The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization
Open accessPublished version of a preprint
Abstract
Ninein (Nin) is a microtubule (MT) anchor at the subdistal appendages of mother centrioles and the pericentriolar material (PCM) of centrosomes that also functions to organize MTs at noncentrosomal MT-organizing centers (ncMTOCs). In humans, the NIN gene is mutated in Seckel syndrome, an inherited developmental disorder. Here, we dissect the protein domains involved in Nin’s localization and interactions with dynein and ensconsin (ens/MAP7) and show that the association with ens cooperatively regulates MT assembly in Drosophila fat body cells. We define domains of Nin responsible for its localization to the ncMTOC on the fat body cell nuclear surface, localization within the nucleus, and association with Dynein light intermediate chain (Dlic) and ens, respectively. We show that Nin’s association with ens synergistically regulates MT assembly. Together, these findings reveal novel features of Nin function and its regulation of a ncMTOC.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Varadi, M., Anyango, S., Deshpande, M., Nair, S., Natassia, C., Yordanova, G., Yuan, D., Stroe, O., Wood, G., Laydon, A., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkab1061 · Cited by 7,850
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Dietzl, G., Chen, D., Schnorrer, F., Su, K.-C., Barinova, Y., Fellner, M., Gasser, B., Kinsey, K., Oppel, S., Scheiblauer, S., et al. (2007) · Nature · doi:10.1038/nature05954 · Cited by 2,644
NLStradamus: a simple Hidden Markov Model for nuclear localization signal prediction
Nguyen Ba, A. N., Pogoutse, A., Provart, N., & Moses, A. M. (2009) · BMC Bioinformatics · doi:10.1186/1471-2105-10-202 · Cited by 703
The cytoplasmic dynein transport machinery and its many cargoes
Reck-Peterson, S. L., Redwine, W. B., Vale, R. D., & Carter, A. P. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0004-3 · Cited by 659
Assembly of centrosomal proteins and microtubule organization depends on PCM-1
Dammermann, A., & Merdes, A. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200204023 · Cited by 489
Asymmetric centrosome inheritance maintains neural progenitors in the neocortex
Wang, X., Tsai, J.-W., Imai, J. H., Lian, W.-N., Vallee, R. B., & Shi, S.-H. (2009) · Nature · doi:10.1038/nature08435 · Cited by 475
Simple rules for passive diffusion through the nuclear pore complex
Timney, B. L., Raveh, B., Mironska, R., Trivedi, J. M., Kim, S. J., Russel, D., Wente, S. R., Sali, A., & Rout, M. P. (2016) · Journal of Cell Biology · doi:10.1083/jcb.201601004 · Cited by 457
FlyBase: updates to the Drosophila genes and genomes database
Öztürk-Çolak, A., Marygold, S. J., Antonazzo, G., Attrill, H., Goutte-Gattat, D., Jenkins, V. K., Matthews, B. B., Millburn, G., dos Santos, G., Tabone, C. J., et al. (2024) · GENETICS · doi:10.1093/genetics/iyad211 · Cited by 412
Microtubule minus-end anchorage at centrosomal and non-centrosomal sites: the role of ninein
Mogensen, M. M., Malik, A., Piel, M., Bouckson-Castaing, V., & Bornens, M. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.17.3013 · Cited by 396
Research works printed but not linked: 2
- Kao LR, 2004, Methods Mol Biol, 247, 443
- Wainman A, 2012, J Cell Sci, 125, 4014
Metadata
- Authors
- Tillery, M. M. L., Zheng, C., Zheng, Y., & Megraw, T. L.
- Affiliations
- Florida State University (US)
- Xiamen University (CN)
- Corresponding author
- Megraw, Timothy L.
- Venue
- Molecular Biology of the Cell
- Published
- 2024-09-01
- Volume
- 35
- Issue
- 9
- Pages
- ar116-ar116
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC11449388
- OpenAlex
- W4400770621
- Cited by
- 9
- References
- 68
- Citations by year
- 2026: 3 · 2025: 6
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nc
- Locations
- Molecular Biology of the Cell (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Florida State University
- National Institutes of Health (IA 52242, NIH P40OD018537, P40OD018537)
- National Institute of General Medical Sciences (R01GM139971, R01GM068756)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- ninein, microtubule organization, MAP7, protein domain mapping, nuclear localization, centrosome, pericentriolar material, Seckel syndrome, fat body cells, Drosophila
- MeSH terms
- Protein Domains, Animals, Cell Nucleus, Centrioles, Drosophila, Drosophila melanogaster, Dyneins, Fat Body, Humans, Microtubule-Associated Proteins, Microtubules, Nuclear Proteins, Protein Binding, Centrosome, Homeodomain Proteins, Microtubule-Organizing Center, Drosophila Proteins
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- Tillery, M. M. L., Zheng, C., Zheng, Y., & Megraw, T. L. (2024). Ninein domains required for its localization, association with partners dynein and ensconsin, and microtubule organization. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e23-06-0245
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