The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo
Abstract
Sulforaphane (SFN), a constituent of cruciferous vegetables, is highly effective in affording protection against chemically induced cancers in animal models. Here, we report that SFN inhibited proliferation of cultured PC-3 human prostate cancer cells by inducing apoptosis that was characterized by appearance of cells with sub-G0/G1 DNA content, formation of cytoplasmic histone associated DNA fragments and cleavage of poly(ADP-ribose)polymerase (PARP). SFN-induced apoptosis was associated with up-regulation of Bax, down-regulation of Bcl-2 and activation of caspases-3, -9 and -8. SFN-induced apoptosis, and cleavage of procaspase-3 and PARP were blocked upon pre-treatment of cells with pan caspase inhibitor z-VADfmk, and specific inhibitors of caspase-9 (z-LEHDfmk) and caspase-8 (z-IETDfmk) suggesting involvement of both caspase-9 and caspase-8 pathways in SFN-induced cell death. Oral administration of SFN (5.6 micro mol, 3 times/week) significantly inhibited growth of PC-3 xenografts in nude mice. For instance, 10 days after starting therapy, the average tumor volumes in control and SFN-treated mice were 170 +/- 13 and 80 +/- 14 mm3, respectively, reflecting a >50% reduction in tumor volume due to SFN administration. To the best of our knowledge, the present study is the first published report to document in vivo anticancer activity of SFN in a tumor xenograft model.
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Activation of Nrf2-antioxidant signaling attenuates NFκB-inflammatory response and elicits apoptosis
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Bioavailability of Glucosinolates and Their Breakdown Products: Impact of Processing
Barba, F. J., Nikmaram, N., Roohinejad, S., Khelfa, A., Zhu, Z., & Koubaa, M. (2016) · Frontiers in Nutrition · doi:10.3389/fnut.2016.00024 · Cited by 336
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Cancer-preventive isothiocyanates: measurement of human exposure and mechanism of action
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Metadata
- Authors
- Singh, A. V.
- Venue
- Carcinogenesis
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-09-26
- Volume
- 25
- Issue
- 1
- Pages
- 83-90
- ISSN
- 0143-3334 (ISSN-L)
- 1460-2180
- OpenAlex
- W2118287650
- Cited by
- 357
- References
- 43
- FWCI
- 5.36
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. https://app.cochat.ai/papers/10.1093/carcin/bgg178
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- Singh, A. V. (2003). Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. Carcinogenesis. https://doi.org/10.1093/carcin/bgg178
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