The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients
Open accessAbstract
Background: Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription–polymerase chain reaction (RT-PCR) method has limitations for clinical diagnosis and treatment. Methods: A total of 323 samples from 76 COVID-19–confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based 2 target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging. Results: In 95 samples that tested positive by both methods, the cycle threshold (Ct) of RT-PCR was highly correlated with the copy number of ddPCR (ORF1ab gene, R2 = 0.83; N gene, R2 = 0.87). Four (4/161) negative and 41 (41/67) single-gene positive samples tested by RT-PCR were positive according to ddPCR with viral loads ranging from 11.1 to 123.2 copies/test. The viral load of respiratory samples was then compared and the average viral load in sputum (17 429 ± 6920 copies/test) was found to be significantly higher than in throat swabs (2552 ± 1965 copies/test, P < .001) and nasal swabs (651 ± 501 copies/test, P < .001). Furthermore, the viral loads in the early and progressive stages were significantly higher than that in the recovery stage (46 800 ± 17 272 vs 1252 ± 1027, P < .001) analyzed by sputum samples. Conclusions: Quantitative monitoring of viral load in lower respiratory tract samples helps to evaluate disease progression, especially in cases of low viral load.
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Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia
Li, Q., Guan, X., Wu, P., Wang, X., Zhou, L., Tong, Y., Ren, R., Leung, K. S. M., Lau, E. H. Y., Wong, J. Y., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2001316 · Cited by 16,575
Self-citationA new coronavirus associated with human respiratory disease in China
Wu, F., Zhao, S., Yu, B., Chen, Y.-M., Wang, W., Song, Z.-G., Hu, Y., Tao, Z.-W., Tian, J.-H., Pei, Y.-Y., et al. (2020) · Nature · doi:10.1038/s41586-020-2008-3 · Cited by 11,688
First case of 2019 novel coronavirus in the United States
Holshue, M. L., DeBolt, C., Lindquist, S., Lofy, K. H., Wiesman, J., Bruce, H., Spitters, C., Ericson, K., Wilkerson, S., Tural, A., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2001191 · Cited by 6,030
Viral load of SARS-CoV-2 in clinical samples.
Pan, Y., Zhang, D., Yang, P., Poon, L. L. M., & Wang, Q. (2020) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(20)30113-4 · Cited by 1,562
Accurate Quantification and Characterization of Adeno-Associated Viral Vectors
Dobnik, D., Kogovšek, P., Jakomin, T., Košir, N., Tušek Žnidarič, M., Leskovec, M., Kaminsky, S. M., Mostrom, J., Lee, H., & Ravnikar, M. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01570 · Cited by 123
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Coronavirus Disease 2019–COVID-19
Dhama, K., Khan, S., Tiwari, R., Sircar, S., Bhat, S., Malik, Y. S., Singh, K. P., Chaicumpa, W., Bonilla-Aldana, D. K., & Rodriguez-Morales, A. J. (2020) · Clinical Microbiology Reviews · doi:10.1128/cmr.00028-20 · Cited by 1,876
SARS-CoV-2 detection, viral load and infectivity over the course of an infection
Walsh, K. A., Jordan, K., Clyne, B., Rohde, D., Drummond, L., Byrne, P., Ahern, S., Carty, P. G., O'Brien, K. K., O'Murchu, E., et al. (2020) · Journal of Infection · doi:10.1016/j.jinf.2020.06.067 · Cited by 608
A Systematic Review of the Clinical Utility of Cycle Threshold Values in the Context of COVID-19
Rao, S. N., Manissero, D., Steele, V. R., & Pareja, J. (2020) · Infectious Diseases and Therapy · doi:10.1007/s40121-020-00324-3 · Cited by 398
COVID-19 diagnostics in context
Weissleder, R., Lee, H., Ko, J., & Pittet, M. J. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.abc1931 · Cited by 396
Molecular Diagnosis of COVID-19: Challenges and Research Needs
Feng, W., Newbigging, A. M., Le, C., Pang, B., Peng, H., Cao, Y., Wu, J., Abbas, G., Song, J., Wang, D.-B., et al. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c02060 · Cited by 389
Metadata
- Authors
- Yu, F., Yan, L., Wang, N., Yang, S., Wang, L., Tang, Y., Gao, G., Wang, S., Ma, C., Xie, R., Wang, F., Tan, C., Zhu, L., Guo, Y., & Zhang, F.
- Venue
- Clinical Infectious Diseases
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-03-28
- Volume
- 71
- Issue
- 15
- Pages
- 793-798
- ISSN
- 1058-4838 (ISSN-L)
- 1537-6591
- OpenAlex
- W3013776441
- Cited by
- 680
- References
- 5
- FWCI
- 18.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yu, F., Yan, L., Wang, N., Yang, S., Wang, L., Tang, Y., Gao, G., Wang, S., Ma, C., Xie, R., Wang, F., Tan, C., Zhu, L., Guo, Y., & Zhang, F. (2020). Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients. Clinical Infectious Diseases. https://doi.org/10.1093/cid/ciaa345
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