CoChat
Sign Up
Cited by 69

The DOI matches its Crossref record.

Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function

Reverter, Meritxell, Rentero, Carles, de Muga, Sandra Vilà, Alvarez-Guaita, Anna, Mulay, Vishwaroop, Cairns, Rose, Wood, Peta, Monastyrskaya, Katia, Pol, Albert, Tebar, Francesc, Blasi, Joan, Grewal, Thomas, Enrich, Carlos·2011·Molecular Biology of the Cell
DOI 10.1091/mbc.e11-04-0332r
Open this paper in CoChat — free account

Abstract

Cholesterol regulates plasma membrane (PM) association and functioning of syntaxin-4 and soluble N-ethylmaleimide-sensitive fusion protein 23 (SNAP23) in the secretory pathway. However, the molecular mechanism and cellular cholesterol pools that determine the localization and assembly of these target membrane SNAP receptors (t-SNAREs) are largely unknown. We recently demonstrated that high levels of annexin A6 (AnxA6) induce accumulation of cholesterol in late endosomes, thereby reducing cholesterol in the Golgi and PM. This leads to an impaired supply of cholesterol needed for cytosolic phospholipase A 2(cPLA 2) to drive Golgi vesiculation and caveolin transport to the cell surface. Using AnxA6-overexpressing cells as a model for cellular cholesterol imbalance, we identify impaired cholesterol egress from late endosomes and diminution of Golgi cholesterol as correlating with the sequestration of SNAP23/syntaxin-4 in Golgi membranes. Pharmacological accumulation of late endosomal cholesterol and cPLA 2 inhibition induces a similar phenotype in control cells with low AnxA6 levels. Ectopic expression of Niemann-Pick C1 (NPC1) or exogenous cholesterol restores the location of SNAP23 and syntaxin-4 within the PM. Importantly, AnxA6-mediated mislocalization of these t-SNAREs correlates with reduced secretion of cargo via the SNAP23/syntaxin-4–dependent constitutive exocytic pathway. We thus conclude that inhibition of late endosomal export and Golgi cholesterol depletion modulate t-SNARE localization and functioning along the exocytic pathway.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.e11-04-0332r

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In vitro, ACAT inhibition has cell-specific effects on ABCA1 and cholesterol efflux: it increases ABCA1 levels and cholesterol efflux in cholesterol-loaded macrophages, decreases ABCA1 levels in adipocytes, and decreases ABCA1 levels, lipid catabolism, and cholesterol efflux in APOE4 microglia.

    “In vitro, ACAT inhibition increases ABCA1 levels and cholesterol efflux of cholesterol-loaded macrophages, while decreasing ABCA1 levels in adipocytes [125] and decreasing ABCA1 levels, lipid catabolism, and cholesterol efflux in APOE4 microglia [126–128]”

    From Inhibition of ACAT as a Therapeutic Target for Alzheimer's Disease Is Independent of ApoE4 Lipidation (2023) · cites this paper as [125]

    Split into 3 atoms — 0 confirmed, 0 partially supported, 0 disputed, 0 misattributed, 3 unverifiable. Expand the claim to see per-atom verdicts.

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 89

  1. Aggeler J, 1982, Exp Cell Res, 139, 275, 10.1016/0014-4827(82)90252-X · DOI
  2. Alpy F, 2005, J Cell Sci, 118, 2791, 10.1242/jcs.02485 · DOI
  3. Avery J, 2000, J Cell Biol, 148, 317, 10.1083/jcb.148.2.317 · DOI
  4. Ayala-Sanmartin J, 2001, Biochem Biophys Res Commun, 283, 72, 10.1006/bbrc.2001.4748 · DOI
  5. Babiychuk EB, 2008, Traffic, 9, 1757, 10.1111/j.1600-0854.2008.00800.x · DOI
  6. Band AM, 2002, FEBS Lett, 531, 513, 10.1016/S0014-5793(02)03605-0 · DOI
  7. Bradford MM, 1976, Anal Biochem, 72, 248, 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  8. Bryant NJ, 2002, Nat Rev Mol Cell Biol, 3, 267, 10.1038/nrm782 · DOI · Paper page
  9. Chamberlain LH, 2001, Proc Natl Acad Sci USA, 98, 5619, 10.1073/pnas.091502398 · DOI
  10. Choudhury A, 2006, Nat Cell Biol, 8, 317, 10.1038/ncb1380 · DOI
  11. Connell E, 2007, EMBO Rep, 8, 414, 10.1038/sj.embor.7400935 · DOI
  12. Creutz CE, 1992, Science, 258, 924, 10.1126/science.1439804 · DOI
  13. Cubells L, 2008, J Biol Chem, 283, 10174, 10.1074/jbc.M706618200 · DOI
  14. Cubells L, 2007, Traffic, 8, 1568, 10.1111/j.1600-0854.2007.00640.x · DOI
  15. de Diego I, 2002, J Biol Chem, 277, 32187, 10.1074/jbc.M205499200 · DOI
  16. Domon MM, 2010, J Colloid Interface Sci, 346, 436, 10.1016/j.jcis.2010.03.015 · DOI
  17. Donnelly SR, 1997, Cell Mol Life Sci, 53, 533, 10.1007/s000180050068 · DOI
  18. Du X, 2011, J Cell Biol, 192, 121, 10.1083/jcb.201004142 · DOI
  19. Enrich C, 2011, Biochim Biophys Acta, 1813, 935, 10.1016/j.bbamcr.2010.09.015 · DOI
  20. Fiedler K, 1995, J Cell Biol, 128, 1043, 10.1083/jcb.128.6.1043 · DOI
  21. Foster LJ, 2003, Proc Natl Acad Sci USA, 100, 5813, 10.1073/pnas.0631608100 · DOI
  22. Garver WS, 2002, Curr Mol Med, 2, 485, 10.2174/1566524023362375 · DOI
  23. Gerke V, 2005, Nat Rev Mol Cell Biol, 6, 449, 10.1038/nrm1661 · DOI
  24. Gerke V, 2002, Physiol Rev, 82, 331, 10.1152/physrev.00030.2001 · DOI · Paper page
  25. 10.1091/mbc.e08-04-0383 · DOI
  26. Grewal T, 2006, Bioessays, 28, 1211, 10.1002/bies.20503 · DOI
  27. Grewal T, 2009, Cell Signal, 21, 847, 10.1016/j.cellsig.2009.01.031 · DOI
  28. Grewal T, 2005, Oncogene, 24, 5809, 10.1038/sj.onc.1208743 · DOI
  29. Grewal T, 2000, J Biol Chem, 275, 33806, 10.1074/jbc.M002662200 · DOI
  30. Grewal T, 2010, Int J Biochem Cell Biol, 42, 580, 10.1016/j.biocel.2009.12.020 · DOI
  31. Grimmer S, 2005, Traffic, 6, 144, 10.1111/j.1600-0854.2005.00258.x · DOI
  32. Hong W, 2005, Biochim Biophys Acta, 1744, 493
  33. Ikonen E, 2008, Nat Rev Mol Cell Biol, 9, 125, 10.1038/nrm2336 · DOI
  34. Jackle S, 1994, J Biol Chem, 269, 1026, 10.1016/S0021-9258(17)42215-0 · DOI
  35. Kamal A, 1998, J Cell Biol, 142, 937, 10.1083/jcb.142.4.937 · DOI
  36. Kay JG, 2006, J Biol Chem, 281, 11949, 10.1074/jbc.M600857200 · DOI
  37. Lafont F, 1999, Proc Natl Acad Sci USA, 96, 3734, 10.1073/pnas.96.7.3734 · DOI
  38. Lang T, 2007, J Physiol, 585, 693, 10.1113/jphysiol.2007.134346 · DOI
  39. Lang T, 2001, EMBO J, 20, 2202, 10.1093/emboj/20.9.2202 · DOI
  40. Latham CF, 2007, Int J Biochem Cell Biol, 39, 1576, 10.1016/j.biocel.2006.11.015 · DOI
  41. Lemmon MA, 2008, Nat Rev Mol Cell Biol, 9, 99, 10.1038/nrm2328 · DOI · Paper page
  42. Leung SM, 1998, J Biol Chem, 273, 17732, 10.1074/jbc.273.28.17732 · DOI
  43. Low SH, 1998, J Cell Biol, 141, 1503, 10.1083/jcb.141.7.1503 · DOI
  44. 10.1091/mbc.e05-05-0462 · DOI
  45. Lowry OH, 1951, J Biol Chem, 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  46. Manderson AP, 2007, J Cell Biol, 178, 57, 10.1083/jcb.200612131 · DOI · Paper page
  47. Martin LB, 1998, J Biol Chem, 273, 1444, 10.1074/jbc.273.3.1444 · DOI
  48. Martin-Martin B, 2000, Blood, 96, 2574, 10.1182/blood.V96.7.2574 · DOI
  49. Matsushita K, 2003, Cell, 115, 139, 10.1016/S0092-8674(03)00803-1 · DOI
  50. Maxfield FR, 2005, Nature, 438, 612, 10.1038/nature04399 · DOI
  51. Mayran N, 2003, EMBO J, 22, 3242, 10.1093/emboj/cdg321 · DOI
  52. Monastyrskaya K, 2009, Cell Mol Life Sci, 66, 2623, 10.1007/s00018-009-0027-1 · DOI
  53. Monastyrskaya K, 2009, J Biol Chem, 284, 17227, 10.1074/jbc.M109.004457 · DOI
  54. Morel E, 2009, Dev Cell, 16, 445, 10.1016/j.devcel.2009.01.007 · DOI
  55. Neufeld EB, 1996, J Biol Chem, 271, 21604, 10.1074/jbc.271.35.21604 · DOI
  56. Ngo MH, 2010, Biochem J, 429, 13, 10.1042/BJ20100263 · DOI
  57. Okayama M, 2007, FEBS Lett, 581, 4583, 10.1016/j.febslet.2007.08.046 · DOI
  58. Oliferenko S, 1999, J Cell Biol, 146, 843, 10.1083/jcb.146.4.843 · DOI
  59. Podszywalow-Bartnicka P, 2010, J Cell Biochem, 111, 168, 10.1002/jcb.22685 · DOI
  60. 10.1091/mbc.e04-08-0737 · DOI
  61. Pol A, 1997, Biochem J, 327, 741, 10.1042/bj3270741 · DOI
  62. Pons M, 2001, Exp Cell Res, 269, 13, 10.1006/excr.2001.5268 · DOI
  63. Pons M, 2000, Exp Cell Res, 257, 33, 10.1006/excr.2000.4861 · DOI
  64. Predescu SA, 2005, J Biol Chem, 280, 37130, 10.1074/jbc.M505659200 · DOI
  65. Pulido IR, 2010, Biochim Biophys Acta, 1813, 1038, 10.1016/j.bbamcr.2010.11.007 · DOI
  66. Puri N, 2006, Traffic, 7, 1482, 10.1111/j.1600-0854.2006.00490.x · DOI
  67. Rao SK, 2004, J Biol Chem, 279, 20471, 10.1074/jbc.M400798200 · DOI · Paper page
  68. Raptis A, 2005, J Chem Neuroanat, 30, 201, 10.1016/j.jchemneu.2005.08.002 · DOI
  69. Rasband WS, 1997, ImageJ
  70. Rentero C, 2006, Cell Signal, 18, 1006, 10.1016/j.cellsig.2005.08.008 · DOI
  71. Rickman C, 2005, Chem Biol, 12, 545, 10.1016/j.chembiol.2005.03.004 · DOI
  72. Salaun C, 2005, J Biol Chem, 280, 19449, 10.1074/jbc.M501923200 · DOI
  73. Salaun C, 2004, Traffic, 5, 255, 10.1111/j.1600-0854.2004.0162.x · DOI
  74. Schnitzer JE, 1995, J Biol Chem, 270, 14399, 10.1074/jbc.270.24.14399 · DOI
  75. Sieber JJ, 2006, Biophys J, 90, 2843, 10.1529/biophysj.105.079574 · DOI
  76. Smart EJ, 2004, Proc Natl Acad Sci USA, 101, 3450, 10.1073/pnas.0400441101 · DOI
  77. Smythe E, 1994, J Cell Biol, 124, 301, 10.1083/jcb.124.3.301 · DOI
  78. Soccio RE, 2003, J Biol Chem, 278, 22183, 10.1074/jbc.R300003200 · DOI · Paper page
  79. St-Denis JF, 1999, Biochem J, 338, 709, 10.1042/bj3380709 · DOI
  80. Sudhof TC, 2009, Science, 323, 474, 10.1126/science.1161748 · DOI · Paper page
  81. Sztolsztener ME, 2010, Arch Biochem Biophys, 493, 221, 10.1016/j.abb.2009.11.001 · DOI
  82. Torrejon-Escribano B, 2002, FEBS Lett, 512, 275, 10.1016/S0014-5793(02)02278-0 · DOI
  83. Umbrecht-Jenck E, 2010, Traffic, 11, 958, 10.1111/j.1600-0854.2010.01065.x · DOI
  84. Urano Y, 2008, Proc Natl Acad Sci USA, 105, 16513, 10.1073/pnas.0807450105 · DOI
  85. Vila de Muga S, 2009, Oncogene, 28, 363, 10.1038/onc.2008.386 · DOI
  86. Wang P, 2007, Am J Respir Cell Mol Biol, 37, 467, 10.1165/rcmb.2006-0447OC · DOI
  87. Wang Y, 2000, Traffic, 1, 952, 10.1034/j.1600-0854.2000.011205.x · DOI
  88. Wendler F, 2001, Traffic, 2, 606, 10.1034/j.1600-0854.2001.20903.x · DOI
  89. Wojtanik KM, 2003, J Biol Chem, 278, 14850, 10.1074/jbc.M300488200 · DOI

Cited by

Sorted by citations

Metadata

Authors
Reverter, M., Rentero, C., de Muga, S. V., Alvarez-Guaita, A., Mulay, V., Cairns, R., Wood, P., Monastyrskaya, K., Pol, A., Tebar, F., Blasi, J., Grewal, T., & Enrich, C.
Affiliations
  • Departament de Biologia Cel·lular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain
  • Faculty of Pharmacy, University of Sydney, Sydney, NSW 2006, Australia
  • Urology Research Laboratory, Department of Clinical Research, University of Bern, 3000 Bern 9, Switzerland
  • Department of Pathology and Experimental Therapeutics, IDIBELL–University of Barcelona, 08907 L'Hospitalet de Llobregat, Barcelona, Spain
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2011-11
Volume
22
Issue
21
Pages
4108-4123
ISSN
  • 1059-1524
  • 1939-4586
DOI
10.1091/mbc.e11-04-0332r
Cited by
69
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review