The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Assessment of Recombinant Adenoviral Vectors for Hepatic Gene Therapy
Abstract
Recombinant adenoviral vectors have recently been used to transfer genes into a number of different cell types in vitro and in vivo. A recombinant adenoviral vector bearing the Escherichia coli β-galactosidase (β-gal) gene was used to quantitate the frequency of hepatocyte transduction in the mouse after direct viral infusion into the portal vein. When 1010 adenoviral particles were infused, over 95% of the hepatocytes were transduced in vivo as determined by x-gal staining. The transduction protocol is relatively safe in that there is no detectable helper virus production in transduced animals and that very few extrahepatic cells are transduced by this method. There is also no evidence of significant liver pathology unless substantially greater quantities of virus are used. However, the transduced hepatocytes do not appear to persist in vivo because the percentage of hepatocytes expressing β-gal declined over time. Four months after the procedure, 0.5–10% of the hepatocytes contain detectable β-gal activity in vivo. The change in β-gal-positive cells correlates with decreasing amounts of adenoviral DNA. Thus, current recombinant adenoviral vectors may have clinical applications in gene therapy for acute hepatic disorders. This study demonstrates that the recombinant adenovirus can be used to transfer genes into greater than 95% of mouse hepatocytes in vivo. The expression from the majority of transduced hepatocytes is slowly lost over a 3- to 4-month period. This vector system has the advantage of being a very efficient means of gene transfer into the liver; the disadvantage of the system relates to the transient nature of expression in most of the transduced hepatocytes. Further experimentation will be required to determine whether a significant number of transduced hepatocytes will remain indefintely.
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Rosenfeld, M. A., Yoshimura, K., Trapnell, B. C., Yoneyama, K., Rosenthal, E. R., Dalemans, W., Fukayama, M., Bargon, J., Stier, L. E., Stratford-Perricaudet, L., et al. (1992) · Cell · doi:10.1016/0092-8674(92)90213-v · Cited by 931
Self-citationAdenovirus-Mediated Transfer of a Recombinant α1-Antitrypsin Gene to the Lung Epithelium in Vivo
Rosenfeld, M. A., Siegfried, W., Yoshimura, K., Yoneyama, K., Fukayama, M., Stier, L. E., Pääkkö, P. K., Gilardi, P., Stratford-Perricaudet, L. D., Perricaudet, M., et al. (1991) · Science · doi:10.1126/science.2017680 · Cited by 737
Self-citationWidespread long-term gene transfer to mouse skeletal muscles and heart.
Stratford-Perricaudet, L. D., Makeh, I., Perricaudet, M., & Briand, P. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115902 · Cited by 580
Self-citationAdenovirus–mediated in vivo gene transfer and expression in normal rat liver
Jaffe, H. A., Danel, C., Longenecker, G., Metzger, M., Setoguchi, Y., Rosenfeld, M. A., Gant, T. W., Thorgeirsson, S. S., Stratford-Perricaudet, L. D., Perricaudet, M., et al. (1992) · Nature Genetics · doi:10.1038/ng0892-372 · Cited by 449
Self-citationPonder, K. P., Gupta, S., Leland, F., Darlington, G., Finegold, M., DeMayo, J., Ledley, F. D., Chowdhury, J. R., & Woo, S. L. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.4.1217 · Cited by 369
Self-citationLong-Term Improvement of Hypercholesterolemia After Ex Vivo Gene Therapy in LDLR-Deficient Rabbits
Chowdhury, J. R., Grossman, M., Gupta, S., Chowdhury, N. R., Baker, J. R., & Wilson, J. M. (1991) · Science · doi:10.1126/science.1722351 · Cited by 366
Stratford-Perricaudet, L. D., Levrero, M., Chasse, J.-F., Perricaudet, M., & Briand, P. (1990) · Human Gene Therapy · doi:10.1089/hum.1990.1.3-241 · Cited by 325
Self-citationAdenovirus as an expression vector in muscle cells in vivo.
Quantin, B., Perricaudet, L. D., Tajbakhsh, S., & Mandel, J. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.7.2581 · Cited by 296
Retroviral-mediated gene transfer into hepatocytes in vivo.
Ferry, N., Duplessis, O., Houssin, D., Danos, O., & Heard, J. M. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.19.8377 · Cited by 285
Kay, M. A., Baley, P., Rothenberg, S., Leland, F., Fleming, L., Ponder, K. P., Liu, T., Finegold, M., Darlington, G., & Pokorny, W. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.89 · Cited by 245
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Cited by
Induced pluripotent stem cells generated without viral integration
Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G., & Hochedlinger, K. (2008) · Science · doi:10.1126/science.1162494 · Cited by 1,683
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280
Engineering targeted viral vectors for gene therapy
Waehler, R., Russell, S. J., & Curiel, D. T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2141 · Cited by 687
Overturf, K., Al-Dhalimy, M., Tanguay, R., Brantly, M., Ou, C.-N., Finegold, M., & Grompe, M. (1996) · Nature Genetics · doi:10.1038/ng0396-266 · Cited by 549
Self-citationThe role of TGFβ1 in initiating hepatic stellate cell activation in vivo
Hellerbrand, C., Stefanovic, B., Giordano, F., Burchardt, E. R., & Brenner, D. A. (1999) · Journal of Hepatology · doi:10.1016/s0168-8278(99)80010-5 · Cited by 393
Metadata
- Authors
- Li, Q., Kay, M. A., Finegold, M., Stratford-Perricaudet, L. D., & Woo, S. L. C.
- Venue
- Human Gene Therapy
- Publisher
- SAGE Publications
- Published
- 1993-08
- Volume
- 4
- Issue
- 4
- Pages
- 403-409
- ISSN
- 1043-0342 (ISSN-L)
- 1557-7422
- OpenAlex
- W2094646089
- Cited by
- 305
- References
- 12
- FWCI
- 16.18
- Type
- journal article
- Language
- en
- Open access
- closed
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