The DOI matches its Crossref record; 57 of the 58 references it cites were found in a registry or an index.
Apical Sterol-rich Membranes Are Essential for Localizing Cell End Markers That Determine Growth Directionality in the Filamentous Fungus Aspergillus nidulans
Open accessAbstract
In filamentous fungi, hyphal extension depends on the continuous delivery of vesicles to the growing tip. Here, we describe the identification of two cell end marker proteins, TeaA and TeaR, in Aspergillus nidulans, corresponding to Tea1 and Mod5 in Schizosaccharomyces pombe. Deletion of teaA or teaR caused zig-zag-growing and meandering hyphae, respectively. The Kelch-repeat protein TeaA, the putatively prenylated TeaR protein, and the formin SepA were highly concentrated in the Spitzenkörper, a vesicle transit station at the tip, and localized along the tip membrane. TeaA localization at tips depended on microtubules, and TeaA was required for microtuble convergence in the hyphal apex. The CENP-E family kinesin KipA was necessary for proper localization of TeaA and TeaR, but not for their transportation. TeaA and TeaR localization were interdependent. TeaA interacted in vivo with TeaR, and TeaA colocalized with SepA. Sterol-rich membrane domains localized at the tip in teaA and teaR mutants like in wild type, and filipin treatment caused mislocalization of both proteins. This suggests that sterol-rich membrane domains determine cell end factor destinations and thereby polarized growth.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Interestingly, Takeshita et al. (2008) recently observed that localization of key deposition proteins involved in polarized growth at the hyphal tip requires apical sterol-rich membranes [52].”
From A Sterol-Regulatory Element Binding Protein Is Required for Cell Polarity, Hypoxia Adaptation, Azole Drug Resistance, and Virulence in Aspergillus fumigatus (2008) · cites this paper as [52]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Transformation of Aspergillus nidulans by using a trpC plasmid.
Yelton, M., Hamer, J. E., & Timberlake, W. E. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1470 · Cited by 782
Adaptation of core mechanisms to generate cell polarity
Nelson, W. J. (2003) · Nature · doi:10.1038/nature01602 · Cited by 716
The Ashbya gossypii Genome as a Tool for Mapping the Ancient Saccharomyces cerevisiae Genome
Dietrich, F. S., Voegeli, S., Brachat, S., Lerch, A., Gates, K., Steiner, S., Mohr, C., Pöhlmann, R., Luedi, P. P., Choi, S., et al. (2004) · Science · doi:10.1126/science.1095781 · Cited by 633
Lipid rafts and membrane dynamics
Rajendran, L., & Simons, K. (2005) · Journal of Cell Science · doi:10.1242/jcs.01681 · Cited by 590
Rothberg, K. G., Ying, Y. S., Kamen, B. A., & Anderson, R. G. W. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.111.6.2931 · Cited by 532
Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkorper.
Grove, S. N., & Bracker, C. E. (1970) · Journal of Bacteriology · doi:10.1128/jb.104.2.989-1009.1970 · Cited by 432
Fischer-Parton, S., Parton, R. M., Hickey, P. C., Dijksterhuis, J., Atkinson, H. A., & Read, N. D. (2000) · Journal of Microscopy · doi:10.1046/j.1365-2818.2000.00708.x · Cited by 422
Mata, J., & Nurse, P. (1997) · Cell · doi:10.1016/s0092-8674(00)80279-2 · Cited by 353
Hill, T. W., & Käfer, E. (2001) · Fungal Genetics Reports · doi:10.4148/1941-4765.1173 · Cited by 349
Howard, R. J. (1981) · Journal of Cell Science · doi:10.1242/jcs.48.1.89 · Cited by 306
Polarisome Meets Spitzenkörper: Microscopy, Genetics, and Genomics Converge
Harris, S. D., Read, N. D., Roberson, R. W., Shaw, B. D., Seiler, S., Plamann, M., & Momany, M. (2005) · Eukaryotic Cell · doi:10.1128/ec.4.2.225-229.2005 · Cited by 280
Sheu, Y.-J., Santos, B., Fortin, N. Y., Costigan, C., & Snyder, M. P. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.7.4053 · Cited by 255
Cell surface polarization during yeast mating
Bagnat, M., & Simons, K. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172517799 · Cited by 235
Lipid Raft Polarization Contributes to Hyphal Growth in Candida albicans
Martin, S. W., & Konopka, J. B. (2004) · Eukaryotic Cell · doi:10.1128/ec.3.3.675-684.2004 · Cited by 235
Genetic Analysis of the Bipolar Pattern of Bud Site Selection in the Yeast Saccharomyces cerevisiae
Zahner, J. E., Harkins, H. A., & Pringle, J. R. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1857 · Cited by 235
Tea4p Links Microtubule Plus Ends with the Formin For3p in the Establishment of Cell Polarity
Martin, S. G., McDonald, W. H., Yates, J. R., & Chang, F. (2005) · Developmental Cell · doi:10.1016/j.devcel.2005.02.008 · Cited by 213
Polarity in filamentous fungi: moving beyond the yeast paradigm
Harris, S. D., & Momany, M. (2003) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2003.11.007 · Cited by 210
Tracing the endocytic pathway of Aspergillus nidulans with FM4-64
Peñalva, M. A. (2005) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2005.09.004 · Cited by 209
What determines growth direction in fungal hyphae?
Riquelme, M., Reynaga-Peña, C. G., Gierz, G., & Bartnicki-Garcı́a, S. (1998) · Fungal Genetics and Biology · doi:10.1006/fgbi.1998.1074 · Cited by 207
Crampin, H., Finley, K. R., Gerami‐Nejad, M., Court, H., Gale, C. A., Berman, J. G., & Sudbery, P. E. (2005) · Journal of Cell Science · doi:10.1242/jcs.02414 · Cited by 195
Der Spitzenkörper von Polystictus versicolor (L.)
Girbardt, M. (1957) · Planta · doi:10.1007/bf01912343 · Cited by 183
Establishment of Cell Polarity in YeastPreprint
Pringle, J. R., Bi, E., Harkins, H. A., Zahner, J. E., VIRGILIO, C. D., Chant, J., Corrado, K., & Fares, H. (1995) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1995.060.01.079 · Cited by 176
Zhang, J., Li, S., Fischer, R., & Xiang, X. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-08-0516 · Cited by 172
Sterol-Rich Plasma Membrane Domains in Fungi
Álvarez, F. J., Douglas, L. M., & Konopka, J. B. (2007) · Eukaryotic Cell · doi:10.1128/ec.00008-07 · Cited by 168
Functional Characterization and Localization of the Aspergillus nidulans Formin SEPA
Sharpless, K. E., & Harris, S. D. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.01-07-0356 · Cited by 164
Sterol-rich plasma membrane domains in the fission yeastSchizosaccharomyces pombe
Wachtler, V., Rajagopalan, S., & Balasubramanian, M. K. (2003) · Journal of Cell Science · doi:10.1242/jcs.00299 · Cited by 164
Snell, V., & Nurse, P. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06481.x · Cited by 157
Konzack, S., Rischitor, P. E., Enke, C., & Fischer, R. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-02-0083 · Cited by 152
Tea2p Kinesin Is Involved in Spatial Microtubule Organization by Transporting Tip1p on Microtubules
Busch, K. E., Hayles, J., Nurse, P., & Brunner, D. (2004) · Developmental Cell · doi:10.1016/j.devcel.2004.05.008 · Cited by 137
Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips
Snaith, H. A., & Sawin, K. E. (2003) · Nature · doi:10.1038/nature01672 · Cited by 135
Tea2p Is a Kinesin-like Protein Required to Generate Polarized Growth in Fission Yeast
Browning, H., Hayles, J., Mata, J., Aveline-Wolf, L. D., Nurse, P., & McIntosh, J. R. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.1.15 · Cited by 134
Regulation of a formin complex by the microtubule plus end protein tea1p
Feierbach, B., Verde, F., & Chang, F. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200403090 · Cited by 130
Chang, F., & Peter, M. (2003) · Nature Cell Biology · doi:10.1038/ncb0403-294 · Cited by 123
Dynamics of the Formin For3p in Actin Cable Assembly
Martin, S. G., & Chang, F. (2006) · Current Biology · doi:10.1016/j.cub.2006.04.040 · Cited by 121
Targeted movement of cell end factors in fission yeast
Browning, H., Hackney, D. D., & Nurse, P. (2003) · Nature Cell Biology · doi:10.1038/ncb1034 · Cited by 120
Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Hyphal Growth of the FungusUstilago maydis
Schuchardt, I., Daniela, A., Thines, E., Schuberth, C. E., & Steinberg, G. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-04-0272 · Cited by 116
A GDP/GTP Exchange Factor Involved in Linking a Spatial Landmark to Cell Polarity
Kang, P. J., Sanson, A. M., Lee, B., & Park, H.-O. (2001) · Science · doi:10.1126/science.1060360 · Cited by 114
Vienken, K., & Fischer, R. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05257.x · Cited by 111
Toews, M. W., Warmbold, J., Konzack, S., Rischitor, P. E., Veith, D., Vienken, K., Vinuesa, C., Wei, H., & Fischer, R. (2004) · Current Genetics · doi:10.1007/s00294-004-0495-7 · Cited by 110
Knechtle, P., Dietrich, F. S., & Philippsen, P. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-03-0167 · Cited by 102
Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast
Snaith, H. A., Samejima, I., & Sawin, K. E. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600838 · Cited by 99
Motegi, F., Arai, R., & Mabuchi, I. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.5.1367 · Cited by 96
Philips, J. A., & Herskowitz, I. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.2.375 · Cited by 92
MesA, a Novel Fungal Protein Required for the Stabilization of Polarity Axes inAspergillus nidulans
Pearson, C. L., Xu, K., Sharpless, K. E., & Harris, S. D. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-11-0803 · Cited by 90
Nichols, C. B., Fraser, J. A., & Heitman, J. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-05-0370 · Cited by 86
Septation and cytokinesis in fungi
Walther, A., & Wendland, J. (2003) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2003.08.005 · Cited by 83
Yeast lipid rafts? – An emerging view
Wachtler, V., & Balasubramanian, M. K. (2005) · Trends in Cell Biology · doi:10.1016/j.tcb.2005.11.008 · Cited by 79
Virag, A., & Harris, S. D. (2006) · Eukaryotic Cell · doi:10.1128/ec.00036-06 · Cited by 67
CLIP-170 Homologue and NUDE Play Overlapping Roles in NUDF Localization inAspergillus nidulans
Efimov, V. P., Zhang, J., & Xiang, X. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-11-1084 · Cited by 58
Karos, M., & Fischer, R. (1999) · Molecular and General Genetics MGG · doi:10.1007/s004380050924 · Cited by 51
Philippsen, P., Kaufmann, A. M., & Schmitz, H. (2005) · Current Opinion in Microbiology · doi:10.1016/j.mib.2005.06.021 · Cited by 47
Optical tweezer micromanipulation of filamentous fungi
Wright, G. D., Arlt, J., Poon, W. C. K., & Read, N. D. (2006) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2006.07.002 · Cited by 44
Analysis of the landmark protein Bud3 of Ashbya gossypii reveals a novel role in septum construction
Wendland, J. (2003) · EMBO Reports · doi:10.1038/sj.embor.embor727 · Cited by 38
Enke, C., Zekert, N., Veith, D., Schaaf, C., Konzack, S., & Fischer, R. (2007) · Eukaryotic Cell · doi:10.1128/ec.00266-06 · Cited by 23
Cell Polarity: A New Mod(e) of Anchoring
Martin, S. G., & Chang, F. (2003) · Current Biology · doi:10.1016/j.cub.2003.08.046 · Cited by 19
Septation and Cytokinesis in Fungi
Wendland, J., & Walther, A. (2006) · Springer eBooks · doi:10.1007/3-540-28135-5_6 · Cited by 10
Research works printed but not linked: 2
- Hill T. W., 2001, Fungal Genet. Newsl, 48, 20
- Riquelme M., 1998, Biol, 24, 101
Cited by
Sorted by citations
Riquelme, M., Aguirre, J., Bartnicki-Garcı́a, S., Braus, G. H., Feldbrügge, M., Fleig, U. N., Hansberg, W., Herrera‐Estrella, A., Kämper, J., Kück, U., et al. (2018) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00068-17 · Cited by 403
Self-citationWillger, S. D., Puttikamonkul, S., Kim, K.-H., Burritt, J. B., Grahl, N., Metzler, L. J., Barbuch, R., Bard, M., Lawrence, C. B., & Cramer, R. A. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000200 · Cited by 345
Fischer, R., Zekert, N., & Takeshita, N. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06193.x · Cited by 220
Self-citationAntifungal peptides: To be or not to be membrane active
Rautenbach, M., Troskie, A. M., & Vosloo, J. A. (2016) · Biochimie · doi:10.1016/j.biochi.2016.05.013 · Cited by 213
Membrane Microdomains, Rafts, and Detergent-Resistant Membranes in Plants and Fungi
Malínský, J., Opekarová, M., Großmann, G., & Tanner, W. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120103 · Cited by 190
Metadata
- Authors
- Takeshita, N., Higashitsuji, Y., Konzack, S., & Fischer, R.
- Affiliations
- Karlsruhe Institute of Technology (DE)
- Applied Microbiology, University of Karlsruhe, D-76187 Karlsruhe, Germany
- ORCID
- Takeshita, Norio
- Venue
- Molecular Biology of the Cell
- Published
- 2008-01
- Volume
- 19
- Issue
- 1
- Pages
- 339-351
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC2174190
- OpenAlex
- W2137246929
- Cited by
- 163
- References
- 58
- Citations by year
- 2026: 9 · 2025: 6 · 2024: 8 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 12 · 2018: 6 · 2017: 13 · 2016: 12 · 2015: 14 · 2014: 13 · 2013: 6 · 2012: 4
- FWCI
- 3.84
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- Aspergillus nidulans, hyphal tip, polarized growth, tear, Spitzenkörper, vesicle trafficking, filipin, hyphal morphology
- MeSH terms
- Aspergillus nidulans, Cell Membrane, Filipin, Fungal Proteins, Microtubules, Phenotype, Protein Binding, Sterols, Biomarkers, Cell Polarity, Gene Deletion, Protein Transport, Hyphae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
