The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
The Entire Nup107-160 Complex, Including Three New Members, Is Targeted as One Entity to Kinetochores in Mitosis
Open accessAbstract
In eukaryotes, bidirectional transport of macromolecules between the cytoplasm and the nucleus occurs through elaborate supramolecular structures embedded in the nuclear envelope, the nuclear pore complexes (NPCs). NPCs are composed of multiple copies of ∼30 different proteins termed nucleoporins, of which several can be biochemically isolated as subcomplexes. One such building block of the NPC, termed the Nup107-160 complex in vertebrates, was so far demonstrated to be composed of six different nucleoporins. Here, we identify three WD (Trp-Asp)-repeat nucleoporins as new members of this complex, two of which, Nup37 and Nup43, are specific to higher eukaryotes. The third new member Seh1 is more loosely associated with the Nup107-160 complex biochemically, but its depletion by RNA interference leads to phenotypes similar to knock down of other constituents of this complex. By combining green fluorescent protein-tagged nucleoporins and specific antibodies, we show that all the constituents of this complex, including Nup37, Nup43, Seh1, and Sec13, are targeted to kinetochores from prophase to anaphase of mitosis. Together, our results indicate that the entire Nup107-160 complex, which comprises nearly one-third of the so-far identified nucleoporins, specifically localizes to kinetochores in mitosis.
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Cited by
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Components of Coated Vesicles and Nuclear Pore Complexes Share a Common Molecular Architecture
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Integrated Structural Analysis of the Human Nuclear Pore Complex Scaffold
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Metadata
- Authors
- Loïodice, I., Alves, A., Rabut, G., van Overbeek, M., Ellenberg, J., Sibarita, J.-B., & Doye, V.
- Venue
- Molecular Biology of the Cell
- Published
- 2004-07
- Volume
- 15
- Issue
- 7
- Pages
- 3333-3344
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2164516780
- Cited by
- 266
- References
- 45
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Loïodice, I., Alves, A., Rabut, G., van Overbeek, M., Ellenberg, J., Sibarita, J.-B., & Doye, V. (2004). The Entire Nup107-160 Complex, Including Three New Members, Is Targeted as One Entity to Kinetochores in Mitosis. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e03-12-0878
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