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A Dynamic Actin Cytoskeleton Functions at Multiple Stages of Clathrin-mediated Endocytosis

Yarar, Defne, Waterman-Storer, Clare M., Schmid, Sandra L.·2005·Molecular Biology of the Cell
DOI 10.1091/mbc.e04-09-0774
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Abstract

Clathrin-mediated endocytosis in mammalian cells is critical for a variety of cellular processes including nutrient uptake and cell surface receptor down-regulation. Despite the findings that numerous endocytic accessory proteins directly or indirectly regulate actin dynamics and that actin assembly is spatially and temporally coordinated with endocytosis, direct functional evidence for a role of actin during clathrin-coated vesicle formation is lacking. Here, we take parallel biochemical and microscopic approaches to address the contribution of actin polymerization/depolymerization dynamics to clathrin-mediated endocytosis. When measured using live-cell fluorescence microscopy, disruption of the F-actin assembly and disassembly cycle with latrunculin A or jasplakinolide results in near complete cessation of all aspects of clathrin-coated structure (CCS) dynamics. Stage-specific biochemical assays and quantitative fluorescence and electron microscopic analyses establish that F-actin dynamics are required for multiple distinct stages of clathrin-coated vesicle formation, including coated pit formation, constriction, and internalization. In addition, F-actin dynamics are required for observed diverse CCS behaviors, including splitting of CCSs from larger CCSs, merging of CCSs, and lateral mobility on the cell surface. Our results demonstrate a key role for actin during clathrin-mediated endocytosis in mammalian cells.

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  • The observed heterogeneity in the dynamic behavior of CCPs could reflect differences in their content of cargo and adaptor molecules.

    “Together, these data strongly suggest that the observed heterogeneity in the dynamic behavior of CCPs (Merrifield et al., 2005; Rappoport et al., 2005; Yarar et al., 2005) could reflect differences in their content of cargo and adaptor molecules.”

    From Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis (2010) · cites this paper as Yarar et al., 2005

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Metadata

Authors
Yarar, D., Waterman-Storer, C. M., & Schmid, S. L.
Affiliations
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2005-02
Volume
16
Issue
2
Pages
964-975
ISSN
  • 1059-1524
  • 1939-4586
DOI
10.1091/mbc.e04-09-0774
Cited by
408
References
3
Language
en

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