Effect of Trasina ® , an Ayurvedic Herbal Formulation, on Experimental Models of Alzheimer's Disease and Central Cholinergic Markers in Rats
Abstract
Trasina is a herbal formulation of some Indian medicinal plants classified in Ayurveda, the classic Indian system of medicine, as Medhyarasayanas or drugs reputed to improve memory and intellect. Earlier experimental and clinical investigations have indicated that the formulation has a memory-facilitating action. In this investigation, the effect of Trasina, after subchronic administration for 21 days, was assessed on two rodent models simulating some biochemical features known to be associated with Alzheimer's disease (AD). The models, in rats, included intracerebroventricularly (i.c.v.) administered colchicine (15 micrograms/rat) and lesioning of nucleus basalis magnocellularis (nbm) by ibotenic acid (10 micrograms/rat). Retention of an active avoidance response was used as the memory parameter. In addition, the effect of Trasina was evaluated on i.c.v. colchicine-induced depletion of acetylcholine (ACh) concentrations, reduction in choline acetyltransferase (ChAT) activity, and decrease in muscarinic cholinergic receptor (MCR) binding in rat brain frontal cortex and hippocampus. The behavioral and biochemical investigations were done 7, 14, and 21 days after colchicine or ibotenic acid lesioning. Trasina (200 and 500 mg/kg) was administered orally (p.o.) once daily for 21 days, the first drug administration being given just prior to lesioning. Colchicine and ibotenic acid induced marked retention deficit of active avoidance learning that was attenuated in a dose-dependent manner by Trasina after 14 and 21 days of treatment. Frontal cortical and hippocampal ACh concentrations, ChAT activity and MCR binding was significantly reduced after colchicine treatment. Trasina (200 and 500 mg/kg) reversed these deficits after 14 and 21 days of treatment. The findings indicate that the herbal formulation exerts a significant nootropic effect after subchronic treatment that may be due to reversal of perturbed cholinergic function.
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Trasina dose dependently improved both memory and cholinergic markers like acetylcholine concentration, choline acetyl transferase activity and muscarinic cholinergic receptor binding in the frontal cortex and hippocampus of rat brain after 14 and 21 days of treatment.
“Trasina, dose dependently, improved both memory and cholinergic markers like acetylcholine concentration, choline acetyl transferase activity and muscarinic cholinergic receptor binding in the frontal cortex and hippocampus of rat brain after 14 and 21 days of treatment.”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [255]
Trasina's nootropic effect may be attributed to the correction of cholinergic dysfunction.
“Thus, its nootropic effect may be attributed to the correction of cholinergic dysfunction [255].”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [255]
Trasina has shown significant nootropic effect at a dose of 200 and 500 mg/kg, p.o when administered for 21 days in colchicine and ibotenic acid induced cognitive impairment.
“It has shown significant nootropic effect at a dose of 200 and 500 mg/kg, p.o when administered for 21 days in colchicine and ibotenic acid induced cognitive impairment.”
From Indian Medicinal Herbs and Formulations for Alzheimer’s Disease, from Traditional Knowledge to Scientific Assessment (2020) · cites this paper as [255]
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- Authors
- Bhattacharya, S. K., & Kumar, A.
- Affiliations
- Banaras Hindu University (IN)
- Leipzig University (DE)
- ORCID
- Kumar, A.
- Venue
- The Journal of Alternative and Complementary Medicine
- Publisher
- SAGE Publications
- Published
- 1997-12
- Volume
- 3
- Issue
- 4
- Pages
- 327-336
- ISSN
- 1075-5535 (ISSN-L)
- 1557-7708
- OpenAlex
- W1965799648
- Cited by
- 32
- References
- 33
- Citations by year
- 2025: 1 · 2023: 1 · 2021: 2 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Alternative and Complementary Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Keywords
- Ayurvedic herbal formulation, Alzheimer's disease, cholinergic system, acetylcholine depletion, choline acetyltransferase, muscarinic receptor binding, active avoidance, memory impairment, nootropic effects, frontal cortex, hippocampus
- MeSH terms
- Acetylcholine, Alzheimer Disease, Animals, Binding Sites, Choline O-Acetyltransferase, Colchicine, Disease Models, Animal, Dose-Response Relationship, Drug, Frontal Lobe, Hippocampus, Ibotenic Acid, Male, Medicine, Ayurvedic, Phytotherapy, Memory Disorders, Plants, Medicinal, Receptors, Muscarinic, Rats, Wistar, Cholinergic Antagonists, Rats
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