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Endocytic Accessory Proteins Are Functionally Distinguished by Their Differential Effects on the Maturation of Clathrin-coated Pits

Mettlen, Marcel, Stoeber, Miriam, Loerke, Dinah, Antonescu, Costin N., Danuser, Gaudenz, Schmid, Sandra L.·2009·Molecular Biology of the Cell
DOI 10.1091/mbc.e09-03-0256
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Abstract

Diverse cargo molecules (i.e., receptors and ligand/receptor complexes) are taken into the cell by clathrin-mediated endocytosis (CME) utilizing a core machinery consisting of cargo-specific adaptors, clathrin and the GTPase dynamin. Numerous endocytic accessory proteins are also required, but their differential roles and functional hierarchy during CME are not yet understood. Here, we used a combination of quantitative live-cell imaging by total internal reflection fluorescence microscopy (TIR-FM), and decomposition of the lifetime distributions of clathrin-coated pits (CCPs) to measure independent aspects of CCP dynamics, including the turnover of abortive and productive CCP species and their relative contributions. Capitalizing on the sensitivity of this assay, we have examined the effects of specific siRNA-mediated depletion of endocytic accessory proteins on CME progression. Of the 12 endocytic accessory proteins examined, we observed seven qualitatively different phenotypes upon protein depletion. From this data we derive a temporal hierarchy of protein function during early steps of CME. Our results support the idea that a subset of accessory proteins, which mediate coat assembly, membrane curvature, and cargo selection, can provide input into an endocytic restriction point/checkpoint mechanism that monitors CCP maturation.

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  • Incorporation of transferrin receptor/AP2 into CCPs increases the efficiency of CCP maturation without affecting CCP density or CCP maturation rate.

    “The incorporation of certain cargo/adaptor protein complexes (i.e., transferrin receptor/AP2) into CCPs increases the efficiency of CCP maturation without affecting CCP density or CCP maturation rate (Loerke et al., 2009; Mettlen et al., 2009).”

    From Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis (2010) · cites this paper as Mettlen et al., 2009

  • We identified a subset of endocytic accessory factors, including membrane curvature and coat-sensing proteins, whose siRNA knockdown altered the rate and extent of turnover of abortive CCPs.

    “Subsequently, we identified a subset of endocytic accessory factors, including membrane curvature and coat-sensing proteins, whose siRNA knockdown altered the rate and extent of turnover of abortive CCPs (Mettlen et al., 2009).”

    From Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis (2010) · cites this paper as Mettlen et al., 2009

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Research works printed but not linked: 58

  1. Benmerah A., 1999, J. Cell Sci, 112, 1303, 10.1242/jcs.112.9.1303 · DOI
  2. Brodsky F. M., 2001, Annu. Rev. Cell Dev. Biol, 17, 517, 10.1146/annurev.cellbio.17.1.517 · DOI
  3. Carbone R., 1997, Cancer Res, 57, 5498
  4. Chen C., 2008, Proc. Natl. Acad. Sci. USA, 105, 11790, 10.1073/pnas.0803711105 · DOI · Paper page
  5. Chen H., 1998, Nature, 394, 793, 10.1038/29555 · DOI · Paper page
  6. Chetrit D., 2009, Biochem. J, 418, 701, 10.1042/BJ20081288 · DOI · Paper page
  7. Conner S. D., 2002, J. Cell Biol, 156, 921, 10.1083/jcb.200108123 · DOI
  8. Conner S. D., 2003, Nature, 422, 37, 10.1038/nature01451 · DOI
  9. Ehrlich M., 2004, Cell, 118, 591, 10.1016/j.cell.2004.08.017 · DOI · Paper page
  10. 10.1091/mbc.e03-09-0639 · DOI
  11. Gaidarov I., 1999, Nat. Cell Biol, 1, 1, 10.1038/8971 · DOI
  12. Gallop J. L., 2006, EMBO J, 25, 2898, 10.1038/sj.emboj.7601174 · DOI
  13. Guipponi M., 1998, Genomics, 53, 369, 10.1006/geno.1998.5521 · DOI
  14. Harel A., 2008, Traffic, 9, 417, 10.1111/j.1600-0854.2007.00694.x · DOI · Paper page
  15. Hawryluk M. J., 2006, Traffic, 7, 262, 10.1111/j.1600-0854.2006.00383.x · DOI
  16. Hinrichsen L., 2006, Proc. Natl. Acad. Sci. USA, 103, 8715, 10.1073/pnas.0600312103 · DOI
  17. Howard L., 1999, J. Biol. Chem, 274, 31693, 10.1074/jbc.274.44.31693 · DOI
  18. Huang F., 2004, J. Biol. Chem, 279, 16657, 10.1074/jbc.C400046200 · DOI
  19. Hussain N. K., 2001, Nat. Cell Biol, 3, 927, 10.1038/ncb1001-927 · DOI
  20. Itoh T., 2006, Biochim. Biophys. Acta, 1761, 897, 10.1016/j.bbalip.2006.06.015 · DOI · Paper page
  21. Jaqaman K., 2008, Nat. Methods, 5, 695, 10.1038/nmeth.1237 · DOI
  22. Jiang R., 2000, J. Biol. Chem, 275, 8439, 10.1074/jbc.275.12.8439 · DOI
  23. Kaksonen M., 2003, Cell, 115, 475, 10.1016/S0092-8674(03)00883-3 · DOI · Paper page
  24. Kazazic M., 2009, Traffic, 10, 235, 10.1111/j.1600-0854.2008.00858.x · DOI
  25. Keyel P. A., 2004, J. Biol. Chem, 279, 13190, 10.1074/jbc.M312717200 · DOI
  26. Le Clainche C., 2007, EMBO J, 26, 1199, 10.1038/sj.emboj.7601576 · DOI
  27. Lee D. W., 2006, J. Cell Sci, 119, 3502, 10.1242/jcs.03092 · DOI
  28. Lee D. W., 2005, J. Cell Sci, 118, 4311, 10.1242/jcs.02548 · DOI
  29. Loerke D., 2009, PLoS. Biol, 7, e57, 10.1371/journal.pbio.1000057 · DOI · Paper page
  30. Lundmark R., 2009, J. Cell Sci, 122, 5, 10.1242/jcs.037135 · DOI
  31. Massol R. H., 2006, Proc. Natl. Acad. Sci. USA, 103, 10265, 10.1073/pnas.0603369103 · DOI
  32. McGavin M. K., 2001, J. Exp. Med, 194, 1777, 10.1084/jem.194.12.1777 · DOI
  33. McPherson P. S., 2002, Trends Cell Biol, 12, 312, 10.1016/S0962-8924(02)02309-7 · DOI
  34. Merrifield C. J., 2002, Nat. Cell Biol, 4, 691, 10.1038/ncb837 · DOI · Paper page
  35. Meyerholz A., 2005, Traffic, 6, 1225, 10.1111/j.1600-0854.2005.00355.x · DOI
  36. Miliaras N. B., 2004, Cell Biochem. Biophys, 41, 295, 10.1385/CBB:41:2:295 · DOI
  37. Miller K., 1991, Cell, 65, 621, 10.1016/0092-8674(91)90094-F · DOI
  38. Nakashima S., 1999, EMBO J, 18, 3629, 10.1093/emboj/18.13.3629 · DOI · Paper page
  39. Newmyer S. L., 2003, Dev. Cell, 4, 929, 10.1016/S1534-5807(03)00157-6 · DOI
  40. Okamoto M., 1999, Biol. Chem, 274, 18446, 10.1074/jbc.274.26.18446 · DOI
  41. Pucadyil T. J., 2008, Cell, 135, 1263, 10.1016/j.cell.2008.11.020 · DOI
  42. Rappoport J. Z., 2008, PLoS. ONE, 3, e2416, 10.1371/journal.pone.0002416 · DOI
  43. Ringstad N., 1999, Neuron, 24, 143, 10.1016/S0896-6273(00)80828-4 · DOI
  44. Schmid E. M., 2007, Nature, 448, 883, 10.1038/nature06031 · DOI · Paper page
  45. Sengar A. S., 1999, EMBO J, 18, 1159, 10.1093/emboj/18.5.1159 · DOI
  46. 10.1091/mbc.e04-11-1016 · DOI
  47. Stahelin R. V., 2003, J. Biol. Chem, 278, 28993, 10.1074/jbc.M302865200 · DOI
  48. Thomson D. J., 1991, Advances in Spectrum Analysis and Array Processing, 58
  49. Umeda A., 2000, Eur. J. Cell Biol, 79, 336, 10.1078/S0171-9335(04)70037-0 · DOI
  50. Ungewickell E., 1995, Nature, 378, 632, 10.1038/378632a0 · DOI
  51. Ungewickell E. J., 2007, Curr. Opin. Cell Biol, 19, 417, 10.1016/j.ceb.2007.05.003 · DOI
  52. Wang M. Q., 2003, J. Biol, 3, 4, 10.1186/1475-4924-3-4 · DOI
  53. Wilbur J. D., 2008, J. Biol. Chem, 283, 32870, 10.1074/jbc.M802863200 · DOI
  54. Yamabhai M., 1998, J. Biol. Chem, 273, 31401, 10.1074/jbc.273.47.31401 · DOI · Paper page
  55. Yarar D., 2008, Traffic, 9, 133, 10.1111/j.1600-0854.2007.00675.x · DOI
  56. 10.1091/mbc.e04-09-0774 · DOI · Paper page
  57. Yarar D., 2007, Dev. Cell, 13, 43, 10.1016/j.devcel.2007.04.014 · DOI
  58. Zhang C. X., 2005, Traffic, 6, 1103, 10.1111/j.1600-0854.2005.00346.x · DOI

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Metadata

Authors
Mettlen, M., Stoeber, M., Loerke, D., Antonescu, C. N., Danuser, G., & Schmid, S. L.
Affiliations
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2009-07-15
Volume
20
Issue
14
Pages
3251-3260
ISSN
  • 1059-1524
  • 1939-4586
DOI
10.1091/mbc.e09-03-0256
Cited by
119
References
2
Language
en

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