Cited by 376Antioxidant Activity and Oxidative StressPhytochemicals and Antioxidant ActivitiesProteins in Food Systems

The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.

Carotenoid bioavailability is higher from salads ingested with full-fat than with fat-reduced salad dressings as measured with electrochemical detection

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Brown, Melody J, Ferruzzi, Mario G, Nguyen, Minhthy L, Cooper, Dale A, Eldridge, Alison L, Schwartz, Steven J, White, Wendy S·2004·The American Journal of Clinical Nutrition

Abstract

Background The amount of dietary fat required for optimal bioavailability of carotenoids in plant matrices is not clearly defined.Objective The objective was to quantify the appearance of carotenoids in plasma chylomicrons after subjects ingested fresh vegetable salads with fat-free, reduced-fat, or full-fat salad dressings.Design The subjects (n = 7) each consumed 3 salads consisting of equivalent amounts of spinach, romaine lettuce, cherry tomatoes, and carrots with salad dressings containing 0, 6, or 28 g canola oil. The salads were consumed in random order separated by washout periods of > or =2 wk. Blood samples were collected hourly from 0 to 12 h. Chylomicrons were isolated by ultracentrifugation, and carotenoid absorption was analyzed by HPLC with coulometric array detection.Results After ingestion of the salads with fat-free salad dressing, the appearance of alpha-carotene, beta-carotene, and lycopene in chylomicrons was negligible. After ingestion of the salads with reduced-fat salad dressing, the appearance of the carotenoids in plasma chylomicrons increased relative to that after ingestion of the salads with fat-free salad dressing (P < 0.04). Similarly, the appearance of the carotenoids in plasma chylomicrons was higher after the ingestion of salads with full-fat than with reduced-fat salad dressing (P < 0.02).Conclusions High-sensitivity HPLC with coulometric array detection enabled us to quantify the intestinal absorption of carotenoids ingested from a single vegetable salad. Essentially no absorption of carotenoids was observed when salads with fat-free salad dressing were consumed. A substantially greater absorption of carotenoids was observed when salads were consumed with full-fat than with reduced-fat salad dressing.

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Research works printed but not linked: 2

  1. 1996, Results from USDA's 1994-1996 Continuing Survey of Food Intakes by Individuals and 1994-96 Diet and Health Knowledge Survey, table set 10, table 4, 1994-96 Continuing Survey of Food Intakes by Individuals and 1994-96 Diet and Health Knowledge Survey.
  2. Alam, 1999, Effects of dietary fat supplementation during pregnancy/lactation on maternal blood and breastmilk vitamin A in rural Bangladesh, FASEB J, 13, A895

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Metadata

Authors
Brown, M. J., Ferruzzi, M. G., Nguyen, M. L., Cooper, D. A., Eldridge, A. L., Schwartz, S. J., & White, W. S.
Affiliations
Iowa State University (US)
Corresponding author
White, Wendy S
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
2004-08
Volume
80
Issue
2
Pages
396-403
ISSN
0002-9165 (ISSN-L)
OpenAlex
W2098537290
Cited by
376
References
48
Citations by year
2026: 7 · 2025: 11 · 2024: 11 · 2023: 15 · 2022: 20 · 2021: 27 · 2020: 17 · 2019: 27 · 2018: 14 · 2017: 24 · 2016: 18 · 2015: 21 · 2014: 18 · 2013: 24 · 2012: 24
FWCI
7.57
Type
journal article
Language
en
Open access
Locations
  • American Journal of Clinical Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
carotenoid bioavailability, salad dressing, dietary fat, chylomicrons, carotenoid absorption, lycopene, beta-carotene, alpha-carotene, canola oil, vegetable salad, plasma carotenoids
MeSH terms
Rapeseed Oil, Adult, Antioxidants, Biological Availability, Carotenoids, Chromatography, High Pressure Liquid, Chylomicrons, Fatty Acids, Monounsaturated, Female, Food Analysis, Humans, Intestinal Absorption, Male, Vegetables

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