The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Analysis of Thermal Melting Curves
Abstract
T(m) is defined as Temperature of melting or, more accurately, as temperature of midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and polynucleotides). A thermal denaturation experiment determines the stability of the secondary structure of a DNA or RNA and aids in the choice of the sequences for antisense oligomers or PCR primers. Beyond a simple numerical value (the T(m)), a thermal denaturation experiment, in which the folded fraction of a structure is plotted vs. temperature, yields important thermodynamic information. We present the classic problems encountered during these experiments and try to demonstrate that a number of useful pieces of information can be extracted from these experimental curves.
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Citations
Cites
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
Mathews, D. H., Sabina, J., Zuker, M., & Turner, D. H. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.2700 · Cited by 3,795
Predicting DNA duplex stability from the base sequence
Breslauer, K. J., Frank, R., Blöcker, H., & Marky, L. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.11.3746 · Cited by 1,837
Improved free-energy parameters for predictions of RNA duplex stability.
Freier, S. M., Kierzek, R., Jaeger, J. A., Sugimoto, N., Caruthers, M. H., Neilson, T., & Turner, D. H. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.24.9373 · Cited by 1,470
Hall, K. B., & McLaughlin, L. W. (1991) · Biochemistry · doi:10.1021/bi00108a002 · Cited by 112
Cited by
An RNA G-quadruplex in the 5′ UTR of the NRAS proto-oncogene modulates translation
Kumari, S., Bugaut, A., Huppert, J. L., & Balasubramanian, S. (2007) · Nature Chemical Biology · doi:10.1038/nchembio864 · Cited by 822
Thermal difference spectra: a specific signature for nucleic acid structures
Mergny, J.-L. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni134 · Cited by 469
Self-citationFluorescence-based melting assays for studying quadruplex ligands
DECIAN, A., GUITTAT, L., KAISER, M., SACCA, B., AMRANE, S., BOURDONCLE, A., ALBERTI, P., TEULADEFICHOU, M., LACROIX, L., & MERGNY, J. (2007) · Methods · doi:10.1016/j.ymeth.2006.10.004 · Cited by 381
Self-citationRescifina, A., Zagni, C., Varrica, M. G., Pistarà, V., & Corsaro, A. (2014) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2013.11.029 · Cited by 274
Li, X., Peng, Y., Ren, J., & Qu, X. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0607245103 · Cited by 273
Metadata
- Authors
- Mergny, J.-L., & Lacroix, L.
- Venue
- Oligonucleotides
- Publisher
- SAGE Publications
- Published
- 2003-12
- Volume
- 13
- Issue
- 6
- Pages
- 515-537
- ISSN
- 1545-4576 (ISSN-L)
- 1557-8526
- OpenAlex
- W2109887615
- Cited by
- 768
- References
- 5
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- closed
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