The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells
Open accessAbstract
Actin-depolymerizing factor (ADF)/cofilins are small actin-binding proteins found in all eukaryotes. In vitro, ADF/cofilins promote actin dynamics by depolymerizing and severing actin filaments. However, whether ADF/cofilins contribute to actin dynamics in cells by disassembling “old” actin filaments or by promoting actin filament assembly through their severing activity is a matter of controversy. Analysis of mammalian ADF/cofilins is further complicated by the presence of multiple isoforms, which may contribute to actin dynamics by different mechanisms. We show that two isoforms, ADF and cofilin-1, are expressed in mouse NIH 3T3, B16F1, and Neuro 2A cells. Depleting cofilin-1 and/or ADF by siRNA leads to an accumulation of F-actin and to an increase in cell size. Cofilin-1 and ADF seem to play overlapping roles in cells, because the knockdown phenotype of either protein could be rescued by overexpression of the other one. Cofilin-1 and ADF knockdown cells also had defects in cell motility and cytokinesis, and these defects were most pronounced when both ADF and cofilin-1 were depleted. Fluorescence recovery after photobleaching analysis and studies with an actin monomer-sequestering drug, latrunculin-A, demonstrated that these phenotypes arose from diminished actin filament depolymerization rates. These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility.
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1 retracted
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RetractedDuplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
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Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase
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Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
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Cited by
Stress fibers are generated by two distinct actin assembly mechanisms in motile cells
Hotulainen, P., & Lappalainen, P. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200511093 · Cited by 934
Self-citationActin in dendritic spines: connecting dynamics to function
Hotulainen, P., & Hoogenraad, C. C. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201003008 · Cited by 815
Self-citationADF/Cofilin: a functional node in cell biology
Bernstein, B. W., & Bamburg, J. R. (2010) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.01.001 · Cited by 704
Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation
Mizuno, K. (2013) · Cellular Signalling · doi:10.1016/j.cellsig.2012.11.001 · Cited by 396
Hotulainen, P., Llano, O., Smirnov, S., Tanhuanpää, K., Faix, J., Rivera, C., & Lappalainen, P. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200809046 · Cited by 360
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Metadata
- Authors
- Hotulainen, P., Paunola, E., Vartiainen, M. K., & Lappalainen, P.
- Venue
- Molecular Biology of the Cell
- Published
- 2005-02
- Volume
- 16
- Issue
- 2
- Pages
- 649-664
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W1994844322
- Cited by
- 383
- References
- 43
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- green
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- Hotulainen, P., Paunola, E., Vartiainen, M. K., & Lappalainen, P. (2005). Actin-depolymerizing Factor and Cofilin-1 Play Overlapping Roles in Promoting Rapid F-Actin Depolymerization in Mammalian Nonmuscle Cells. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e04-07-0555
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