Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules
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Abstract
Doublet and triplet microtubules are essential and highly stable core structures of centrioles, basal bodies, cilia, and flagella. In contrast to dynamic cytoplasmic microtubules, their luminal surface is coated with regularly arranged microtubule inner proteins (MIPs). However, the protein composition and biological function(s) of MIPs remain poorly understood. Using genetic, biochemical, and imaging techniques, we identified Tetrahymena RIB72A and RIB72B proteins as ciliary MIPs. Fluorescence imaging of tagged RIB72A and RIB72B showed that both proteins colocalize to Tetrahymena cilia and basal bodies but assemble independently. Cryoelectron tomography of RIB72A and/or RIB72B knockout strains revealed major structural defects in the ciliary A-tubule involving MIP1, MIP4, and MIP6 structures. The defects of individual mutants were complementary in the double mutant. All mutants had reduced swimming speed and ciliary beat frequencies, and high-speed video imaging revealed abnormal highly curved cilia during power stroke. Our results show that RIB72A and RIB72B are crucial for the structural assembly of ciliary A-tubule MIPs and are important for proper ciliary motility.
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In Chlamydomonas or other unicellular eukaryotes, CFAP20, PACRG, CFAP45, CFAP52 and EFHC1/Rib72 are all needed to regulate ciliary beat patterns.
“Consistent with such a role, in Chlamydomonas or other unicellular eukaryotes, CFAP20, PACRG, CFAP45, CFAP52 and EFHC1/Rib72 are all needed to regulate ciliary beat patterns20,76–78.”
From The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision (2022) · cites this paper as [77]
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- Authors
- Stoddard, D., Zhao, Y., Bayless, B. A., Gui, L., Louka, P., Dave, D., Suryawanshi, S., Tomasi, R. F.-X., Dupuis-Williams, P., Baroud, C. N., Gaertig, J., Winey, M., & Nicastro, D.
- Affiliations
- Brandeis University (US)
- University of Colorado Boulder (US)
- University of California, Davis (US)
- The University of Texas Southwestern Medical Center (US)
- University of Georgia (US)
- Centre National de la Recherche Scientifique (FR)
- UMR-S 1174 Inserm, Universite Paris-Sud, 91405 Orsay Cedex, France
- Corresponding author
- Gaertig, Jacek
- Winey, Mark
- Nicastro, Daniela
- ORCID
- Venue
- Molecular Biology of the Cell
- Published
- 2018-10-15
- Volume
- 29
- Issue
- 21
- Pages
- 2566-2577
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC6254578
- OpenAlex
- W2950608721
- Cited by
- 53
- References
- 45
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 5 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 12 · 2018: 1
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by-nc-sa)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM074746, R01GM089912, R01GM127571, R01GM083122)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Keywords
- Tetrahymena, microtubule inner proteins, basal bodies, ciliary motility, ciliary beat frequency, ciliary ultrastructure, MIP-1, microtubule assembly, flagella
- MeSH terms
- Cilia, Fluorescence, Microtubule Proteins, Microtubules, Models, Biological, Mutation, Phagocytosis, Tetrahymena, Video Recording, Protozoan Proteins, Protein Subunits, Green Fluorescent Proteins, Axoneme, Gene Knockout Techniques
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