The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Simple, Rapid, Quantitative, Syncytium-Forming Microassay for the Detection of Human Immunodeficiency Virus Neutralizing Antibody
Abstract
A simple, rapid, quantitative syncytium-forming microassay for the detection of human immunodeficiency virus (HIV-I) isolates is described. A virus-syncytial sensitive clone of CEM cells (CEM-SS) was identified and made adherent to flat bottom 96-well microtiter dishes. Following the addition of virus, these cells develop easily quantifiable, adherent syncytia on a background of confluent, normal CEM-SS monolayer in 4 to 6 days. One-hit kinetics for syncytia formation were obtained at various multiplicities of infection. Syncytia are associated with complete virion production and cytoplasmic localization of the p24 core protein (detected by immunofluorescence). Total infectious virus can be accurately determined in this assay; these results showed a close correlation with p24 and gp120 induction when microtiter well supernatants were passed to fresh cells and evaluated by competitive radioimmunoassay. Studies of p24 antigen induction at and beyond the end point of syncytia formation indicate that there are no detectable nonsyncytial variants in standard HIV-I stocks. Six divergent HIV-I isolates (HTLV-IIIB, -RFII, -MN, -RUTZ, -CC, and LAV-1), as well as HTLV-IIIB and LAV-1 reisolated from persistently infected chimpanzees, produce quantifiable syncytia which vary slightly in their developmental morphology. Accurate neutralization titers are readily obtained from easily constructed multiplicity curves derived from serial dilutions of test sera. Inherent within this system is a flexible method for studying various kinetics of antibody/virus interactions, as well as blocking and interference studies with any candidate antiviral compounds.
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The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus
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Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia
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Infection of HTLV-III/LAV in HTLV-I-Carrying Cells MT-2 and MT-4 and Application in a Plaque Assay
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Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicity
Sodroski, J., Goh, W. C., Rosen, C., Campbell, K., & Haseltine, W. A. (1986) · Nature · doi:10.1038/322470a0 · Cited by 798
Production of Plaques in Monolayer Tissue Cultures by Single Particles of an Animal Virus
Dulbecco, R. (1952) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.38.8.747 · Cited by 667
Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients
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HTLV-III-neutralizing antibodies in patients with AIDS and AIDS-related complex
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Sequence Homology and Morphologic Similarity of HTLV-III and Visna Virus, a Pathogenic Lentivirus
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Self-citationSuramin Protection of T Cells in Vitro Against Infectivity and Cytopathic Effect of HTLV-III
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Cited by
HIV-1 and HIV-2 exhibit divergent interactions with HLTF and UNG2 DNA repair proteins
Hrecka, K., Hao, C., Shun, M.-C., Kaur, S., Swanson, S. K., Florens, L., Washburn, M. P., & Skowronski, J. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1605023113 · Cited by 80
Analysis of the human immunodeficiency virus-1 RNA packageome
Eckwahl, M. J., Arnion, H., Kharytonchyk, S., Zang, T., Bieniasz, P. D., Telesnitsky, A., & Wolin, S. L. (2016) · RNA · doi:10.1261/rna.057299.116 · Cited by 57
EWI-2 Inhibits Cell–Cell Fusion at the HIV-1 Virological Presynapse
Whitaker, E. E., Matheson, N. J., Perlee, S., Munson, P. B., Symeonides, M., & Thali, M. (2019) · Viruses · doi:10.3390/v11121082 · Cited by 27
Metadata
- Authors
- NARA, P. L., HATCH, W. C., DUNLOP, N. M., ROBEY, W. G., ARTHUR, L. O., GONDA, M. A., & FISCHINGER, P. J.
- Venue
- AIDS Research and Human Retroviruses
- Publisher
- SAGE Publications
- Published
- 1987-01
- Volume
- 3
- Issue
- 3
- Pages
- 283-302
- ISSN
- 0889-2229 (ISSN-L)
- 1931-8405
- OpenAlex
- W1984836566
- Cited by
- 272
- References
- 15
- FWCI
- 3.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Simple, Rapid, Quantitative, Syncytium-Forming Microassay for the Detection of Human Immunodeficiency Virus Neutralizing Antibody. https://app.cochat.ai/papers/10.1089/aid.1987.3.283
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- NARA, P. L., HATCH, W. C., DUNLOP, N. M., ROBEY, W. G., ARTHUR, L. O., GONDA, M. A., & FISCHINGER, P. J. (1987). Simple, Rapid, Quantitative, Syncytium-Forming Microassay for the Detection of Human Immunodeficiency Virus Neutralizing Antibody. AIDS Research and Human Retroviruses. https://doi.org/10.1089/aid.1987.3.283
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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