CoChat
Sign Up
Cited by 87Muscle metabolism and nutritionFood and Agricultural SciencesAnimal Nutrition and Physiology

Ileal digestibility of intrinsically labeled hen's egg and meat protein determined with the dual stable isotope tracer method in Indian adults

Open access
Kashyap, Sindhu, Shivakumar, Nirupama, Varkey, Aneesia, Duraisamy, Rajendran, Thomas, Tinku, Preston, Thomas, Devi, Sarita, Kurpad, Anura V·2018·The American Journal of Clinical Nutrition
DOI 10.1093/ajcn/nqy178PMC PMC6250983
Open this paper in CoChat — free accountOpen PDF

Abstract

Background: Protein quality assessment through the Digestible Indispensable Amino Acid Score requires accurate measurements of true ileal protein and amino acid digestibility, for which a dual isotope technique was recently developed. However, the ileal digestibility of indispensable amino acids (IAA) in humans from high-quality proteins is not well known. Objective: The aim of this study was to intrinsically label hen's egg and meat protein by the use of uniformly 2H-labeled amino acids, and to measure their true ileal indispensable amino acid (IAA) digestibility via the dual isotope method in humans. Design: 2H-labeled lyophilized boiled egg white protein, whole boiled egg, and cooked meat were obtained from layer hens (BV-300) administered a uniformly 2H-labeled amino acid mix orally for 35 d with their daily feed. The ileal IAA digestibility of these proteins was determined with reference to digestibility of previously characterized [U-13C]spirulina in a dual tracer method in healthy Indian subjects whose intestinal health was measured by the plasma kynurenine-to-tryptophan (KT) ratio. Results: All subjects had normal KT ratios. The mean ± SD true ileal IAA digestibility of 2H-labeled egg white protein, whole boiled egg, and cooked meat was 86.3% ± 4.6%, 89.4% ± 4.5%, and 92.0% ± 2.8%, respectively. Leucine digestibility correlated with the KT ratio (r = -0.772; P = 0.009). Conclusions: Uniformly 2H-labeled hen's egg and meat protein can be used to measure ileal IAA digestibility by the dual isotope tracer approach in humans. The mean IAA digestibility values for these high-quality proteins in the healthy Indians studied were similar to values obtained in earlier human and animal experiments. Leucine digestibility in these meal matrices correlated with the KT ratio, but this aspect needs further evaluation. This trial was registered at the Clinical Trials Registry of India (http://ctri.nic.in) as CTRI/2018/03/012265.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1093/ajcn/nqy178

Cites

40 of 40 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The presence of nonprotein constituents and antinutritional factors are thought to slow and reduce the digestion and absorption of protein, meaning that a lower proportion of ingested amino acids become available at a reduced rate in the circulation postprandially.

    “The presence of nonprotein constituents and antinutritional factors are thought to slow (and reduce) the digestion and absorption of protein, meaning that a lower proportion of ingested amino acids become available (at a reduced rate) in the circulation postprandially [78,79].”

    From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [78]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Kashyap, S., Shivakumar, N., Varkey, A., Duraisamy, R., Thomas, T., Preston, T., Devi, S., & Kurpad, A. V.
Affiliations
St. John's National Academy of Health Sciences (IN)
Corresponding author
Kurpad, Anura V
ORCID
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
2018-11
Volume
108
Issue
5
Pages
980-987
ISSN
0002-9165 (ISSN-L)
DOI
10.1093/ajcn/nqy178
PMC
PMC6250983
OpenAlex
W2894780745
Cited by
87
References
40
Citations by year
2026: 6 · 2025: 15 · 2024: 11 · 2023: 8 · 2022: 10 · 2021: 12 · 2020: 12 · 2019: 11 · 2018: 2
FWCI
5.12
Type
journal article
Language
en
Open access
Licence
Locations
  • American Journal of Clinical Nutrition (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion, cc-by-sa)
Funders
Baylor College of Medicine
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
ileal protein digestibility, essential amino acids, meat proteins, kynurenine/tryptophan ratio, gut health, protein quality, Indian adults
MeSH terms
Meat Proteins, Adolescent, Adult, Amino Acids, Animals, Chickens, Cooking, Dietary Proteins, Digestion, Egg Proteins, Eggs, Female, Food Analysis, Humans, Ileum, India, Isotopes, Kynurenine, Male, Meat, Middle Aged, Tryptophan, Young Adult, Meals

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review