The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Blood and plasma glutathione measured in healthy subjects by HPLC: relation to sex, aging, biological variables, and life habits
Abstract
We report an HPLC method for measuring the concentrations of reduced (GSH) and total (GSHt) free glutathione in human plasma and whole blood. The chromatographic step was coupled with a postcolumn derivatization reaction and fluorometric detection. The linear range was 0.81-13.02 mumol/L, and the detection limit was 0.13 mumol/L. In healthy adults (ages 18-73 years), mean concentrations were 941 +/- 155 mumol/L for GSHt and 849 +/- 63 mumol/L for GSH in blood (107 men, 94 women), and 3.39 +/- 1.04 mumol/L for GSH in plasma (66 men, 58 women). Blood GSHt but not GSH was significantly lower in children (32 boys, 32 girls: 872 +/- 157 mumol/L) than in adults. Blood GSHt and GSH appeared to be correlated positively with the number of cigarettes smoked per day and the regular practice of physical exercise, and negatively with alcohol abstinence. We observed positive correlations between blood GSHt and cholesterol and calcium concentrations, and between blood GSH and cholesterol concentration.
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Cites
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Selective Modification of Glutathione Metabolism
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“The tripeptide glutathione (gamma-L-glutamyl-Lcysteinylglycine) is the major intracellular nonprotein thiol compound and plays a major role in the protection of cells and tissue structures (1).”
BackgroundSYSTEMIC GLUTATHIONE DEFICIENCY IN SYMPTOM-FREE HIV-SEROPOSITIVE INDIVIDUALS
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Partners in defense, vitamin E and vitamin C
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Glutathione deficiency in alcoholics: risk factor for paracetamol hepatotoxicity.
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Blood glutathione oxidation during human exercise
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Cited by
ZITKA, O., SKALICKOVA, S., GUMULEC, J., MASARIK, M., ADAM, V., HUBALEK, J., TRNKOVA, L., KRUSEOVA, J., ECKSCHLAGER, T., & KIZEK, R. (2012) · Oncology Letters · doi:10.3892/ol.2012.931 · Cited by 664
Glutathione measurement in human plasma
Jones, D. P., Carlson, J. L., Samiec, P. S., Sternberg, P., Mody, V. C., Reed, R. L., & Brown, L. A. S. (1998) · Clinica Chimica Acta · doi:10.1016/s0009-8981(98)00089-8 · Cited by 484
Metabolic Responses to Reductive Stress
Xiao, W., & Loscalzo, J. (2020) · Antioxidants & Redox Signaling · doi:10.1089/ars.2019.7803 · Cited by 462
Adams, J. B., Audhya, T., McDonough-Means, S., Rubin, R. A., Quig, D., Geis, E., Gehn, E., Loresto, M., Mitchell, J., Atwood, S., et al. (2011) · Nutrition & Metabolism · doi:10.1186/1743-7075-8-34 · Cited by 442
What do we know about the reduction of Pt(IV) pro-drugs?
Wexselblatt, E., & Gibson, D. (2012) · Journal of Inorganic Biochemistry · doi:10.1016/j.jinorgbio.2012.06.013 · Cited by 370
Metadata
- Authors
- Michelet, F., Gueguen, R., Leroy, P., Wellman, M., Nicolas, A., & Siest, G.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-10-01
- Volume
- 41
- Issue
- 10
- Pages
- 1509-1517
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2113699453
- Cited by
- 326
- References
- 28
- FWCI
- 2.68
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Michelet, F., Gueguen, R., Leroy, P., Wellman, M., Nicolas, A., & Siest, G. (1995). Blood and plasma glutathione measured in healthy subjects by HPLC: relation to sex, aging, biological variables, and life habits. Clinical Chemistry. https://doi.org/10.1093/clinchem/41.10.1509
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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