Vaccines, Carrier State, Cells, Cultured, Epithelial Cells, Female, Humans, Immunoglobulin G, Nasal Cavity, Receptors, Cell Surface, Recombinant Proteins, Staphylococcal Infections, Staphylococcus aureus, Sigmodontinae, DNA Primers, Cation Transport Proteins
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