The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage
Abstract
A simple, reliable, and reproducible fluorometric method for measuring thiobarbituric acid-reactive substances (TBARS) in serum is proposed, based on the reaction between malondialdehyde (MDA) and thiobarbituric acid. Formation of TBARS was complete at pH 2.4-2.6, but extraction with n-butanol proved complete only at lower pH, i.e., 1.6-1.7. Analytical recoveries of MDA added to serum were 94%-101%; within- and between-run CVs were 2.4-3.6% and 4.6-5.5%; and the detection limit for TBARS in serum was 0.10 mumol/L. Optimized conditions included: (a) collection of either serum or heparinized plasma, (b) preservation from in vitro autoxidation by glutathione and EDTA, and (c) storage at -20 degrees C up to 35 days. The mean (+/- SD) TBARS concentration in 47 healthy adults was 1.01 (0.21) mumol/L; no sex-related difference was observed. Higher concentrations were measured in patients with renal insufficiency undergoing hemodialysis and in patients with insulin-dependent diabetes, chronic pancreatitis, or liver cirrhosis.
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The measurement and mechanism of lipid peroxidation in biological systems
Gutteridge, J. M. C., & Halliwell, B. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90206-q · Cited by 1,283
Ledwoż;yw, A., Michalak, J., Stȩpień, A., & Ka̧dziołka, A. (1986) · Clinica Chimica Acta · doi:10.1016/0009-8981(86)90247-0 · Cited by 520
Pryor, W. A., Stanley, J. P., & Blair, E. (1976) · Lipids · doi:10.1007/bf02532843 · Cited by 503
Richard, M. J., Portal, B., Meo, J., Coudray, C., Hadjian, A., & Favier, A. (1992) · Clinical Chemistry · doi:10.1093/clinchem/38.5.704 · Cited by 304
Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation.
Knight, J. A., Pieper, R. K., & McClellan, L. (1988) · Clinical Chemistry · doi:10.1093/clinchem/34.12.2433 · Cited by 242
Evidence for membrane lipid peroxidation during the in vivo aging of human erythrocytes
Jain, S. K. (1988) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(88)90242-8 · Cited by 211
Gutteridge, J. M. C. (1982) · International Journal of Biochemistry · doi:10.1016/0020-711x(82)90050-7 · Cited by 191
Lepage, G., Munoz, G., Champagne, J., & Roy, C. C. (1991) · Analytical Biochemistry · doi:10.1016/0003-2697(91)90392-7 · Cited by 178
Free and bound malondialdehyde measured as thiobarbituric acid adduct by HPLC in serum and plasma
Carbonneau, M. A., Peuchant, E., Sess, D., Canioni, P., & Clerc, M. (1991) · Clinical Chemistry · doi:10.1093/clinchem/37.8.1423 · Cited by 145
Richard, M., Guiraud, P., Meo, J., & Favier, A. E. (1992) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/0378-4347(92)80593-f · Cited by 139
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Del Rio, D., Stewart, A. J., & Pellegrini, N. (2005) · Nutrition, Metabolism and Cardiovascular Diseases · doi:10.1016/j.numecd.2005.05.003 · Cited by 2,721
Tsikas, D. (2017) · Analytical Biochemistry · doi:10.1016/j.ab.2016.10.021 · Cited by 2,190
Improved analysis of malondialdehyde in human body fluids
Jentzsch, A. M., Bachmann, H., Fürst, P., & Biesalski, H. K. (1996) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)02043-8 · Cited by 802
Martín-Gallán, P., Carrascosa, A., Gussinyé, M., & Domínguez, C. (2003) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(03)00185-0 · Cited by 398
Antioxidant enzyme activities and oxidative stress in affective disorders
Erkan Ozcan, M., Gulec, M., Ozerol, E., Polat, R., & Akyol, O. (2004) · International Clinical Psychopharmacology · doi:10.1097/00004850-200403000-00006 · Cited by 368
Metadata
- Authors
- Wasowicz, W., Nève, J., & Peretz, A.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1993-12-01
- Volume
- 39
- Issue
- 12
- Pages
- 2522-2526
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2148182857
- Cited by
- 639
- References
- 16
- FWCI
- 1.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wasowicz, W., Nève, J., & Peretz, A. (1993). Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clinical Chemistry. https://doi.org/10.1093/clinchem/39.12.2522
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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