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Comparison of different analytical methods for assessing total antioxidant capacity of human serum
Abstract
Three assays were compared for the determination of total antioxidant capacity in human serum: the oxygen radical absorbance capacity (ORAC) assay, the Randox Trolox-equivalent antioxidant capacity (Randox-TEAC) assay, and the ferric reducing ability (FRAP) assay. There was a weak but significant linear correlation between serum ORAC and serum FRAP. There was no correlation either between serum ORAC and serum TEAC or between serum FRAP and serum TEAC. The effect of dilution on the serum TEAC value and the use of inhibition percentage at a fixed time, without considering the length of inhibition time in the quantitation of results, adversely affected the Randox-TEAC assay. The FRAP assay is simple and inexpensive but does not measure the SH-group-containing antioxidants. The ORAC assay has high specificity and responds to numerous antioxidants. By utilizing different extraction techniques in the ORAC assay, one can remove serum proteins and also make some gross differentiation between aqueous and lipid-soluble antioxidants. However, the ORAC assay requires ∼60 min more than the FRAP or Randox-TEAC assay to quantitate results.
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Cited by
The Chemistry behind Antioxidant Capacity Assays
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Kohen, R., & Nyska, A. (2002) · Toxicologic Pathology · doi:10.1080/01926230290166724 · Cited by 2,178
Antioxidant activity and phenolic compounds in 32 selected herbs
WOJDYLO, A., OSZMIANSKI, J., & CZEMERYS, R. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2007.04.038 · Cited by 2,023
Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., & Jacob, R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0262256 · Cited by 1,455
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Metadata
- Authors
- Cao, G., & Prior, R. L.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-06-01
- Volume
- 44
- Issue
- 6
- Pages
- 1309-1315
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2111577111
- Cited by
- 902
- References
- 0
- FWCI
- 12.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cao, G., & Prior, R. L. (1998). Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clinical Chemistry. https://doi.org/10.1093/clinchem/44.6.1309
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