The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Three-dimensional ultrastructure of the septin filament network in Saccharomyces cerevisiae
Open accessAbstract
Septins are conserved GTP-binding proteins involved in membrane compartmentalization and remodeling. In budding yeast, five mitotic septins localize at the bud neck, where the plasma membrane is enriched in phosphatidylinositol-4,5-bisphosphate (PtdIns4,5P(2)). We previously established the subunit organization within purified yeast septin complexes and how these hetero-octamers polymerize into filaments in solution and on PtdIns4,5P(2)-containing lipid monolayers. How septin ultrastructure in vitro relates to the septin-containing filaments observed at the neck in fixed cells by thin-section electron microscopy was unclear. A morphological description of these filaments in the crowded space of the cell is challenging, given their small cross section. To examine septin organization in situ, sections of dividing yeast cells were analyzed by electron tomography of freeze-substituted cells, as well as by cryo-electron tomography. We found networks of filaments both perpendicular and parallel to the mother-bud axis that resemble septin arrays on lipid monolayers, displaying a repeat pattern that mirrors the molecular dimensions of the corresponding septin preparations in vitro. Thus these in situ structures most likely represent septin filaments. In viable mutants lacking a single septin, in situ filaments are still present, although more disordered, consistent with other evidence that the in vivo function of septins requires filament formation.
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In the citing paper
“Septins form short filaments that self-assemble into parallel arrays which are non-polar in nature [50, 57, 58].”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [57]
In the citing paper
“Since the Septin heterotirmeric subunits align parallel to each other within the ordered Septin structures that form during mitosis [57–59], there must be lateral interactions between the fibers that stabilize their lattice like structure.”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [57]
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Citations
Cites
Computer Visualization of Three-Dimensional Image Data Using IMOD
Kremer, J. R., Mastronarde, D. N., & McIntosh, J. R. (1996) · Journal of Structural Biology · doi:10.1006/jsbi.1996.0013 · Cited by 5,530
Genetic Control of the Cell Division Cycle in Yeast
Hartwell, L. H., Culotti, J. G., Pringle, J. R., & Reid, B. J. (1974) · Science · doi:10.1126/science.183.4120.46 · Cited by 1,567
Saccharomyces cerevisiae cell cycle
Hartwell, L. H. (1974) · Bacteriological Reviews · doi:10.1128/br.38.2.164-198.1974 · Cited by 723
HARTWELL, L. (1971) · Experimental Cell Research · doi:10.1016/0014-4827(71)90223-0 · Cited by 650
amira: A Highly Interactive System for Visual Data Analysis
Stalling, D., Westerhoff, M., & Hege, H. (2005) · Elsevier eBooks · doi:10.1016/b978-012387582-2/50040-x · Cited by 570
Structural insight into filament formation by mammalian septins
Sirajuddin, M., Farkasovsky, M., Hauer, F., Kühlmann, D., Macara, I. G., Weyand, M., Stark, H., & Wittinghofer, A. (2007) · Nature · doi:10.1038/nature06052 · Cited by 481
Cryo‐electron microscopy of vitreous sections
Al‐Amoudi, A., Chang, J.-J., Leforestier, A., McDowall, A. W., Michel, L. S., Norlen, L. P. O., Richter, K., Blanc, N. S., Studer, D., & Dubochet, J. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600366 · Cited by 458
Takizawa, P. A., DeRisi, J. L., Wilhelm, J. E., & Vale, R. D. (2000) · Science · doi:10.1126/science.290.5490.341 · Cited by 440
Noise Reduction in Electron Tomographic Reconstructions Using Nonlinear Anisotropic Diffusion
Frangakis, A. S., & Hegerl, R. (2001) · Journal of Structural Biology · doi:10.1006/jsbi.2001.4406 · Cited by 425
A highly ordered ring of membrane-associated filaments in budding yeast.
Byers, B., & Goetsch, L. (1976) · The Journal of Cell Biology · doi:10.1083/jcb.69.3.717 · Cited by 420
Flagged first, then by citations
Research works printed but not linked: 3
- Garcia G, 2011, J Cell Biol
- McMurray MA, 2008, The Septins, 49
- Pringle JR, 2008, The Septins, 7
Cited by
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Items 101–106 of 106
In situ cryo-electron tomography of vaccinia virus exit from infected cellsPreprint
Hernández‐González, M., Nans, A., Calcraft, T., Rosenthal, P. B., & Way, M. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.08.20.671218
Regulation of fission yeast cytokinesis by membrane lipids and septins
Arbizzani, F. (2019) · theses.fr (ABES) · doi:10.70675/7a1732e6z2bf4z4d28z8434za0d0b449ce1f
Mechanistic insights into the role of septins in animal cell morphogenesis
Martins, C. S. (2022) · theses.fr (ABES) · doi:10.70675/64a5a43bzf7cez414ezb866z652c1414b21d
Mechanical Properties of Septins and Their Role in Establishing Diffusion Barriers
Chauvin, B. (2022) · theses.fr (ABES) · doi:10.70675/3d3cb268z6531z496dz8ebcz9c81b8f85871
Adriaans, I. E., Ibanes, S., Bertin, A., Cazevieille, C., Lai‐Kee‐Him, J., Geymonat, M., Picas, L., & Piatti, S. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.117862
Self-citation
Metadata
- Authors
- Bertin, A., McMurray, M. A., Pierson, J., Thai, L., McDonald, K. L., Zehr, E. A., García, G., Peters, P., Thorner, J., & Nogales, E.
- Affiliations
- University of California, Berkeley (US)
- The Netherlands Cancer Institute (NL)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Nogales, Eva
- ORCID
- Venue
- Molecular Biology of the Cell
- Published
- 2012-02
- Volume
- 23
- Issue
- 3
- Pages
- 423-432
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC3268722
- OpenAlex
- W2013196716
- Cited by
- 106
- References
- 64
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 5 · 2023: 5 · 2022: 5 · 2021: 10 · 2020: 8 · 2019: 3 · 2018: 8 · 2017: 4 · 2016: 14 · 2015: 12 · 2014: 9 · 2013: 7 · 2012: 6
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of General Medical Sciences (R00GM086603, K99 GM86603, R01GM21841, R01GM101314, K99GM086603, R01GM021841)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- septins, Saccharomyces cerevisiae, bud neck, membrane compartmentalization, electron tomography, cryo-electron tomography, thin section electron microscopy, filament polymerization
- MeSH terms
- Cytoskeleton, Mutation, Saccharomyces cerevisiae, Imaging, Three-Dimensional, Saccharomyces cerevisiae Proteins, Septins
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