The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects
Open accessAbstract
Prior work in our laboratory established a connection between the PALS1/PATJ/CRB3 and Par6/Par3/aPKC protein complexes at the tight junction of mammalian epithelial cells. Utilizing a stable small interfering RNA expression system, we have markedly reduced expression of the tight junction-associated protein PALS1 in MDCKII cells. The loss of PALS1 resulted in a corresponding loss of expression of PATJ, a known binding partner of PALS1, but had no effect on the expression of CRB3. However, the absence of PALS1 and PATJ expression did result in the decreased association of CRB3 with members of the Par6/Par3/aPKC protein complex. The consequences of the loss of PALS1 and PATJ were exhibited by a delay in the polarization of MDCKII monolayers after calcium switch, a decrease in the transepithelial electrical resistance, and by the inability of these cells to form lumenal cysts when grown in a collagen gel matrix. These defects in polarity determination may be the result of the lack of recruitment of aPKC to the tight junction in PALS1-deficient cells, as observed by confocal microscopy, and subsequent alterations in downstream signaling events.
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Cites
The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
Joberty, G., Petersen, C., Gao, L., & Macara, I. G. (2000) · Nature Cell Biology · doi:10.1038/35019573 · Cited by 928 · cited 1×
“Many studies support the relationship between the Par6/Par3/aPKC complex and the formation of tight junctions (Izumi et al., 1998; Joberty et al., 2000; Ohno, 2001; Yamanaka et al., 2001; D’Atri and Citi, 2002; Gao et al., 2002; Hirose et al., 2002).”
Building epithelial architecture: insights from three-dimensional culture models
O'Brien, L. E., Zegers, M. M. P., & Mostov, K. E. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm859 · Cited by 596
Integrated activity of PDZ protein complexes regulates epithelial polarity
Bilder, D., Schober, M., & Perrimon, N. (2003) · Nature Cell Biology · doi:10.1038/ncb897 · Cited by 435
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Yamanaka, T., Horikoshi, Y., Suzuki, A., Sugiyama, Y., Kitamura, K., Maniwa, R., Nagai, Y., Yamashita, A., Hirose, T., Ishikawa, H., et al. (2001) · Genes to Cells · doi:10.1046/j.1365-2443.2001.00453.x · Cited by 306
Suzuki, A., Ishiyama, C., Hashiba, K., Shimizu, M., Ebnet, K., & Ohno, S. (2002) · Journal of Cell Science · doi:10.1242/jcs.00032 · Cited by 251
Cited by
Modelling glandular epithelial cancers in three-dimensional cultures
Debnath, J., & Brugge, J. S. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1695 · Cited by 1,015
Tight Junctions and Cell Polarity
Shin, K., Fogg, V. C., & Margolis, B. (2006) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.22.010305.104219 · Cited by 745
Self-citationAssémat, E., Bazellières, E., Pallesi-Pocachard, E., Le Bivic, A., & Massey-Harroche, D. (2008) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2007.08.029 · Cited by 470
The Mammalian Crumbs Complex Defines a Distinct Polarity Domain Apical of Epithelial Tight Junctions
Tan, B., Yatim, S. M. J. M., Peng, S., Gunaratne, J., Hunziker, W., & Ludwig, A. (2020) · Current Biology · doi:10.1016/j.cub.2020.05.032 · Cited by 98
van Rossum, A. G. S. H., Aartsen, W. M., Meuleman, J., Klooster, J., Malysheva, A., Versteeg, I., Arsanto, J.-P., Le Bivic, A., & Wijnholds, J. (2006) · Human Molecular Genetics · doi:10.1093/hmg/ddl194 · Cited by 104
Metadata
- Authors
- Straight, S. W., Shin, K., Fogg, V. C., Fan, S., Liu, C.-J., Roh, M., & Margolis, B.
- Venue
- Molecular Biology of the Cell
- Published
- 2004-04
- Volume
- 15
- Issue
- 4
- Pages
- 1981-1990
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2099626566
- Cited by
- 191
- References
- 6
- FWCI
- 10.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Straight, S. W., Shin, K., Fogg, V. C., Fan, S., Liu, C.-J., Roh, M., & Margolis, B. (2004). Loss of PALS1 Expression Leads to Tight Junction and Polarity Defects. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e03-08-0620
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