The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Lipid droplet autophagy in the yeast Saccharomyces cerevisiae
Open accessAbstract
Cytosolic lipid droplets (LDs) are ubiquitous organelles in prokaryotes and eukaryotes that play a key role in cellular and organismal lipid homeostasis. Triacylglycerols (TAGs) and steryl esters, which are stored in LDs, are typically mobilized in growing cells or upon hormonal stimulation by LD-associated lipases and steryl ester hydrolases. Here we show that in the yeast Saccharomyces cerevisiae, LDs can also be turned over in vacuoles/lysosomes by a process that morphologically resembles microautophagy. A distinct set of proteins involved in LD autophagy is identified, which includes the core autophagic machinery but not Atg11 or Atg20. Thus LD autophagy is distinct from endoplasmic reticulum–autophagy, pexophagy, or mitophagy, despite the close association between these organelles. Atg15 is responsible for TAG breakdown in vacuoles and is required to support growth when de novo fatty acid synthesis is compromised. Furthermore, none of the core autophagy proteins, including Atg1 and Atg8, is required for LD formation in yeast.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · cited 1×
“The kanamycin selection marker in strains expressing Faa4-GFP, Erg6-GFP, and Sec63-GFP from the O’Shea collection (Huh et al., 2003) was swapped for the clonNAT marker, selected for nourseothricin resistance, and subsequently used for synthetic genetic array technology (Tong and Boone, 2006).”
MethodAutophagy regulates lipid metabolism
Singh, R., Kaushik, S., Wang, Y., Xiang, Y., Novak, I., Komatsu, M., Tanaka, K., Cuervo, A. M., & Czaja, M. J. (2009) · Nature · doi:10.1038/nature07976 · Cited by 3,975
Rabinowitz, J. D., & White, E. (2010) · Science · doi:10.1126/science.1193497 · Cited by 1,982
Induction of autophagy by spermidine promotes longevity
Eisenberg, T., Knauer, H., Schauer, A., Büttner, S., Ruckenstuhl, C., Carmona-Gutierrez, D., Ring, J., Schroeder, S., Magnes, C., Antonacci, L., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb1975 · Cited by 1,680
Plasma membrane contributes to the formation of pre-autophagosomal structures
Ravikumar, B., Moreau, K., Jahreiss, L., Puri, C., & Rubinsztein, D. C. (2010) · Nature Cell Biology · doi:10.1038/ncb2078 · Cited by 902
Cargo recognition failure is responsible for inefficient autophagy in Huntington’s disease
Martinez-Vicente, M., Talloczy, Z., Wong, E., Tang, G., Koga, H., Kaushik, S., de Vries, R., Arias, E., Harris, S., Sulzer, D., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2528 · Cited by 885
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., & Ohsumi, Y. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.435 · Cited by 881
Autophagy Regulates Cholesterol Efflux from Macrophage Foam Cells via Lysosomal Acid Lipase
Ouimet, M., Franklin, V., Mak, E., Liao, X., Tabas, I., & Marcel, Y. L. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.03.023 · Cited by 774
Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores.
Zechner, R., Kienesberger, P. C., Haemmerle, G., Zimmermann, R., & Lass, A. (2009) · Journal of Lipid Research · doi:10.1194/jlr.r800031-jlr200 · Cited by 576
An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
Mari, M., Griffith, J., Rieter, E., Krishnappa, L., Klionsky, D. J., & Reggiori, F. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200912089 · Cited by 491
Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationCargo recognition and degradation by selective autophagy
Gatica, D., Lahiri, V., & Klionsky, D. J. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0037-z · Cited by 1,067
A brief history of autophagy from cell biology to physiology and disease
Mizushima, N. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0092-5 · Cited by 696
Lipid droplet‐mediated lipid and protein homeostasis in budding yeast
Graef, M. (2018) · FEBS Letters · doi:10.1002/1873-3468.12996 · Cited by 65
Acetyl-CoA carboxylase 1–dependent lipogenesis promotes autophagy downstream of AMPK
Gross, A. S., Zimmermann, A., Pendl, T., Schroeder, S., Schoenlechner, H., Knittelfelder, O., Lamplmayr, L., Santiso, A., Aufschnaiter, A., Waltenstorfer, D., et al. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.007020 · Cited by 46
Nonoyama, T., Nojima, D., Maeda, Y., Noda, M., Yoshino, T., Matsumoto, M., Bowler, C., & Tanaka, T. (2019) · Algal Research · doi:10.1016/j.algal.2019.101660 · Cited by 29
Geltinger, F., Tevini, J., Briza, P., Geiser, A., Bischof, J., Richter, K., Felder, T., & Rinnerthaler, M. (2020) · GeroScience · doi:10.1007/s11357-019-00103-0 · Cited by 26
Demine, S., Tejerina, S., Bihin, B., Thiry, M., Reddy, N., Renard, P., Raes, M., Jadot, M., & Arnould, T. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.25994 · Cited by 21
Yoo, K., Bhattacharya, S., Oliveira, N. K., Pereira de Sa, N., Matos, G. S., Del Poeta, M., & Fries, B. C. (2024) · mBio · doi:10.1128/mbio.01847-24 · Cited by 8
Metadata
- Authors
- van Zutphen, T., Todde, V., de Boer, R., Kreim, M., Hofbauer, H. F., Wolinski, H., Veenhuis, M., van der Klei, I. J., & Kohlwein, S. D.
- Venue
- Molecular Biology of the Cell
- Published
- 2014-01-15
- Volume
- 25
- Issue
- 2
- Pages
- 290-301
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2099538852
- Cited by
- 291
- References
- 11
- FWCI
- 11.03
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
