Cited by 41Regulation of Appetite and ObesityNeuropeptides and Animal PhysiologyEating Disorders and Behaviors

Role of Reactive Oxygen Species-Related Enzymes in Neuropeptide Y and Proopiomelanocortin-Mediated Appetite Control: A Study Using Atypical Protein Kinase C Knockdown

Kuo, Dong-Yih, Chen, Pei-Ni, Yang, Shun-Fa, Chu, Shu-Chen, Chen, Chin-Hsiu, Kuo, Meng-Hsien, Yu, Ching-Han, Hsieh, Yih-Shou·2011·Antioxidants & Redox Signaling

Abstract

Aims: Studies have reported that redox signaling in the hypothalamus participates in nutrient sensing. The current study aimed to determine if the activation of reactive oxygen species-related enzymes (ROS-RE) in the hypothalamus participates in regulating neuropeptide Y (NPY)-mediated eating. Moreover, possible roles of proopiomelanocortin (POMC) and atypical protein kinase C (aPKC) were also investigated. Rats were treated daily with phenylpropanolamine (PPA) for 4 days. Changes in the expression levels of ROS-RE, POMC, NPY, and aPKC were assessed and compared. Results: Results showed that ROS-RE, POMC, and aPKC increased, with a maximal response on Day 2 (anorectic effect) and with a restoration to the normal level on Day 4 (tolerant effect). By contrast, NPY expression decreased, and the expression pattern of NPY proved opposite those of ROS-RE and POMC. Central inhibition of ROS production by ICV infusion of ROS scavenger attenuated PPA anorexia, revealing a crucial role of ROS in regulating eating. Cerebral aPKC knockdown by ICV infusion of antisense aPKC modulated the expression of ROS-RE, POMC, and NPY. Conclusion: Results suggest that ROS-RE/POMC- and NPY-containing neurons function reciprocally in regulating both the anorectic and tolerant effects of PPA, while aPKC is upstream of these regulators. Innovation: These results may further the understanding of ROS-RE and aPKC in the control of PPA anorexia. Antioxid. Redox Signal. 15, 2147–2159.

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Crossref · 10.1089/ars.2010.3738

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  • Various kinases and transcription factors involved in neuropeptide regulation have been reported to be modulated by redox signaling.

    “Various kinases and transcription factors, involved in neuropeptide regulation, have been reported to be modulated by redox signaling ([33](#B33), [91](#B91)).”

    From Mitochondrial fatty acid oxidation in obesity (2013) · cites this paper as [91]

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Authors
Kuo, D.-Y., Chen, P.-N., Yang, S.-F., Chu, S.-C., Chen, C.-H., Kuo, M.-H., Yu, C.-H., & Hsieh, Y.-S.
Affiliations
  • Chung Shan Medical University Hospital (TW)
  • Central Taiwan University of Science and Technology (TW)
  • National Taichung Second Senior High School
Corresponding author
Hsieh, Yih-Shou
Venue
Antioxidants & Redox Signaling
Published
2011-10
Volume
15
Issue
8
Pages
2147-2159
ISSN
  • 1523-0864 (ISSN-L)
  • 1557-7716
OpenAlex
W2080681608
Cited by
41
References
63
Citations by year
2025: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 3 · 2012: 8
FWCI
2.38
Type
journal article
Language
en
Open access
closed
Locations
  • Antioxidants and Redox Signaling (publishedVersion)
  • PubMed (publishedVersion)
Funders
Chung Shan Medical University
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Social Sciences › Psychology › Clinical Psychology › Eating Disorders and Behaviors
Keywords
reactive oxygen species, neuropeptide Y, proopiomelanocortin, atypical protein kinase C, appetite regulation, phenylpropanolamine, anorexia, tolerance (pharmacology and immunology), hypothalamus, redox signaling, ROS scavengers, antisense knockdown, dietary habits
MeSH terms
Animals, Appetite Regulation, Male, Neuropeptide Y, Phenylpropanolamine, Pro-Opiomelanocortin, Protein Kinase C, Blotting, Western, Rats, Wistar, Reactive Oxygen Species, Reverse Transcriptase Polymerase Chain Reaction, Rats

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