Biocompatibility of Fe3O4nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells
Abstract
In order to reveal the biocompatibility of Fe(3)O(4) nanoparticles and bipolar surfactant tetramethylammonium 11-aminoundecanoate cytotoxicity tests were performed as a function of concentration from low (0.1 microg ml(-1)) to higher concentration (100 microg ml(-1)) using various human glia, human breast cancer and normal cell lines. Cytotoxicity tests for human glia (D54MG, G9T, SF126, U87, U251, U373), human breast cancer (MB157, SKBR3, T47D) and normal (H184B5F5/M10, WI-38, SVGp12) cell lines exhibited almost nontoxicity and reveal biocompatibility of Fe(3)O(4) nanoparticles in the concentration range of 0.1-10 microg ml(-1), while accountable cytotoxicity can be seen at 100 microg ml(-1). The results of our studies suggest that Fe(3)O(4) nanoparticles coated with bipolar surfactant tetramethylammonium 11-aminoundecanoate are biocompatible and promising for bio-applications such as drug delivery, magnetic resonance imaging and magnetic hyperthermia.
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In vitro, upon prolonged incubation, surface-coated SPION are typically nontoxic at iron concentrations up to 10 μg/ml, while slight cytotoxicity is seen at 100 μg/ml.
“In vitro, upon prolonged incubation, surface-coated SPION are typically nontoxic at iron concentration up to and 10 μg/ml, while slight cytotoxicity is seen at 100 μg/ml.”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [119]
SPION toxicity depends on dosing, exposure time, nature of the coating material, hydrodynamic diameter of the particle and SPION-protein interactions.
“In general, SPION toxicity depends on a variety of factors, including - besides dosing and exposure time - also the nature of the coating material, the hydrodynamic diameter of the particle and SPION-protein interactions [119–122].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [119]
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- Authors
- Ankamwar, B., Lai, T. C., Huang, J. H., Liu, R. S., Hsiao, M., Chen, C. H., & Hwu, Y. K.
- Affiliations
- National Taiwan University (TW)
- Genomics Research Center, Academia Sinica (TW)
- Institute of Physics, Academia Sinica (TW)
- Corresponding author
- Liu, R S
- Venue
- Nanotechnology
- Publisher
- IOP Publishing
- Published
- 2010-01-21
- Volume
- 21
- Issue
- 7
- Pages
- 075102
- ISSN
- 0957-4484 (ISSN-L)
- 1361-6528
- OpenAlex
- W2073884591
- Cited by
- 267
- References
- 42
- Citations by year
- 2026: 1 · 2025: 14 · 2024: 15 · 2023: 15 · 2022: 17 · 2021: 29 · 2020: 17 · 2019: 23 · 2018: 21 · 2017: 18 · 2016: 20 · 2015: 20 · 2014: 21 · 2013: 16 · 2012: 11
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- Fe3O4 nanoparticles, magnetic nanoparticles, biocompatibility, cytotoxicity, glial cells, breast cancer cells, surfactant coating, drug delivery, magnetic hyperthermia, magnetic resonance imaging
- MeSH terms
- Amino Acids, Quaternary Ammonium Compounds, Biocompatible Materials, Breast, Breast Neoplasms, Cell Line, Cell Survival, Female, Ferric Compounds, Humans, Materials Testing, Microscopy, Neuroglia, Surface-Active Agents, Cell Line, Tumor, Metal Nanoparticles
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