Building epithelial architecture: insights from three-dimensional culture models
Abstract
How do individual cells organize into multicellular tissues? Here, we propose that the morphogenetic behaviour of epithelial cells is guided by two distinct elements: an intrinsic differentiation programme that drives formation of a lumen-enclosing monolayer, and a growth factor-induced, transient de-differentiation that allows this monolayer to be remodelled.
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3D epithelial organoid culture in Matrigel recapitulates numerous features of glandular tissues, including fully differentiated acini that maintain apico-basal polarity by enclosing a central lumen.
“3D epithelial organoids recapitulate numerous features of glandular tissues including the development of fully differentiated acini that maintain apico-basal polarity with hollow lumen.”
From Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens (2018) · cites this paper as [5]
Cites
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Three-dimensional cell culture: the missing link in drug discovery
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Micropatterning as a tool to decipher cell morphogenesis and functions
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ZDHHC7-mediated S-palmitoylation of Scribble regulates cell polarity
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“For example, epithelial organoid culture in Matrigel recapitulates numerous features of glandular tissues including the development of fully differentiated acini that maintain apico-basal polarity by enclosing a central lumen (5).”
BackgroundSuárez-Causado, A., Caballero-Díaz, D., Bertrán, E., Roncero, C., Addante, A., García-Álvaro, M., Fernández, M., Herrera, B., Porras, A., Fabregat, I., et al. (2015) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2015.05.017 · Cited by 49 · see citing works
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Shao, C., Liu, Y., Chi, J., Ye, F., & Zhao, Y. (2021) · Engineering · doi:10.1016/j.eng.2020.06.031 · Cited by 28 · see citing works
c-Src-Mediated Epithelial Cell Migration and Invasion Regulated by PDZ Binding Site
Baumgartner, M., Radziwill, G., Lorger, M., Weiss, A., & Moelling, K. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.01024-07 · Cited by 28 · see citing works
Membrane recruitment of the polarity protein Scribble by the cell adhesion receptor TMIGD1
Thüring, E.-M., Hartmann, C., Maddumage, J. C., Javorsky, A., Michels, B. E., Gerke, V., Banks, L., Humbert, P. O., Kvansakul, M., & Ebnet, K. (2023) · Communications Biology · doi:10.1038/s42003-023-05088-3 · Cited by 7 · see citing works
- Authors
- O'Brien, L. E., Zegers, M. M. P., & Mostov, K. E.
- Affiliations
- University of California, San Francisco (US)
- Corresponding author
- Mostov, Keith E.
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2002-07
- Volume
- 3
- Issue
- 7
- Pages
- 531-537
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- OpenAlex
- W2062835218
- Cited by
- 596
- References
- 1
- Citations by year
- 2026: 9 · 2025: 8 · 2024: 12 · 2023: 15 · 2022: 15 · 2021: 15 · 2020: 11 · 2019: 26 · 2018: 27 · 2017: 16 · 2016: 30 · 2015: 30 · 2014: 26 · 2013: 29 · 2012: 38
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- epithelial morphogenesis, epithelial architecture, three-dimensional culture model, lumen formation, epithelial differentiation, dedifferentiation, growth factor signaling, tissue organization, cell-cell adhesion
- MeSH terms
- Animals, Cell Communication, Cell Differentiation, Cell Division, Epithelial Cells, Humans, Morphogenesis, Hepatocyte Growth Factor, Cell Culture Techniques
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