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Automated assay of oxygen radical absorbance capacity with the COBAS FARA II
Abstract
Reactive oxygen species are of interest in biology and medicine because of evidence relating them to aging and disease processes. A relatively simple but sensitive and reliable method for quantifying the oxygen radical absorbance capacity (ORAC) of antioxidants in biological tissues has been automated for use with the COBAS FARA II centrifugal analyzer with a fluorescence-measuring attachment. In this assay, beta-phycoerythrin (beta-PE) is used as an indicator protein, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) as a peroxyl radical generator, and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) as a calibrator for antioxidant activity. This assay is unique because the reaction goes to completion so that both inhibition time and inhibition degree are considered in quantifying ORAC (micromoles of Trolox equivalent per liter or per gram of tissue). This method can be used not only for serum but also other tissue and food samples and is suitable for application to a range of nutritional and clinical conditions.
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Cited by
Antioxidant activity applying an improved ABTS radical cation decolorization assay
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00315-3 · Cited by 26,165
Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., & Jacob, R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0262256 · Cited by 1,455
Self-citationExtending Applicability of the Oxygen Radical Absorbance Capacity (ORAC−Fluorescein) Assay
Dávalos, A., Gómez-Cordovés, C., & Bartolomé, B. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0305231 · Cited by 1,207
Joseph, J. A., Shukitt-Hale, B., Denisova, N. A., Bielinski, D., Martin, A., McEwen, J. J., & Bickford, P. C. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-18-08114.1999 · Cited by 980
Assessment of antioxidant capacity in vitro and in vivo
Niki, E. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.04.016 · Cited by 873
Metadata
- Authors
- Cao, G., Verdon, C. P., Wu, A. H., Wang, H., & Prior, R. L.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1995-12-01
- Volume
- 41
- Issue
- 12
- Pages
- 1738-1744
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2106071093
- Cited by
- 386
- References
- 0
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cao, G., Verdon, C. P., Wu, A. H., Wang, H., & Prior, R. L. (1995). Automated assay of oxygen radical absorbance capacity with the COBAS FARA II. Clinical Chemistry. https://doi.org/10.1093/clinchem/41.12.1738
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