Three-dimensional Culture and Clinical Drug Responses of a Highly Metastatic Human Ovarian Cancer HO-8910PM Cells in Nanofibrous Microenvironments of Three Hydrogel Biomaterials
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Abstract Background Ovarian cancer is a highly aggressive malignant disease in gynecologic cancer. It is an urgent task to develop three-dimensional (3D) cell models in vitro and dissect the cell progression-related drug resistance mechanisms in vivo. In the present study, RADA16-I peptide has the reticulated nanofiber scaffold networks in hydrogel, which is utilized to develop robust 3D cell culture of a high metastatic human ovarian cancer HO-8910PM cell line accompanied with the counterparts of Matrigel and collagen I. Results Consequently, HO-8910PM cells were successfully cultivated in three types of hydrogel biomaterials, such as RADA16-I hydrogel, Matrigel, and collagen I, according to 3D cell culture protocols. Designer RADA16-I peptide had well-defined nanofiber networks architecture in hydrogel, which provided nanofiber cell microenvironments analogous to Matrigel and collagen I. 3D-cultured HO-8910PM cells in RADA16-I hydrogel, Matrigel, and collagen I showed viable cell proliferation, proper cell growth, and diverse cell shapes in morphology at the desired time points. For a long 3D cell culture period, HO-8910PM cells showed distinct cell aggregate growth patterns in RADA16-I hydrogel, Matrigel, and collagen I, such as cell aggregates, cell colonies, cell clusters, cell strips, and multicellular tumor spheroids (MCTS). The cell distribution and alignment were described vigorously. Moreover, the molecular expression of integrin β1, E-cadherin and N-cadherin were quantitatively analyzed in 3D-cultured MCTS of HO-8910PM cells by immunohistochemistry and western blotting assays. The chemosensitivity assay for clinical drug responses in 3D context indicated that HO-8910PM cells in three types of hydrogels showed significantly higher chemoresistance to cisplatin and paclitaxel compared to 2D flat cell culture, including IC 50 values and inhibition rates. Conclusion Based on these results, RADA16-I hydrogel is a highly competent, high-profile, and proactive nanofiber scaffold to maintain viable cell proliferation and high cell vitality in 3D cell models, which may be particularly utilized to develop useful clinical drug screening platform in vitro.
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A disadvantage of some peptide-based hydrogels is their low mechanical strength, where hydrogels with encapsulated cells are able to be disrupted easily by mechanical forces such as pipetting.
“A disadvantage of some peptide-based hydrogels is their low mechanical strength, where hydrogels with encapsulated cells are able to be disrupted easily by mechanical forces such as pipetting (Song et al., 2020).”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Song et al., 2020
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Yee, C., Dickson, K.-A., Muntasir, M. N., Ma, Y., & Marsh, D. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.836984 · Cited by 66 · see citing works
- Authors
- Song, H., Cai, G.-H., Liang, J., Ao, D.-S., Wang, H., & Yang, Z.-H.
- Affiliations
- Zunyi Medical University (CN)
- Venue
- Journal of Nanobiotechnology
- Published
- 2020-06-11
- Volume
- 18
- Issue
- 1
- Pages
- 90-90
- ISSN
- 1477-3155 (ISSN-L)
- PMC
- PMC7291456
- OpenAlex
- W3034753503
- Cited by
- 52
- References
- 78
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 8 · 2023: 5 · 2022: 14 · 2021: 12 · 2020: 2
- FWCI
- 2.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Nanobiotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81160290, 81660340)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- ovarian cancer, HO-8910PM cells, nanofibrous hydrogels, 3D cell culture, tumor spheroids, chemoresistance, cisplatin, paclitaxel, Matrigel, type I collagen, beta1 integrin, E-cadherin, N-cadherin, cell alignment, drug screening
- MeSH terms
- Antineoplastic Agents, Biocompatible Materials, Female, Humans, Ovarian Neoplasms, Cell Culture Techniques, Hydrogels, Cell Line, Tumor, Cell Proliferation, Nanofibers, Tumor Microenvironment
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