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MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL

Baraniskin, Alexander, Birkenkamp-Demtroder, Karin, Maghnouj, Abdelouahid, Zöllner, Hannah, Munding, Johanna, Klein-Scory, Susanne, Reinacher-Schick, Anke, Schwarte-Waldhoff, Irmgard, Schmiegel, Wolff, Hahn, Stephan A.·2012·Carcinogenesis
DOI 10.1093/carcin/bgs020
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that are involved in different biological processes by suppressing target gene expression. Altered expression of miR-30a-5p has been reported in colon carcinoma. To elucidate its potential biological role in colon cancer, miR-30a-5p was overexpressed via a lentiviral vector system in two different colon cancer cell lines. This induced in both lines miR-30a-5p-mediated growth inhibition, attributable to a cell cycle arrest at the G(1) phase and an induction of apoptosis. Combining global gene expression analyses of miR-30a-5p transgenic line HCT116 with in silico miRNA target prediction, we identified the denticleless protein homolog (DTL) as a potential miRNA-30a-5p target. Subsequent reporter gene assays confirmed the predicted miR-30a-5p binding site in the 3'untranslated region of DTL. Importantly, overexpression of DTL in HCT116 cells partially rescued these cells from miR-30a-5p-mediated growth suppression. In addition, TP53 and CDKN1A expression were increased in miR-30a-5p-overexpressing HCT116 cells, suggesting that miR-30a-5p is able to modulate the cell cycle via a DTL-TP53-CDKN1A regulatory circuit. Finally, 379 colorectal cancer tissues were screened for DTL expression and DTL was found to be overexpressed in 95.8% of human colorectal cancers compared with normal colon mucosa. In conclusion, our data identified miR-30a-5p as a tumor-suppressing miRNA in colon cancer cells exerting its function via modulation of DTL expr…

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Research works printed but not linked: 38

  1. Bartel, 2004, MicroRNAs: genomics, biogenesis, mechanism, and function, Cell, 116, 281, 10.1016/S0092-8674(04)00045-5 · DOI
  2. Ventura, 2009, MicroRNAs and cancer: short RNAs go a long way, Cell, 136, 586, 10.1016/j.cell.2009.02.005 · DOI
  3. Parkin, 2002, Global cancer statistics, CA Cancer J. Clin., 55, 74, 10.3322/canjclin.55.2.74 · DOI
  4. Luo, 2011, MicroRNA signatures: novel biomarker for colorectal cancer?, Cancer Epidemiol. Biomarkers Prev., 20, 1272, 10.1158/1055-9965.EPI-11-0035 · DOI
  5. Cummins, 2006, The colorectal microRNAome, Proc. Natl Acad. Sci. USA, 103, 3687, 10.1073/pnas.0511155103 · DOI · Paper page
  6. Arndt, 2009, Characterization of global microRNA expression reveals oncogenic potential of miR-145 in metastatic colorectal cancer, BMC Cancer, 9, 374, 10.1186/1471-2407-9-374 · DOI
  7. Sarver, 2009, Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states, BMC Cancer, 9, 401, 10.1186/1471-2407-9-401 · DOI
  8. Visone, 2007, Specific microRNAs are downregulated in human thyroid anaplastic carcinomas, Oncogene, 26, 7590, 10.1038/sj.onc.1210564 · DOI
  9. Kumarswamy, 2011, MicroRNA-30a inhibits epithelial-to-mesenchymal transition by targeting snai1 and is downregulated in non-small cell lung cancer, Int. J. Cancer, 130, 2044, 10.1002/ijc.26218 · DOI
  10. Calin, 2004, MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias, Proc. Natl Acad. Sci. USA, 101, 11755, 10.1073/pnas.0404432101 · DOI
  11. Li, 2010, Survival prediction of gastric cancer by a seven-microRNA signature, Gut, 59, 579, 10.1136/gut.2008.175497 · DOI
  12. Martinez, 2011, miR-29 and miR-30 regulate B-Myb expression during cellular senescence, Proc. Natl Acad. Sci. USA, 108, 522, 10.1073/pnas.1017346108 · DOI
  13. Kimura, 2010, Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC, Oncol. Rep., 23, 1625
  14. Andersen, 2009, Dysregulation of the transcription factors SOX4, CBFB and SMARCC1 correlates with outcome of colorectal cancer, Br. J. Cancer, 100, 511, 10.1038/sj.bjc.6604884 · DOI
  15. Birkenkamp-Demtroder, 2007, Phosphoprotein Keratin 23 accumulates in MSS but not MSI colon cancers in vivo and impacts viability and proliferation in vitro, Mol. Oncol., 1, 181, 10.1016/j.molonc.2007.05.005 · DOI
  16. Munding, 2011, Global microRNA expression profiling of microdissected tissues identifies miR-135b as a novel biomarker for pancreatic ductal adenocarcinoma, Int. J. Cancer
  17. Gentner, 2009, Stable knockdown of microRNA in vivo by lentiviral vectors, Nat. Methods, 6, 63, 10.1038/nmeth.1277 · DOI
  18. Abbas, 2008, PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex, Genes Dev., 22, 2496, 10.1101/gad.1676108 · DOI
  19. Solly, 2004, Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays, Assay Drug Dev. Technol., 2, 363, 10.1089/adt.2004.2.363 · DOI
  20. Kirstein, 2006, Live cell quality control and utility of real-time cell electronic sensing for assay development, Assay Drug Dev. Technol., 4, 545, 10.1089/adt.2006.4.545 · DOI
  21. Chomczynski, 1987, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem., 162, 156, 10.1016/0003-2697(87)90021-2 · DOI
  22. Wei, 2009, Both strands of siRNA have potential to guide posttranscriptional gene silencing in mammalian cells, PLoS One, 4, e5382, 10.1371/journal.pone.0005382 · DOI
  23. Cheung, 2001, Cloning and expression of a novel nuclear matrix-associated protein that is regulated during the retinoic acid-induced neuronal differentiation, J. Biol. Chem., 276, 17083, 10.1074/jbc.M010802200 · DOI
  24. Li, 2009, Identification of retinoic acid-regulated nuclear matrix-associated protein as a novel regulator of gastric cancer, Br. J. Cancer, 101, 691, 10.1038/sj.bjc.6605202 · DOI
  25. Missiaglia, 2009, Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: an approach to identify candidate genes involved in tumor development, Genes Chromosomes Cancer, 48, 455, 10.1002/gcc.20655 · DOI
  26. Pan, 2006, Role of L2DTL, cell cycle-regulated nuclear and centrosome protein, in aggressive hepatocellular carcinoma, Cell Cycle, 5, 2676, 10.4161/cc.5.22.3500 · DOI
  27. Song, 2010, Molecular mechanism of chemoresistance by miR-215 in osteosarcoma and colon cancer cells, Mol. Cancer, 9, 96, 10.1186/1476-4598-9-96 · DOI
  28. Ueki, 2008, Involvement of elevated expression of multiple cell-cycle regulator, DTL/RAMP (denticleless/RA-regulated nuclear matrix associated protein), in the growth of breast cancer cells, Oncogene, 27, 5672, 10.1038/onc.2008.186 · DOI
  29. Timofeev, 2004, Functions of p21Waf1 in norm and in stress, Mol. Biol. (Mosk.), 38, 371, 10.1023/B:MBIL.0000032199.57820.43 · DOI
  30. Nishitani, 2008, CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation, J. Biol. Chem., 283, 29045, 10.1074/jbc.M806045200 · DOI
  31. Banks, 2006, L2DTL/CDT2 and PCNA interact with p53 and regulate p53 polyubiquitination and protein stability through MDM2 and CUL4A/DDB1 complexes, Cell Cycle, 5, 1719, 10.4161/cc.5.15.3150 · DOI · Paper page
  32. Xi, 2006, Prognostic values of microRNAs in colorectal cancer, Biomark. Insights, 2, 113
  33. Lim, 2005, Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs, Nature, 433, 769, 10.1038/nature03315 · DOI · Paper page
  34. Grimson, 2007, MicroRNA targeting specificity in mammals: determinants beyond seed pairing, Mol. Cell, 27, 91, 10.1016/j.molcel.2007.06.017 · DOI · Paper page
  35. Taylor, 2001, Regulation of the G2/M transition by p53, Oncogene, 20, 1803, 10.1038/sj.onc.1204252 · DOI
  36. Enserink, 2010, An overview of Cdk1-controlled targets and processes, Cell Div., 5, 11, 10.1186/1747-1028-5-11 · DOI
  37. Subramanyam, 2011, Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells, Nat. Biotechnol., 29, 443, 10.1038/nbt.1862 · DOI
  38. Unsal-Kaçmaz, 2005, Coupling of human circadian and cell cycles by the timeless protein, Mol. Cell. Biol., 25, 3109, 10.1128/MCB.25.8.3109-3116.2005 · DOI · Paper page

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Metadata

Authors
Baraniskin, A., Birkenkamp-Demtroder, K., Maghnouj, A., Zöllner, H., Munding, J., Klein-Scory, S., Reinacher-Schick, A., Schwarte-Waldhoff, I., Schmiegel, W., & Hahn, S. A.
Venue
Carcinogenesis
Publisher
Oxford University Press (OUP)
Published
2012-01-27
Volume
33
Issue
4
Pages
732-739
ISSN
  • 1460-2180
  • 0143-3334
DOI
10.1093/carcin/bgs020
Cited by
142
References
4
Language
en

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