Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients
Open accessAbstract
Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, and it is involved in numerous malignancies. Reduced glutathione (GSH) is considered to be one of the most important scavengers of reactive oxygen species (ROS), and its ratio with oxidised glutathione (GSSG) may be used as a marker of oxidative stress. The main aim of this study was to determine GSH:GSSG ratio in the blood serum of paediatric cancer patients to use this ratio as a potential marker of oxidative stress. The whole procedure was optimised and the recoveries for both substances were greater than 80% under the optimised conditions. We analysed a group of paediatric patients (n=116) with various types of cancer, including neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract tumour, lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma and retinoblastoma. We simultaneously determined the levels of reduced and oxidised glutathione, and thus, its ratio in the blood serum of the patients. The highest ratio was observed in retinoblastoma patients and the lowest in anaplastic ependymoma. We were able to distinguish between the diagnoses based on the results of the obtained GSH:GSSG ratio.
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In healthy human RBCs, 90%-95% of glutathione is present in the reduced form GSH.
“In healthy human RBCs, 90%–95% of glutathione is present in the reduced form GSH (200) that can be utilized for the reduction of ascorbate, oxidized proteins, and oxidized lipids (184).”
From Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia (2017) · cites this paper as [200]
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1 retracted
Cysteine and Glutathione Secretion in Response to Protein Disulfide Bond Formation in the ER
Carelli, S., Ceriotti, A., Cabibbo, A., Fassina, G., Ruvo, M., & Sitia, R. (1997) · Science · doi:10.1126/science.277.5332.1681 · Cited by 127 · see citing works
RetractedFree Radicals in the Physiological Control of Cell Function
Dröge, W. (2002) · Physiological Reviews · doi:10.1152/physrev.00018.2001 · Cited by 9,855 · see citing works
Free radicals, metals and antioxidants in oxidative stress-induced cancer
Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M., & Mazur, M. (2006) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2005.12.009 · Cited by 6,680 · see citing works
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control
Noctor, G., & Foyer, C. H. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.249 · Cited by 5,722 · see citing works
Oxyl radicals, redox-sensitive signalling cascades and antioxidants
Genestra, M. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2007.04.009 · Cited by 4,930 · see citing works
Hayes, J. D., & Pulford, D. J. (1995) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239509083491 · Cited by 3,471 · see citing works
The importance of glutathione in human disease
Townsend, D. M., Tew, K. D., & Tapiero, H. (2003) · Biomedicine & Pharmacotherapy · doi:10.1016/s0753-3322(03)00043-x · Cited by 2,009 · see citing works
Biomarkers of Oxidative Damage in Human Disease
Dalle-Donne, I., Rossi, R., Colombo, R., Giustarini, D., & Milzani, A. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2005.061408 · Cited by 1,703 · see citing works
Griffith, O. W., & Meister, A. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35980-5 · Cited by 1,683 · see citing works
Hayes, J. D., & McLellan, L. I. (1999) · Free Radical Research · doi:10.1080/10715769900300851 · Cited by 1,528 · see citing works
Assessment of stress during handling and transport.
Grandin, T. (1997) · Journal of Animal Science · doi:10.2527/1997.751249x · Cited by 1,151 · see citing works
Glutathione and Related γ-Glutamyl Compounds: Biosynthesis and Utilization
Meister, A., & Tate, S. S. (1976) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.45.070176.003015 · Cited by 839 · see citing works
Samiec, P. S., Drews‐Botsch, C., Flagg, E. W., Kurtz, J. C., Sternberg, P., Reed, R. L., & Jones, D. P. (1998) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00286-4 · Cited by 493 · see citing works
Validation of chromatographic methods in biomedical analysis viewpoint and discussion
Causon, R. C. (1997) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(96)00297-6 · Cited by 474 · see citing works
Cerebrospinal fluid cytology in patients with cancer
Glantz, M., Cole, B. F., Glantz, L. K., Cobb, J. L., Mills, P., Lekos, A., Walters, B. C., & Recht, L. D. (1998) · Cancer · doi:10.1002/(sici)1097-0142(19980215)82:4 · Cited by 457 · see citing works
The effects of stress and aging on glutathione metabolism
Maher, P. A. (2005) · Ageing Research Reviews · doi:10.1016/j.arr.2005.02.005 · Cited by 426 · see citing works
Dopamine turnover and glutathione oxidation: implications for Parkinson disease.
Spina, M. B., & Cohen, G. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.4.1398 · Cited by 397 · see citing works
Brain oxidative stress in a triple-transgenic mouse model of Alzheimer disease
Resende, R., Moreira, P. I., Proença, T., Deshpande, A., Busciglio, J., Pereira, C., & Oliveira, C. R. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.03.012 · Cited by 343 · see citing works
Michelet, F., Gueguen, R., Leroy, P., et al. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.10.1509 · Cited by 326 · see citing works
Glutathione Metabolism during Aging and in Alzheimer Disease
LIU, H., Wang, H., Shenvi, S. V., Hagen, T. M., & LIU, R. (2004) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1297.059 · Cited by 258 · see citing works
Yoshida, K., Hirokawa, J., Tagami, S., Kawakami, Y., Urata, Y., & Kondo, T. (1995) · Diabetologia · doi:10.1007/bf00400095 · Cited by 248 · see citing works
Pastore, A., Massoud, R., Motti, C., et al. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.4.825 · Cited by 241 · see citing works
Blood glutathione concentrations in a large-scale human study
Richie, J. P., Skowronski, L., Abraham, P., & Leutzinger, Y. (1996) · Clinical Chemistry · doi:10.1093/clinchem/42.1.64 · Cited by 233 · see citing works
Chai, Y. C., Ashraf, S. M., Rokutan, K., Johnston, R. B., & Thomas, J. A. (1994) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1994.1167 · Cited by 228 · see citing works
Pro-oxidant shift in glutathione redox state during aging
Rebrin, I., & Sohal, R. S. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.06.001 · Cited by 194 · see citing works
Intracellular cysteine and glutathione delivery systems.
Meister, A., Anderson, M. E., & Hwang, O. (1986) · Journal of the American College of Nutrition · doi:10.1080/07315724.1986.10720121 · Cited by 192 · see citing works
The glutathione defense system in the pathogenesis of rheumatoid arthritis
Hassan, M. N. M., Hadi, R. A., Al-Rawi, Z. S., Padrón, V. A., & STOHS, S. J. (2001) · Journal of Applied Toxicology · doi:10.1002/jat.736 · Cited by 187 · see citing works
Potěšil, D., Petrlová, J., Adam, V., Vacek, J., Klejdus, B., Zehnálek, J., Trnková, L., Havel, L., & Kizek, R. (2005) · Journal of Chromatography A · doi:10.1016/j.chroma.2005.06.019 · Cited by 182 · see citing works
Self-citationLipid, protein, DNA oxidation and antioxidant status in rheumatoid arthritis
Seven, A., Güzel, S., Aslan, M., & Hamuryudan, V. (2008) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2008.01.029 · Cited by 168 · see citing works
Proteasome Inhibition Induces Glutathione Synthesis and Protects Cells from Oxidative Stress
Yamamoto, N., Sawada, H., Izumi, Y., Kume, T., Katsuki, H., Shimohama, S., & Akaike, A. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m603712200 · Cited by 133 · see citing works
A study of glutathione status in the blood and tissues of patients with breast cancer
Yeh, C., Hou, M., Wu, S., et al. (2005) · Cell Biochemistry and Function · doi:10.1002/cbf.1275 · Cited by 117 · see citing works
The progression of Parkinson disease
Lang, A. E. (2007) · Neurology · doi:10.1212/01.wnl.0000257110.91041.5d · Cited by 116 · see citing works
Dinçer, Y., Akçay, T., Alademir, Z., & ILKOVA, H. M. (2002) · Metabolism · doi:10.1053/meta.2002.35192 · Cited by 116 · see citing works
Chromatographic and mass spectrometric analysis of glutathione in biological samples
Iwasaki, Y., Saito, Y., Nakano, Y., Mochizuki, K., Sakata, O., Ito, R., Saito, K., & Nakazawa, H. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.07.001 · Cited by 111 · see citing works
Lochman, P., Adam, T., Friedecký, D., Hlídková, E., & Škopková, Z. (2003) · Electrophoresis · doi:10.1002/elps.200390154 · Cited by 107 · see citing works
Petrlová, J., Mikelová, R., Stejskal, K., Kleckerová, A., Zítka, O., Petřek, J., Havel, L., Zehnálek, J., Adam, V., Trnková, L., et al. (2006) · Journal of Separation Science · doi:10.1002/jssc.200500425 · Cited by 87 · see citing works
Self-citationReduced glutathione system: Role in cancer development, prevention and treatment (Review)
Locigno, R., & Castronovo, V. (2001) · International Journal of Oncology · doi:10.3892/ijo.19.2.221 · Cited by 85 · see citing works
Glutathione concentration in oral cancer tissues
Wong, D. Y.-K., Hsiao, Y., Poon, C., et al. (1994) · Cancer Letters · doi:10.1016/0304-3835(94)90191-0 · Cited by 78 · see citing works
Margutti, P., Matarrese, P., Conti, F., Colasanti, T., Delunardo, F., Capozzi, A., Garofalo, T., Profumo, E., Riganò, R., Siracusano, A., et al. (2007) · Blood · doi:10.1182/blood-2007-05-092825 · Cited by 73 · see citing works
Rebrin, I., Bayne, A.-C. V., Mockett, R. J., Orr, W. C., & Sohal, R. S. (2004) · Biochemical Journal · doi:10.1042/bj20040506 · Cited by 70 · see citing works
Analysis of the thiol status of peripheral blood leukocytes in rheumatoid arthritis patients
Pedersen-Lane, J., Zurier, R. B., & Lawrence, D. A. (2007) · Journal of Leukocyte Biology · doi:10.1189/jlb.0806533 · Cited by 67 · see citing works
The glutathione precursor N-acetylcysteine improves immune function in postmenopausal women
Arranz, L., Fernández, C., Rodríguez, A., Ribera, J. M., & la Fuente, M. D. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.07.014 · Cited by 62 · see citing works
Klejdus, B., Zehnálek, J., Adam, V., Petřek, J., Kizek, R., Vacek, J., Trnková, L., Rozik, R., Havel, L., & Kubáň, V. (2004) · Analytica Chimica Acta · doi:10.1016/j.aca.2004.02.060 · Cited by 53 · see citing works
Self-citationA., C., Motz, E., Cavarape, A., Lizzio, S., Russo, A., Quatraro, A., & Giugliano, D. (1997) · Journal of Diabetes and its Complications · doi:10.1016/s1056-8727(97)00021-4 · Cited by 44 · see citing works
Relationship between colorectal cancer glutathione levels and patient survival
BARRANCO, S. C., Perry, R. R., Durm, M., et al. (2000) · Diseases of the Colon & Rectum · doi:10.1007/bf02236562 · Cited by 44 · see citing works
Karelson, E., Mahlapuu, R., Zilmer, M., et al. (2002) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2002.tb04696.x · Cited by 32 · see citing works
Dietary Polyunsaturated Fatty Acids and Aging Modulate Glutathione-Related Antioxidants in Rat Liver
Christon, R., Haloui, R. B., & Durand, G. (1995) · Journal of Nutrition · doi:10.1093/jn/125.12.3062 · Cited by 32 · see citing works
Effect of glutathione (GSH) depletion on DNA damage and blood chemistry in aged and young rats
Hashimoto, K., Takasaki, W., Yamoto, T., Manabe, S., Sato, I., & Tsuda, S. (2008) · The Journal of Toxicological Sciences · doi:10.2131/jts.33.421 · Cited by 30 · see citing works
Decreased plasma glutathione in cancer of the uterine cervix
Kumar, A., Sharma, S. K., Pundir, C. S., et al. (1995) · Cancer Letters · doi:10.1016/0304-3835(95)03832-h · Cited by 29 · see citing works
Strategies for engineered negligible senescence : why genuine control of aging may be foreseeable
de Grey, A. D. N. J. (2004) · New York Academy of Sciences eBooks · Cited by 26 · see citing works
Zítka, O., Húska, D., Křížková, S., Adam, V., Chavis, G. J., Trnková, L., Horna, A., Hubálek, J., & Kizek, R. (2007) · Sensors · doi:10.3390/s7071256 · Cited by 25 · see citing works
Self-citationOxidative stress and endothelial damage in patients with asymptomatic carotid atherosclerosis
Signorelli, S. S., Neri, S., Pino, L. D., et al. (2001) · Clinical and Experimental Medicine · doi:10.1007/s10238-001-8002-7 · Cited by 20 · see citing works
High-performance liquid chromatographic measurement of exogenous thiosulfate in urine and plasma
SHEA, M. A., & Howell, S. B. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90211-2 · Cited by 18 · see citing works
Cell signaling, transcription and translation as therapeutic targets
Diederich, M. F. (2002) · Biochemical Pharmacology · doi:10.1016/s0006-2952(02)01178-4 · Cited by 10 · see citing works
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Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia
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- Authors
- ZITKA, O., SKALICKOVA, S., GUMULEC, J., MASARIK, M., ADAM, V., HUBALEK, J., TRNKOVA, L., KRUSEOVA, J., ECKSCHLAGER, T., & KIZEK, R.
- Venue
- Oncology Letters
- Publisher
- Spandidos Publications
- Published
- 2012
- Volume
- 4
- Issue
- 6
- Pages
- 1247-1253
- ISSN
- 1792-1074 (ISSN-L)
- 1792-1082
- PMC
- PMC3506742
- OpenAlex
- W2002805664
- Cited by
- 664
- References
- 54
- Citations by year
- 2026: 48 · 2025: 53 · 2024: 66 · 2023: 77 · 2022: 55 · 2021: 60 · 2020: 57 · 2019: 78 · 2018: 51 · 2017: 48 · 2016: 30 · 2015: 24 · 2014: 11 · 2013: 6
- FWCI
- 9.17
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Oncology Letters (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Central European Institute of Technology (CZ.1.05/1.1.00/02.0068., CZ.1.05/1.1.00/02)
- Topics
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Keywords
- GSH/GSSG ratio, oxidative stress, reduced glutathione, glutathione disulfide, childhood cancer, serum biomarkers, retinoblastoma, anaplastic ependymoma, neuroblastoma, rhabdomyosarcoma, Wilms tumor, Ewing sarcoma, medulloblastoma, germ cell tumors, diagnostic biomarkers
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