Angiogenic potential of human macrophages on electrospun bioresorbable vascular grafts
Open accessAbstract
The aim of this study was to investigate macrophage interactions with electrospun scaffolds and quantify the expression of key angiogenic growth factors in vitro. This study will further help in evaluating the potential of these electrospun constructs as vascular grafts for tissue repair and regeneration in situ. Human peripheral blood macrophages were seeded in serum free media on electrospun (10 mm) discs of polydioxanone (PDO), elastin and PDO:elastin blends (50:50, 70:30 and 90:10). The growth factor secretion was analyzed by ELISA. Macrophages produced high levels of vascular endothelial growth factor and acidic fibroblast growth factor. Transforming growth factor beta-1 (TGF-beta1) secretion was relatively low and there was negligible production of basic fibroblast growth factor. Therefore, it can be anticipated that these scaffolds will support tissue regeneration and angiogenesis.
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PDO was chosen because electrospinning at a particular concentration produces scaffolds with a fairly uniform distribution of fiber sizes and pore sizes.
“PDO was chosen for this study because when electrospun at a particular concentration, it produces scaffolds with a fairly uniform distribution of fiber sizes and pore sizes [18, 20].”
From Macrophage functional polarization (M1/M2) in response to varying fiber and pore dimensions of electrospun scaffolds (2013) · cites this paper as [20]
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Biomaterials: Foreign Bodies or Tuners for the Immune Response?
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Self-citation
- Authors
- Garg, K., Sell, S. A., Madurantakam, P., & Bowlin, G. L.
- Affiliations
- Virginia Commonwealth University (US)
- Venue
- Biomedical Materials
- Publisher
- IOP Publishing
- Published
- 2009-04-17
- Volume
- 4
- Issue
- 3
- Pages
- 031001
- ISSN
- 1748-6041 (ISSN-L)
- 1748-605X
- OpenAlex
- W1977217812
- Cited by
- 48
- References
- 42
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 7 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 5
- FWCI
- 1.75
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Biomedical Materials (publishedVersion)
- PubMed (publishedVersion)
- VCU Scholars Compass (Virginia Commonwealth University) (submittedVersion)
- VCULIBRARIES
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- macrophages, angiogenesis, electrospun scaffolds, polydioxanone, elastin, vascular endothelial growth factor, acidic fibroblast growth factor, TGF-β1, basic fibroblast growth factor, growth factor secretion, tissue regeneration
- MeSH terms
- Biocompatible Materials, Bioprosthesis, Blood Vessel Prosthesis, Cells, Cultured, Elastin, Electrochemistry, Humans, Macrophages, Materials Testing, Rotation, Polydioxanone, Neovascularization, Physiologic, Absorbable Implants, Tissue Engineering, Angiogenic Proteins, Transforming Growth Factor beta1
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