The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
RNA-Seq Technology and Its Application in Fish Transcriptomics
Open accessAbstract
High-throughput sequencing technologies, also known as next-generation sequencing (NGS) technologies, have revolutionized the way that genomic research is advancing. In addition to the static genome, these state-of-art technologies have been recently exploited to analyze the dynamic transcriptome, and the resulting technology is termed RNA sequencing (RNA-seq). RNA-seq is free from many limitations of other transcriptomic approaches, such as microarray and tag-based sequencing method. Although RNA-seq has only been available for a short time, studies using this method have completely changed our perspective of the breadth and depth of eukaryotic transcriptomes. In terms of the transcriptomics of teleost fishes, both model and non-model species have benefited from the RNA-seq approach and have undergone tremendous advances in the past several years. RNA-seq has helped not only in mapping and annotating fish transcriptome but also in our understanding of many biological processes in fish, such as development, adaptive evolution, host immune response, and stress response. In this review, we first provide an overview of each step of RNA-seq from library construction to the bioinformatic analysis of the data. We then summarize and discuss the recent biological insights obtained from the RNA-seq studies in a variety of fish species.
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Cited by
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Sulfonamide functional head on short-chain perfluorinated substance drives developmental toxicity
Rericha, Y., Cao, D., Truong, L., Simonich, M. T., Field, J. A., & Tanguay, R. L. (2022) · iScience · doi:10.1016/j.isci.2022.103789 · Cited by 64
Metadata
- Authors
- Qian, X., Ba, Y., Zhuang, Q., & Zhong, G.
- Venue
- OMICS: A Journal of Integrative Biology
- Publisher
- SAGE Publications
- Published
- 2014-02
- Volume
- 18
- Issue
- 2
- Pages
- 98-110
- ISSN
- 1536-2310 (ISSN-L)
- 1557-8100
- OpenAlex
- W2071756586
- Cited by
- 350
- References
- 16
- FWCI
- 6.32
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Qian, X., Ba, Y., Zhuang, Q., & Zhong, G. (2014). RNA-Seq Technology and Its Application in Fish Transcriptomics. OMICS: A Journal of Integrative Biology. https://doi.org/10.1089/omi.2013.0110
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