Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold
Open accessAbstract
The purpose of this study was to evaluate the effects of alginate composition on the neurotrophic factor release, viability, and proliferation of encapsulated neural stem cells (NSCs), as well as on the mechanical stability of the scaffold itself. Four compositions were tested: a high guluronic acid (68%) and a high mannuronic acid (54%) content alginate, with or without a poly-L-lysine (PLL) coating layer. Enzyme-linked immunosorbent assay was used to quantify the release of brain-derived neurotrophic factor, glial-derived neurotrophic factor, and nerve growth factor from the encapsulated cells. All three factors were detected from encapsulated cells only when a high L-guluronic acid alginate without PLL was used. Additionally, capsules with this composition remained intact more frequently when exposed to solutions of low osmolarity, potentially indicating superior mechanical stability. Alginate beads with a PLL-coated, high D-mannuronic acid composition were the most prone to breakage in the osmotic pressure test, and were too fragile for histology and proliferation assays after 1 week in vitro. NSCs survived and proliferated in the three remaining alginate compositions similarly over the 21-day study course irrespective of scaffold condition. NSC-seeded alginate beads with a high L-guluronic acid, non-PLL-coated composition may be useful in the repair of injured nervous tissue, where the mechanism is the secretion of neuroprotective factors. We verify the neuroprotective effects of medium conditioned by NSC-seeded alginate beads on the serum withdrawal-mediated death of PC-12 cells here.
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Hydrogel scaffolding with neural stem cells produced an initial benefit to surrounding neuronal density within the first week of transplantation.
“In the case of hydrogel scaffolding, an initial benefit to surrounding neuronal density was noted within the first week of transplantation, which may be related to release of neurotrophic factors by the engrafted cells.89”
From Regenerative Electrode Interfaces for Neural Prostheses. (2016) · cites this paper as [89]
The Discussion reports that alginate-encapsulated neural stem cells secrete neurotrophic factors, and Figure 4 reports a protective effect of their conditioned medium on cultured PC-12 cells. The paper does not report transplantation or a change in surrounding neuronal density within the first week; it describes testing the scaffolded cells’ ability to repair nervous tissue as future work. The proposed mechanism is supported, but the claimed transplant outcome is not. (LLM, conf=0.85)
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Regenerative Electrode Interfaces for Neural Prostheses.
Thompson, C. H., Zoratti, M. J., Langhals, N. B., & Purcell, E. K. (2016) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2015.0279 · Cited by 67 · see citing works
Self-citation
- Authors
- Purcell, E. K., Singh, A., & Kipke, D. R.
- Affiliations
- University of Michigan (US)
- Venue
- Tissue Engineering Part C: Methods
- Publisher
- SAGE Publications
- Published
- 2009-12
- Volume
- 15
- Issue
- 4
- Pages
- 541-550
- ISSN
- 1937-3384 (ISSN-L)
- 1937-3392
- PMC
- PMC2819710
- OpenAlex
- W2069824861
- Cited by
- 95
- References
- 56
- Citations by year
- 2025: 5 · 2024: 1 · 2023: 7 · 2022: 4 · 2021: 7 · 2020: 4 · 2019: 7 · 2018: 6 · 2017: 9 · 2016: 3 · 2015: 9 · 2014: 10 · 2013: 5 · 2012: 9
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part C Methods (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Deep Blue (University of Michigan) (submittedVersion)
- Funders
- National Science Foundation
- University of Michigan
- National Institute of Biomedical Imaging and Bioengineering (P41EB002030)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- alginate, neural stem cells, guluronic acid, mannuronic acid, poly-L-lysine coating, brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor, nerve growth factor, encapsulated cells, osmotic pressure, cell viability and proliferation, neuroprotection, serum deprivation, PC12 cells
- MeSH terms
- Alginates, Animals, Biomechanical Phenomena, Cell Survival, Enzyme-Linked Immunosorbent Assay, Glial Fibrillary Acidic Protein, Hexuronic Acids, Immunohistochemistry, Intermediate Filament Proteins, Microspheres, Nerve Growth Factors, Nerve Tissue Proteins, Neurons, Stem Cells, PC12 Cells, Culture Media, Conditioned, Glucuronic Acid, Cell Proliferation, Mice, Rats, Tissue Scaffolds, Nestin
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