The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Endocytosis of Epithelial Apical Junctional Proteins by a Clathrin-mediated Pathway into a Unique Storage Compartment
Abstract
The adherens junction (AJ) and tight junction (TJ) are key regulators of epithelial polarity and barrier function. Loss of epithelial phenotype is accompanied by endocytosis of AJs and TJs via unknown mechanisms. Using a model of calcium depletion, we defined the pathway of internalization of AJ and TJ proteins (E-cadherin, p120 and β-catenins, occludin, JAM-1, claudins 1 and 4, and ZO-1) in T84 epithelial cells. Proteinase protection assay and immunocytochemistry revealed orchestrated internalization of AJs and TJs into a subapical cytoplasmic compartment. Disruption of caveolae/lipid rafts did not prevent endocytosis, nor did caveolin-1 colocalize with internalized junctional proteins. Furthermore, AJ and TJ proteins did not colocalize with the macropinocytosis marker dextran. Inhibitors of clathrin-mediated endocytosis blocked internalization of AJs and TJs, and junctional proteins colocalized with clathrin and α-adaptin. AJ and TJ proteins were observed to enter early endosomes followed by movement to organelles that stained with syntaxin-4 but not with markers of late and recycling endosomes, lysosomes, or Golgi. These results indicate that endocytosis of junctional proteins is a clathrin-mediated process leading into a unique storage compartment. Such mechanisms may mediate the disruption of intercellular contacts during normal tissue remodeling and in pathology.
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Araki, N., Johnson, M. T., & Swanson, J. A. (1996) · The Journal of cell biology · doi:10.1083/jcb.135.5.1249 · Cited by 949 · cited 1×
“, 2002), and the other involved inhibition of phosphoinositide (PI) 3-kinase (Araki et al., 1996; Amyere et al., 2002).”
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Cited by
Luissint, A.-C., Parkos, C. A., & Nusrat, A. (2016) · Gastroenterology · doi:10.1053/j.gastro.2016.07.008 · Cited by 555
Self-citationFasano, A., & Shea-Donohue, T. (2005) · Nature Clinical Practice Gastroenterology & Hepatology · doi:10.1038/ncpgasthep0259 · Cited by 484
Marchiando, A. M., Shen, L., Graham, W. V., Weber, C. R., Schwarz, B. T., Austin, J. R., Raleigh, D. R., Guan, Y., Watson, A. J. M., Montrose, M. H., et al. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200902153 · Cited by 477
Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure
Shen, L., Black, E. D., Witkowski, E. D., Lencer, W. I., Guerriero, V., Schneeberger, E. E., & Turner, J. R. (2006) · Journal of Cell Science · doi:10.1242/jcs.02915 · Cited by 467
Intestinal epithelial claudins: expression and regulation in homeostasis and inflammation
Garcia‐Hernandez, V., Quiros, M., & Nusrat, A. (2017) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.13360 · Cited by 445
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Metadata
- Authors
- Ivanov, A. I., Nusrat, A., & Parkos, C. A.
- Venue
- Molecular Biology of the Cell
- Published
- 2004-01
- Volume
- 15
- Issue
- 1
- Pages
- 176-188
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2148700019
- Cited by
- 383
- References
- 1
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ivanov, A. I., Nusrat, A., & Parkos, C. A. (2004). Endocytosis of Epithelial Apical Junctional Proteins by a Clathrin-mediated Pathway into a Unique Storage Compartment. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e03-05-0319
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