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Cited by 611Hydrogels: synthesis, properties, applicationsCollagen: Extraction and Characterization3D Printing in Biomedical Research

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

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Antoine, Elizabeth E., Vlachos, Pavlos P., Rylander, Marissa Nichole·2014·Tissue Engineering Part B: Reviews
DOI 10.1089/ten.teb.2014.0086PMC PMC4241868
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Abstract

Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagen-hydrogel-based characterization studies and tissue engineering experiments.

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Authors
Antoine, E. E., Vlachos, P. P., & Rylander, M. N.
Affiliations
  • Virginia Tech (US)
  • Purdue University West Lafayette (US)
  • Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US)
Corresponding author
Antoine, Elizabeth E.
ORCID
Venue
Tissue Engineering Part B: Reviews
Publisher
SAGE Publications
Published
2014-12
Volume
20
Issue
6
Pages
683-696
ISSN
  • 1937-3368 (ISSN-L)
  • 1937-3376
DOI
10.1089/ten.teb.2014.0086
PMC
PMC4241868
OpenAlex
W2325212255
Cited by
611
References
7
Citations by year
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FWCI
1.09
Type
journal article
Language
en
Open access
Licence
www.liebertpub.com/nv/resources-tools/text-and-data-mining-policy/121/ (tdm)
Locations
  • Tissue Engineering Part B Reviews (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (0955072, CAREER)
  • National Cancer Institute (IR21CA158454-01A1)
  • Division of Chemical, Bioengineering, Environmental, and Transport Systems
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
collagen I hydrogel, hydrogel characterization, tissue engineering scaffolds, fabrication parameters, hydrogel mechanics, hydrogel structure, mass transfer, 3D cell culture, tissue mimics, tumor models
MeSH terms
Animals, Biological Transport, Humans, Hydrogels, Tissue Engineering, Collagen Type I, Mechanical Phenomena, Cellular Microenvironment

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