The Schizosaccharomyces pombe PPR protein Ppr10 associates with a novel protein Mpa1 and acts as a mitochondrial translational activator
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The pentatricopeptide repeat (PPR) proteins characterized by tandem repeats of a degenerate 35-amino-acid motif function in all aspects of organellar RNA metabolism, many of which are essential for organellar gene expression. In this study, we report the characterization of a fission yeast Schizosaccharomyces pombe PPR protein, Ppr10 and a novel Ppr10-associated protein, designated Mpa1. The ppr10 deletion mutant exhibits growth defects in respiratory media, and is dramatically impaired for viability during the late-stationary phase. Deletion of ppr10 affects the accumulation of specific mitochondrial mRNAs. Furthermore, deletion of ppr10 severely impairs mitochondrial protein synthesis, suggesting that Ppr10 plays a general role in mitochondrial protein synthesis. Ppr10 interacts with Mpa1 in vivo and in vitro and the two proteins colocalize in the mitochondrial matrix. The ppr10 and mpa1 deletion mutants exhibit very similar phenotypes. One of Mpa1's functions is to maintain the normal protein level of Ppr10 protein by protecting it from degradation by the mitochondrial matrix protease Lon1. Our findings suggest that Ppr10 functions as a general mitochondrial translational activator, likely through interaction with mitochondrial mRNAs and mitochondrial translation initiation factor Mti2, and that Ppr10 requires Mpa1 association for stability and function.
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CaPpr7 shows functional similarity to ScMrx1p, SpPpr10p, and Ncu08674 of N. crassa.
“Although CaPpr7 is not clearly orthologous to any known fungal PPR proteins, it shows some functional similarity to ScMrx1p (Kehrein et al. 2015), SpPpr10p (Wang et al. 2017), and Ncu08674 of N. crassa (Solotoff et al. 2015).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Wang et al. 2017
Deletion of SpPPR10 causes a reduction in some mitochondrial transcripts and a partial loss of mtDNA.
“Deletion of the SpPPR10 gene causes a reduction in some mitochondrial transcripts and a partial loss of mtDNA (Wang et al. 2017).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Wang et al. 2017
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Identification and analysis of iron transporters from the fission yeast Schizosaccharomyces pombe
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Schizosaccharomyces pombe Sls1 is primarily required for cox1 mRNA translation
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E3 ubiquitin ligase Hul6 modulates iron-dependent metabolism by regulating Php4 stability
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Lon1 protease controls pentatricopeptide-repeat-mediated RNA processing in Arabidopsis mitochondria
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Genetic and environmental determinants of multicellular-like phenotypes in fission yeast
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- Authors
- Wang, Y., Yan, J., Zhang, Q., Ma, X., Zhang, J., Su, M., Wang, X., & Huang, Y.
- Affiliations
- Nanjing Normal University (CN)
- Corresponding author
- Huang, Ying
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-02-22
- Volume
- 45
- Issue
- 6
- Pages
- 3323-3340
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- PMC
- PMC5389468
- OpenAlex
- W2589975971
- Cited by
- 34
- References
- 84
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 6 · 2023: 1 · 2022: 8 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 7
- FWCI
- 1.12
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31470778)
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- pentatricopeptide repeat proteins, mitochondrial translation, mitochondrial mRNA, protein stability, Schizosaccharomyces pombe, protein-protein interaction
- MeSH terms
- RNA, Mitochondrial, Carrier Proteins, DNA, Mitochondrial, Mitochondria, Mutation, Phenotype, RNA, RNA, Messenger, Schizosaccharomyces, Protein Biosynthesis, RNA-Binding Proteins, Mitochondrial Proteins, Schizosaccharomyces pombe Proteins, Eukaryotic Initiation Factors
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