The DOI matches its Crossref record; 65 of the 71 references it cites were found in a registry or an index.
Tissue-resident stem cells promote breast cancer growth and metastasis
Open accessAbstract
Mesenchymal stem cells derived from bone marrow have recently been described to localize to breast carcinomas and to integrate into the tumor-associated stroma. In the present study, we investigated whether adipose tissue-derived stem cells (ASCs) could play a role in tumor growth and invasion. Compared with bone marrow-derived cells, ASCs as tissue-resident stem cells are locally adjacent to breast cancer cells and may interact with tumor cells directly. Here, we demonstrate that ASCs cause the cancer to grow significantly faster when added to a murine breast cancer 4T1 cell line. We further show that breast cancer cells enhance the secretion of stromal cell-derived factor-1 from ASCs, which then acts in a paracrine fashion on the cancer cells to enhance their motility, invasion and metastasis. The tumor-promoting effect of ASCs was abolished by knockdown of the chemokine C-X-C receptor 4 in 4T1 tumor cells. We demonstrated that ASCs home to tumor site and promote tumor growth not only when co-injected locally but also when injected intravenously. Furthermore, we demonstrated that ASCs incorporate into tumor vessels and differentiate into endothelial cells. The tumor-promoting effect of tissue-resident stem cells was also tested and validated using a human breast cancer line MDA-MB-231 cells and human adipose tissue-derived stem cells. Our findings indicate that the interaction of local tissue-resident stem cells with tumor stem cells plays an important role in tumor growth and metastasis.
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“Another MSC-secreted pro-metastasis cytokine, CCL5 (RANTES), can be secreted upon interaction with cancer cells and is associated with tumor progression and invasion in various cancers [73, 87, 100, 142–144].”
From Mesenchymal stem cell secretome and regenerative therapy after cancer (2013) · cites this paper as [73]
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Cites
Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies
Zuk, P. A., Zhu, M., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., Benhaim, P., Lorenz, H. P., & Hedrick, M. H. (2001) · Tissue Engineering · doi:10.1089/107632701300062859 · Cited by 7,956
Human Adipose Tissue Is a Source of Multipotent Stem Cells
Zuk, P. A., Zhu, M., Ashjian, P. H., Ugarte, D. A. D., Huang, J. I., Mizuno, H., Alfonso, Z. C., Fraser, J. K., Benhaim, P., & Hedrick, M. H. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-02-0105 · Cited by 6,762
Orimo, A., Gupta, P. B., Sgroi, D. C., Arenzana-Seisdedos, F., Delaunay, T., Naeem, R., Carey, V. J., Richardson, A. L., & Weinberg, R. A. (2005) · Cell · doi:10.1016/j.cell.2005.02.034 · Cited by 3,759
Mesenchymal stem cells within tumour stroma promote breast cancer metastasis
Karnoub, A. E., Dash, A. B., Vo, A. P., Sullivan, A., Brooks, M. W., Bell, G. W., Richardson, A. L., Polyak, K., Tubo, R., & Weinberg, R. A. (2007) · Nature · doi:10.1038/nature06188 · Cited by 3,206
In vitro propagation and transcriptional profiling of human mammary stem/progenitor cellsPreprint
Dontu, G., Abdallah, W. M., Foley, J. M., Jackson, K. W., Clarke, M. F., Kawamura, M., & Wicha, M. S. (2003) · Genes & Development · doi:10.1101/gad.1061803 · Cited by 2,413
Stromal fibroblasts in cancer initiation and progression
Bhowmick, N. A., Neilson, E. G., & Moses, H. L. (2004) · Nature · doi:10.1038/nature03096 · Cited by 2,354
Cancer and the chemokine network
Balkwill, F. (2004) · Nature Reviews Cancer · doi:10.1038/nrc1388 · Cited by 2,343
Ponti, D., Costa, A., Zaffaroni, N., Pratesi, G., Petrangolini, G., Coradini, D., Pilotti, S., Pierotti, M. A., & Daidone, M. G. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-0626 · Cited by 1,725
Carcinoma-associated fibroblasts stimulate tumor progression of initiated human epithelium
Olumi, A., Grossfeld, G., Hayward, S., Carroll, P., Cunha, G., Hein, P., & Tlsty, T. (2000) · Breast Cancer Research · doi:10.1186/bcr138 · Cited by 1,463
Role of tissue stroma in cancer cell invasion
De Wever, O., & Mareel, M. (2003) · The Journal of Pathology · doi:10.1002/path.1398 · Cited by 1,042
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6 of the 71 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- Hsieh, 1999, Stem cell differentiation and reduction as a potential mechanism for chemoprevention of breast cancer, Chinese Pharm. J., 51, 15
- Chang, 2000, Roles of ionizing radiation in neoplastic transformation of human breast epithelial cells, 576
Cited by
Sorted by citations
Items 301–329 of 329
Mesenchymal Stem/Stromal Cell Recruitment by Central Nervous System Tumors
Kerrigan, B. C. P., & Lang, F. F. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-803102-5.00010-0
Mesenchymal Stem Cells in Laryngopharyngeal Cancer
Park, Y., Lee, B., & Shin, S. (2014) · Journal of Clinical Otolaryngology Head and Neck Surgery · doi:10.35420/jcohns.2014.25.2.137
Trivedi, M. K., Branton, A., Trivedi, D., Nayak, G., Mondal, S. C., & Jana, S. (2018) · Trends journal of sciences research · doi:10.31586/biochemistry.0303.04
Importancia de la investigación sobre el estroma tumoral en el cáncer de mama
Eiró, N., Cid, S., Fraile, M., & Vizoso, F. J. (2019) · Revista de Senología y Patología Mamaria · doi:10.1016/j.senol.2019.01.001
Ulpiano, C., Alves, C. P. A., Silva-Santos, A. R., Serra, J., da Silva, C. L., & Monteiro, G. A. (2019) · IEEE Portuguese Meeting on Bioengineering · doi:10.1109/enbeng.2019.8692558
Early or Late Recurrences of Breast Carcinoma Are to Be Researched in Relation to Fat Grafting
Riggio, E. (2019) · Surgical Techniques Development · doi:10.4081/std.2019.8067
Our 3-year Experience of Stem Cell Use in Treatment of Deep Skin Burns
Zinovye, E. (2019) · Biomedical Journal of Scientific & Technical Research · doi:10.26717/bjstr.2019.16.002872
The Age-Dependent Characterization of the ER-Alpha Positive Breast Cancer Tumor Microenvironment
Hamel, K. M. (2021) · Louisiana State University · doi:10.31390/gradschool_dissertations.5482
Morrison, W. A. (2021) · doi:10.1093/med/9780199682874.003.0094
Aoki, M., Kakimoto, K., Goto, M., & Higuchi, K. (2021) · Scientific Reports · doi:10.1038/s41598-021-99148-5
RetractedProstate tumor cell growth induced by proliferin secreted from castrated mice adipose stromal cells
Hirayama, T., Matsumoto, K., & Minami, S. (2014) · Kitasato medical journal
Novel compounds inhibiting HIV infection, breast cancer metastasis, and bacterial growth
Shepard, J. B. (2012) · Montana State University ScholarWorks (Montana State University)
Guha, S., Ramachandran, V., & Arumugam, T. (2009) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.1165012.625889
6 Fat Grafting for Primary Augmentation
(2020) · Thieme eBooks · doi:10.1055/b-0040-177583
Shiffman, M. A. (2014) · Springer eBooks · doi:10.1007/978-3-642-45207-9_56
Lim, S. K., Yian, S. S., Ismael, L. Q., Bee, Y. T. G., Harn, G. L., & Yin, K. B. (2024) · Molecular Biology Reports · doi:10.1007/s11033-024-09269-z
Kang, H. S., Kang, M. J., Hong, H. K., Ryu, J. Y., Lee, J. S., Choi, K. Y., Chung, H. Y., Park, H. Y., & Yang, J. D. (2025) · Journal of Wound Management and Research · doi:10.22467/jwmr.2024.02964
Eiró, N., Fraile, M., Escudero-Cernuda, S., Sendón-Lago, J., Gonzalez, L. O., Fernández‐Sánchez, M. L., & Vizoso, F. J. (2024) · Figshare · doi:10.6084/m9.figshare.25698412
Eiró, N., Fraile, M., Escudero-Cernuda, S., Sendón-Lago, J., Gonzalez, L. O., Fernández‐Sánchez, M. L., & Vizoso, F. J. (2024) · Figshare · doi:10.6084/m9.figshare.25698279
Sagini, M. N., Berger, M. R., Muthmann, J., Alt, E., & Forssmann, W. (2026) · Peptides · doi:10.1016/j.peptides.2026.171479
Self-citationLong-Term Outcomes of Patients Having Breast-Conserving Surgery and Immediate Lipofilling
Dixon, J. M., Swan, R., Black, S., Klenjnotowska, A., & Lucocq, J. E. (2026) · Journal of Plastic and Reconstructive Surgery · doi:10.53045/jprs.2026-0039
The role of the chemokine receptor CXCR4 in EGFRvIII-expressing breast cancer
Rahimi, M. (2009) · DigitalGeorgetown (Georgetown University Library)
Interactions Between Adipose Stem Cells and Cancer
Chung, C., & Rubin, J. P. (2014) · Springer eBooks · doi:10.1007/978-3-642-45207-9_55
Complications of Stem Cell-Assisted Fat Transfer
Shiffman, M. A. (2014) · Springer eBooks · doi:10.1007/978-3-642-45207-9_54
Metadata
- Authors
- Muehlberg, F. L., Song, Y.-H., Krohn, A., Pinilla, S. P., Droll, L. H., Leng, X., Seidensticker, M., Ricke, J., Altman, A. M., Devarajan, E., Liu, W., Arlinghaus, R. B., & Alt, E. U.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- University Hospital Magdeburg (DE)
- Corresponding author
- Alt, Eckhard U.
- ORCID
- Venue
- Carcinogenesis
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-01-30
- Volume
- 30
- Issue
- 4
- Pages
- 589-597
- ISSN
- 0143-3334 (ISSN-L)
- 1460-2180
- OpenAlex
- W2107402669
- Cited by
- 330
- References
- 71
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 13 · 2023: 9 · 2022: 11 · 2021: 19 · 2020: 10 · 2019: 17 · 2018: 18 · 2017: 13 · 2016: 20 · 2015: 33 · 2014: 35 · 2013: 24 · 2012: 31
- FWCI
- 16.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Carcinogenesis (publishedVersion)
- PubMed (publishedVersion)
- BORIS (University Library Bern) (submittedVersion, other-oa)
- Open Access CRIS of the University of Bern
- Funders
- U.S. Department of Defense (W81XWH, W81XWH0810523)
- Topics
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- tissue-resident stem cells, adipose-derived stem cells, breast cancer, tumor growth, metastasis, CXCL12, CXCR4, paracrine signaling, tumor cell motility, tumor invasion, stem cell homing, tumor vasculature, endothelial differentiation, tumor microenvironment, 4T1 cells, MDA-MB-231 cells
- MeSH terms
- Adipose Tissue, Animals, Cell Movement, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Lung Neoplasms, Male, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Neovascularization, Pathologic, Neoplastic Stem Cells, Blotting, Western, Mammary Neoplasms, Animal, Stromal Cells, Spheroids, Cellular, Receptors, CXCR4, Vascular Endothelial Growth Factor A, Immunoprecipitation, Mice, Chemokine CXCL12
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