In vitro and in vivo experiments with iron oxide nanoparticles functionalized with DEXTRAN or polyethylene glycol for medical applications: Magnetic targeting
Open accessAbstract
In this research work, DEXTRAN- and polyethylene glycol (PEG)-coated iron-oxide superparamagnetic nanoparticles were synthetized and their cytotoxicity and biodistribution assessed. Well-crystalline hydrophobic Fe3 O4 SPIONs were formed by a thermal decomposition process with d = 18 nm and σ = 2 nm; finally, the character of SPIONs was changed to hydrophilic by a post-synthesis procedure with the functionalization of the SPIONs with PEG or DEXTRAN. The nanoparticles present high saturation magnetization and superparamagnetic behavior at room temperature, and the hydrodynamic diameters of DEXTRAN- and PEG-coated SPIONs were measured as 170 and 120 nm, respectively. PEG- and DEXTRAN-coated SPIONs have a Specific Power Absorption SPA of 320 and 400 W/g, respectively, in an ac magnetic field with amplitude of 13 kA/m and frequency of 256 kHz. In vitro studies using VERO and MDCK cell lineages were performed to study the cytotoxicity and cell uptake of the SPIONs. For both cell lineages, PEG- and DEXTRAN-coated nanoparticles presented high cell viability for concentrations as high as 200 μg/mL. In vivo studies were conducted using BALB/c mice inoculating the SPIONs intravenously and exposing them to the presence of an external magnet located over the tumour. It was observed that the amount of PEG-coated SPIONs in the tumor increased by up to 160% when using the external permanent magnetic as opposed to those animals that were not exposed to the external magnetic field.
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Among different polymer coatings, PEG and dextran are the most extensively employed, likely because they are generally regarded as safe and are not very rapidly recognized by macrophages in liver and spleen when administered intravenously.
“Among the different polymer coatings, PEG and dextran are the most extensively employed ones, likely because they are generally regarded as safe (GRAS), and also because they are not very rapidly recognized by macrophages in liver and spleen when administered intravenously [85, 86].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [85]
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Research works printed but not linked: 1
- Ankamwar B, Lai TC, Huang JH, Liu RS, Hsiao M, Chen CH, Hwu YK. Biocompatibility of Fe3O4 nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells. Nanotechnology 2010;21:075102.
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Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications
Dadfar, S. M., Roemhild, K., Drude, N. I., von Stillfried, S., Knüchel, R., Kiessling, F., & Lammers, T. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.01.005 · Cited by 1,181 · see citing works
Remya, N. S., Syama, S., Sabareeswaran, A., & Mohanan, P. V. (2016) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2016.06.119 · Cited by 97 · see citing works
- Authors
- Mojica Pisciotti, M. L., Lima, E., Vasquez Mansilla, M., Tognoli, V. E., Troiani, H. E., Pasa, A. A., Creczynski‐Pasa, T. B., Silva, A. H., Gurman, P., Colombo, L., Goya, G. F., Lamagna, A., & Zysler, R. D.
- Affiliations
- Div. Resonancias Magnéticas, Centro Atómico Bariloche/CONICET S. C. Bariloche 8400 Argentina
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidade Federal de Santa Catarina (BR)
- Centro Atómico Constituyentes (AR)
- Universidad de Zaragoza (ES)
- Corresponding author
- Lima, E.
- Venue
- Journal of Biomedical Materials Research Part B Applied Biomaterials
- Publisher
- Wiley
- Published
- 2014-01-23
- Volume
- 102
- Issue
- 4
- Pages
- 860-868
- ISSN
- 1552-4973 (ISSN-L)
- 1552-4981
- OpenAlex
- W2097338689
- Cited by
- 87
- References
- 49
- Citations by year
- 2025: 4 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 8 · 2020: 15 · 2019: 8 · 2018: 7 · 2017: 7 · 2016: 8 · 2015: 5 · 2014: 5
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Biomedical Materials Research Part B Applied Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
- Funders
- Comisión Nacional de Energía Atómica, Gobierno de Argentina
- Agencia Nacional de Promoción Científica y Tecnológica
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Materials Science › Materials Chemistry › Magnetic Properties and Synthesis of Ferrites
- Keywords
- iron oxide nanoparticles, superparamagnetic iron oxide nanoparticles, dextran coating, polyethylene glycol coating, magnetic targeting, thermal decomposition synthesis, cytotoxicity, tissue distribution, cellular uptake, external magnetic field, tumor accumulation, magnetic hyperthermia, Vero cells, MDCK cells
- MeSH terms
- Animals, Chlorocebus aethiops, Dextrans, Dogs, Drug Carriers, Drug Evaluation, Preclinical, Female, Ferric Compounds, Injections, Intravenous, Liver, Lung, Mammary Neoplasms, Experimental, Materials Testing, Mice, Inbred BALB C, Polyethylene Glycols, Skin, Tissue Distribution, Vero Cells, Spectroscopy, Fourier Transform Infrared, Mice, Nanoparticles, Magnetite Nanoparticles, Magnetic Fields, Madin Darby Canine Kidney Cells, In Vitro Techniques
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