The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Lipid droplet autophagy in the yeast Saccharomyces cerevisiae
Open accessAbstract
Cytosolic lipid droplets (LDs) are ubiquitous organelles in prokaryotes and eukaryotes that play a key role in cellular and organismal lipid homeostasis. Triacylglycerols (TAGs) and steryl esters, which are stored in LDs, are typically mobilized in growing cells or upon hormonal stimulation by LD-associated lipases and steryl ester hydrolases. Here we show that in the yeast Saccharomyces cerevisiae, LDs can also be turned over in vacuoles/lysosomes by a process that morphologically resembles microautophagy. A distinct set of proteins involved in LD autophagy is identified, which includes the core autophagic machinery but not Atg11 or Atg20. Thus LD autophagy is distinct from endoplasmic reticulum–autophagy, pexophagy, or mitophagy, despite the close association between these organelles. Atg15 is responsible for TAG breakdown in vacuoles and is required to support growth when de novo fatty acid synthesis is compromised. Furthermore, none of the core autophagy proteins, including Atg1 and Atg8, is required for LD formation in yeast.
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Cites
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MethodAutophagy regulates lipid metabolism
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Plasma membrane contributes to the formation of pre-autophagosomal structures
Ravikumar, B., Moreau, K., Jahreiss, L., Puri, C., & Rubinsztein, D. C. (2010) · Nature Cell Biology · doi:10.1038/ncb2078 · Cited by 902
Cargo recognition failure is responsible for inefficient autophagy in Huntington’s disease
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Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
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Autophagy Regulates Cholesterol Efflux from Macrophage Foam Cells via Lysosomal Acid Lipase
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An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
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Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
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A brief history of autophagy from cell biology to physiology and disease
Mizushima, N. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0092-5 · Cited by 696
Lipid droplet‐mediated lipid and protein homeostasis in budding yeast
Graef, M. (2018) · FEBS Letters · doi:10.1002/1873-3468.12996 · Cited by 65
Metadata
- Authors
- van Zutphen, T., Todde, V., de Boer, R., Kreim, M., Hofbauer, H. F., Wolinski, H., Veenhuis, M., van der Klei, I. J., & Kohlwein, S. D.
- Venue
- Molecular Biology of the Cell
- Published
- 2014-01-15
- Volume
- 25
- Issue
- 2
- Pages
- 290-301
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2099538852
- Cited by
- 291
- References
- 11
- FWCI
- 11.03
- Type
- journal article
- Language
- en
- Open access
- green
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