The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Improved Detection and Characterization of Paroxysmal Nocturnal Hemoglobinuria Using Fluorescent Aerolysin
Open accessAbstract
Paroxysmal nocturnal hemoglobinuria (PNH) is caused by a somatic mutation in the gene PIGA, which encodes an enzyme essential for the synthesis of glycosylphosphatidylinositol (GPI) anchors. The PIGA mutation results in absence or marked deficiency of more than a dozen proteins on PNH blood cells. Current flow cytometric assays for PNH rely on the use of labeled antibodies to detect deficiencies of specific GPI anchor proteins, such as CD59. However, because no single GPI anchor protein is always expressed in all cell lineages, no one monoclonal antibody can be used with confidence to diagnose PNH. We describe a new diagnostic test for PNH, based on the ability of a fluorescently labeled inactive variant of the protein aerolysin (FLAER) to bind selectively to GPI anchors. We compared GPI anchor protein expression in 8 patients with PNH using FLAER and anti-CD59. In all cases, FLAER detected similar or higher proportions of PNH monocytes and granulocytes compared with anti-CD59. Because of the increased sensitivity of detection, FLAER could detect small abnormal granulocyte populations in patients to a level of about 0.5%; samples from healthy control subjects contained substantially fewer FLAER-negative cells. FLAER gives a more accurate assessment of the GPI anchor deficit in PNH.
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Cites
Takeda, J., Miyata, T., Kawagoe, K., Iida, Y., Endo, Y., Fujita, T., Takahashi, M., Kitani, T., & Kinoshita, T. (1993) · Cell · doi:10.1016/0092-8674(93)90250-t · Cited by 1,029
Ravetch, J. V., & Perussia, B. (1989) · The Journal of experimental medicine · doi:10.1084/jem.170.2.481 · Cited by 569
The Cloning of PIG-A, a Component in the Early Step of GPI-Anchor Biosynthesis
Miyata, T., Takeda, J., Iida, Y., Yamada, N., Inoue, N., Takahashi, M., Maeda, K., Kitani, T., & Kinoshita, T. (1993) · Science · doi:10.1126/science.7680492 · Cited by 522
Paroxysmal nocturnal haemoglobinuria (PNH) is caused by somatic mutations in the PIG-A gene.
Bessler, M., Mason, P. J., Hillmen, P., Miyata, T., Yamada, N., Takeda, J., Luzzatto, L., & Kinoshita, T. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06240.x · Cited by 389
Yamashina, M., Ueda, E., Kinoshita, T., Takami, T., Ojima, A., Ono, H., Tanaka, H., Kondo, N., Orii, T., Okada, N., et al. (1990) · New England Journal of Medicine · doi:10.1056/nejm199010253231707 · Cited by 351
Miyata, T., Yamada, N., Iida, Y., Nishimura, J., Takeda, J., Kitani, T., & Kinoshita, T. (1994) · New England Journal of Medicine · doi:10.1056/nejm199401273300404 · Cited by 236
7 Paroxysmal nocturnal haemoglobinuria
Rotoli, B., & Luzzatto, L. (1989) · Baillière's Clinical Haematology · doi:10.1016/s0950-3536(89)80010-1 · Cited by 231
Hall, S., & Rosse, W. (1996) · Blood · doi:10.1182/blood.v87.12.5332.bloodjournal87125332 · Cited by 220
Anchoring mechanisms for LFA-3 cell adhesion glycoprotein at membrane surface
Dustin, M. L., Selvaraj, P., Mattaliano, R. J., & Springer, T. A. (1987) · Nature · doi:10.1038/329846a0 · Cited by 217
van der Schoot, C., Huizinga, T., van 't Veer-Korthof, E., Wijmans, R., Pinkster, J., & von dem Borne, A. (1990) · Blood · doi:10.1182/blood.v76.9.1853.1853 · Cited by 195
Cited by
Paroxysmal nocturnal hemoglobinuria
Brodsky, R. A. (2014) · Blood · doi:10.1182/blood-2014-02-522128 · Cited by 648
Self-citationTkachenko, E., Lutgens, E., Stan, R.-V., & Simons, M. (2004) · Journal of Cell Science · doi:10.1242/jcs.01190 · Cited by 141
Metadata
- Authors
- Brodsky, R. A., Mukhina, G. L., Li, S., Nelson, K. L., Chiurazzi, P. L., Buckley, J. T., & Borowitz, M. J.
- Venue
- American Journal of Clinical Pathology
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-09-01
- Volume
- 114
- Issue
- 3
- Pages
- 459-466
- ISSN
- 0002-9173 (ISSN-L)
- 1943-7722
- OpenAlex
- W1952266054
- Cited by
- 271
- References
- 20
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Brodsky, R. A., Mukhina, G. L., Li, S., Nelson, K. L., Chiurazzi, P. L., Buckley, J. T., & Borowitz, M. J. (2000). Improved Detection and Characterization of Paroxysmal Nocturnal Hemoglobinuria Using Fluorescent Aerolysin. American Journal of Clinical Pathology. https://doi.org/10.1093/ajcp/114.3.459
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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