The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
In Vitro and in Vivo Degradation of Mineralized Collagen-Based Composite Scaffold: Nanohydroxyapatite/Collagen/Poly(L-lactide)
Abstract
The objective of this article was to investigate the in vitro and in vivo biodegradation of a novel biomimetic bone scaffold composite, nanohydroxyapatite/collagen/poly(L-lactide), that could be used for bone tissue engineering. For evaluation of in vitro degradation specimens were immersed into 1% trypsin/phosphate-buffered saline solution at 37 degrees C. In vivo evaluation involved the implantation of samples into the posterolateral lumbar spine of rabbits, and the retrieved specimens were analyzed by Fourier transform-infrared spectroscopy. The results demonstrated that weight loss increased continuously in vitro with a reduction in mass of 19.6% after 4 weeks. During the experimental period in vitro, the relative rate of reduction of the three components in this material was shown to differ greatly: collagen decreased the fastest, from 40% by weight to 20% in the composite; hydroxyapatite content increased from 45 to 60%; and PLA changed little. The pore structure was maintained throughout the whole experimental period in vitro; however, the thickness of the walls of the pores decreased and the surface of the walls increased in roughness. In vivo, the ratio of collagen to hydroxyapatite appeared to be slightly higher near the transverse process than in the central part of the intertransverse process. This finding may have been due to new bone matrix formation extending from the transverse to the intertransverse process.
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In the citing paper
“In the invivo condition, there were no appropriate quantitative data regarding the degradation rate”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“Liao et al [87] studied the in-vitro (1% trypsin/phosphatebuffered saline solution) and in-vivo (implanted in the posterolateral lumbar spine of rabbits) biodegradation of nano-HA, collagen, and poly(L-lactide) (PLLA) scaffold fabricated through freeze drying process.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“approximate measurements showed that the in-vivo degradation was faster than in-vitro.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“However, after 28 d the pore wall thickness declined to 2 µm substantiating the degradation process.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“Even though the pore thickness has decreased, the pores along with pore walls were still visible.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“After 4 weeks, there was a 19.6% weight loss due to the degradation of the scaffold material.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“From the SEM images of the scaffold after 10 d of in-vitro treatment, the porous structures were found to be intact with a wall thickness of about 10 µm.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“Due to the presence of collagenase inside the body, collagen degrades rapidly and it is difficult to monitor the degradation behavior of collagen in vivo [87,122].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
In the citing paper
“Due to the presence of collagenase inside the body, collagen degrades rapidly and it is difficult to monitor the degradation behavior of collagen in vivo [87,122].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [87]
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Cited by
Sorted by citations
Hutmacher, D. W., Schantz, J. T., Lam, C. X. F., Tan, K. C., & Lim, T. C. (2007) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.24 · Cited by 1,007
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Self-citationCalcium orthophosphate-based biocomposites and hybrid biomaterials
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Metadata
- Authors
- Liao, S. S., & Cui, F. Z.
- Affiliations
- Tsinghua University (CN)
- ORCID
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2004-01
- Volume
- 10
- Issue
- 1-2
- Pages
- 73-80
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W1996507095
- Cited by
- 142
- References
- 27
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 8 · 2019: 2 · 2018: 2 · 2017: 6 · 2016: 13 · 2015: 15 · 2014: 7 · 2013: 4 · 2012: 10
- FWCI
- 6.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Tissue Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- biodegradation, hydroxyapatite nanoparticles, collagen, poly(L-lactic acid), bone scaffolds, bone tissue engineering, trypsin degradation, in vivo implantation, lumbar spine, new bone formation, pore structure, weight loss
- MeSH terms
- Animals, Collagen, Hydrogen-Ion Concentration, Lumbar Vertebrae, Polyesters, Rabbits, Temperature, Time Factors, Spectroscopy, Fourier Transform Infrared, Durapatite, Bone Substitutes, Prostheses and Implants, In Vitro Techniques
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- CoChat. (2026). Citation check: In Vitro and in Vivo Degradation of Mineralized Collagen-Based Composite Scaffold: Nanohydroxyapatite/Collagen/Poly(L-lactide). https://app.cochat.ai/papers/10.1089/107632704322791718
- The paper
- Liao, S. S., & Cui, F. Z. (2004). In Vitro and in Vivo Degradation of Mineralized Collagen-Based Composite Scaffold: Nanohydroxyapatite/Collagen/Poly(L-lactide). Tissue Engineering. https://doi.org/10.1089/107632704322791718
