Schizosaccharomyces pombe Ppr10 is required for mitochondrial translation

Liu, Zecheng; Li, Yan; Xie, Wanqiu; Huang, Ying

doi:10.1093/femsle/fnaa170

Cited by 10Mitochondrial Function and PathologyFungal and yeast genetics researchRNA Research and Splicing

Schizosaccharomyces pombe Ppr10 is required for mitochondrial translation

Liu, Zecheng, Li, Yan, Xie, Wanqiu, Huang, Ying·2020·FEMS Microbiology Letters

DOI 10.1093/femsle/fnaa170

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Abstract

The mitochondrial genome encodes key components of the oxidative phosphorylation (OXPHOS) system, whose expression is essential for mitochondrial functions. We have previously shown that deletion of the Schizosaccharomyces pombe ppr10 encoding a pentatricopeptide repeat protein severely reduces the mature levels of intron-containing mitochondrial transcripts cox1 and cob1, and severely impairs mitochondrial translation. In this study, we examined the possibility that the reduced levels of Cox1 and Cob1 proteins in cells were due to lowered levels of cox1 and cob1 mRNAs. We found that deletion of ppr10 did not affect the levels of mature cox1 and cob1 mRNAs in a mitochondrial intronless background. However, synthesis of Cox1 and Cob1 proteins were still severely affected by deletion of ppr10 in a mitochondrial intronless background. Consistent with this, we found that deletion of mitochondrial introns could not rescue the respiratory growth defect of Δppr10 cells. Our results reveal that Ppr10 is not required for the stability of cox1 and cob1 mRNAs, and provide further support for the idea that Ppr10 plays a critical role in mitochondrial translation.

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SpPpr10p mediates the association of translation initiation factors SpMti2p, SpMti3p, and SpMpa1 with the small mitoribosome subunit.
“SpPpr10p was reported to mediate the association of translation initiation factors (SpMti2p, SpMti3p, and the SpMpa1 matrix protein) with the small mitoribosome subunit (mt-SSU) (Liu et al. 2020; Luo et al. 2021).”
From Candida albicans PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Liu et al. 2020

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Authors: Liu, Z., Li, Y., Xie, W., & Huang, Y.
Affiliations: Nanjing Normal University (CN)
Corresponding author: Huang, Ying
ORCID: Li, Yan
Huang, Ying
Venue: FEMS Microbiology Letters
Publisher: Oxford University Press (OUP)
Published: 2020-10
Volume: 367
Issue: 19
ISSN: 0378-1097 (ISSN-L)
1574-6968
DOI: 10.1093/femsle/fnaa170
OpenAlex: W3092132433
Cited by: 10
References: 35
Citations by year: 2026: 2 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 1
FWCI: 0.41
Type: journal article
Language: en
Open access: closed
Licence: academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations: FEMS Microbiology Letters (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation of China (31770810)
Priority Academic Program Development of Jiangsu Higher Education Institutions
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: pentatricopeptide repeat proteins, mitochondrial translation, Schizosaccharomyces pombe, mitochondrial COI, mitochondrial introns, mRNA stability, oxidative phosphorylation, mitochondrial genome
MeSH terms: Mitochondria, Schizosaccharomyces, Protein Biosynthesis, RNA-Binding Proteins, Schizosaccharomyces pombe Proteins

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Schizosaccharomyces pombe Ppr10 is required for mitochondrial translation

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