The effects of feeding and withholding food on the canine small intestinal microbiota
Open accessAbstract
Prolonged lack of enteral feeding has a negative impact on gut physiology, potentially via microbiota modulation. The aims were to investigate the impact of fasting and post-prandial changes in canine jejunal microbiota. To study post-prandial effects, jejunal brushings were analyzed in 8 healthy fistulated dogs 15 min before feeding (baseline) and hourly for 8 h after feeding. To study effects of withholding food (WF), daily samples were collected for 15 days from 5 dogs. The first 5 days (PRE) dogs were fed regular diet. Food was withheld the next 5 days (days 6-10). For days 11-15 (POST), the original diet was reintroduced. Microbiota was characterized via denaturing gradient gel electrophoresis and 454-pyrosequencing of 16S rRNA genes. In the post-prandial study, no changes in microbiome structure were seen after feeding (ANOSIM, P = 0.28), but Betaproteobacteria (P = 0.04) and Bacteroidales decreased compared to baseline. Species richness decreased by 300 min (P = 0.04). During WF, microbiota structure differed from PRE and POST period (P = 0.001). During WF, species richness did not vary over time (P = 0.69). In conclusion, a prolonged period of food withholding results in altered jejunal microbiota. How these changes affect the microbiota metabolism warrants further studies.
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Time-restricted feeding in mice and repeated prolonged cycles of feeding and withholding food in dogs cause a change in the overall gut microbiome that appears driven by changes in the abundance of species that differ by their preferred fermentable substrates.
“Time-restricted feeding in mice [79] and repeated prolonged cycles of feeding and withholding food in dogs [80] have all been shown to cause a change in the overall gut microbiome that appears to be driven by changes in the abundance of species that differ by their preferred fermentable substrates (e.g., host-vs. diet-derived glycans).”
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“Time-restricted feeding in mice [79] and repeated prolonged cycles of feeding and withholding food in dogs [80] have all been shown to cause a change in the overall gut microbiome that appears to be driven by changes in the abundance of species that differ by their preferred fermentable substrates (e.”
Background
- Authors
- Kasiraj, A. C., Harmoinen, J., Isaiah, A., Westermarck, E., Steiner, J. M., Spillmann, T., & Suchodolski, J. S.
- Affiliations
- Texas A&M University (US)
- University of Helsinki (FI)
- Corresponding author
- Suchodolski, Jan S.
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-04-21
- Volume
- 92
- Issue
- 6
- Pages
- fiw085
- ISSN
- 0168-6496 (ISSN-L)
- 1574-6941
- OpenAlex
- W2336313813
- Cited by
- 36
- References
- 33
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2021: 17 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 1
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- canine small intestine, jejunal microbiota, fasting, enteral nutrition, Betaproteobacteria, Bacteroidales, species richness, gut microbiota, dogs
- MeSH terms
- Gastrointestinal Microbiome, Animals, Bacteroides, Diet, Dogs, Fasting, Food, Intestine, Small, Jejunum, RNA, Ribosomal, 16S, Betaproteobacteria, Denaturing Gradient Gel Electrophoresis
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