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Black Soybean Extract Protects Against TMT-Induced Cognitive Defects in Mice
Open accessAbstract
To find a neuroactive compound with a potent inhibitory effect on acetylcholinesterase (AChE) and in vivo anti-amnesic activity from natural resources, we evaluated anthocyanins and nonanthocyanins from black soybean extract. Nonanthocyanins from black soybean extract were the most potent and dose-dependent AChE inhibitors. Intracellular reactive oxygen species accumulation resulting from H₂O₂ treatment was significantly decreased compared with cells treated with H₂O₂ only. Nonanthocyanins were also neuroprotective against H₂O₂ treated neurotoxicity by 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay. Finally, nonanthocyanins from black soybean in the preadministration group attenuated trimethyltin (TMT)-induced memory injury in both in vivo tests. AChE, prepared from mice brain tissues, was inhibited by nonanthocyanins from black soybean in a dose-dependent manner. Malondialdehyde generation in the brain homogenates of mice treated with nonanthocyanins from black soybean was decreased. We concluded that nonanthocyanins from black soybean had an efficacious in vitro AChE inhibitory activity, and protected against H₂O₂-induced neurotoxicity. In addition, our findings suggest that nonanthocyanins from black soybean may improve the TMT-induced learning and memory deficit because of AChE inhibition of mice brain tissue. Consequently, these results demonstrate that the nonanthocyanins from black soybean could possess a wide range of beneficial activities for neurodegenerative disorders.
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Black soybean nonanthocyanin extracts (5-20 mg/kg) enhanced learning and memory, reduced oxidative stress, and inhibited AChE activity, with 20 mg/kg showing significant AChE reduction in trimethyltin-treated mice.
“black soybean nonanthocyanin extracts (5–20 mg/kg) enhanced learning and memory, reduced oxidative stress, and inhibited AChE activity, with the 20 mg/kg dose showing significant AChE reduction in TMT-treated mice [84].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [84]
The Discussion reports dose-dependent improvement in TMT-induced cognitive impairment at 5–20 mg/kg, and the Results report reduced brain MDA, a marker of oxidative stress. The Abstract and Discussion also report reduced brain AChE activity, but the passages retrieved do not establish that the reduction at 20 mg/kg specifically was statistically significant. Page numbers were not available in our copy. (LLM, conf=0.82)
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Metadata
- Authors
- Jeong, J. H., Jo, Y. N., Kim, H. J., Jin, D. E., Kim, D.-O., & Heo, H. J.
- Affiliations
- Gyeongsang National University (KR)
- Kyung Hee University (KR)
- Corresponding author
- Heo, Ho Jin
- Venue
- Journal of Medicinal Food
- Publisher
- SAGE Publications
- Published
- 2014-01
- Volume
- 17
- Issue
- 1
- Pages
- 83-91
- ISSN
- 1096-620X (ISSN-L)
- 1557-7600
- PMC
- PMC3901319
- OpenAlex
- W1795852301
- Cited by
- 11
- References
- 34
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 3
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Medicinal Food (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- National Research Foundation of Korea (KRF-2011-0021664, BK21 program)
- U.S. Forest Service
- Korea Forest Service (2013-Free10, KRF-2011-0021664)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Pharmacology (medical) › Cholinesterase and Neurodegenerative Diseases
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- anthocyanins, acetylcholinesterase inhibition, cognitive impairment, memory impairment, oxidative stress, neuroprotection, malondialdehyde, neurodegenerative diseases, antiamnesic activity
- MeSH terms
- Acetylcholinesterase, Animals, Brain, Cholinesterase Inhibitors, Cognition, Cognition Disorders, Humans, Learning, Male, Malondialdehyde, Mice, Inbred ICR, Plant Extracts, Glycine max, Thiazoles, Reactive Oxygen Species, Mice
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